We narrowed to 7 results for: antibody

This protocol describes how to run an indirect ELISA against a purified antigen to demonstrate the expected antibody dose response.

This protocol describes the basic steps for fixing and staining cells in culture with a primary antibody against a target protein and a fluorescent secondary antibody.

This protocol describes the basic steps for lysing cells, determining total protein concentration in the lysate, running a precast SDS-PAGE gel, and immunoblotting.

Lab protocol for transfection of HEK293 cells to produce recombinant antibody.

Lab protocol to determine purity and concentration of recombinant antibodies using ready-to-use biosafe Coomassie stain and ImageJ software.

This protocol describes how to affinity purify recombinant antibodies from cell culture supernatant using Protein A or Protein G columns.

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.