We narrowed to 242 results for: kit
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TypeCollection... Gateway-compatible Kit for Neurodegenerative Disorders - Nicholas Cole Lab. A kit consisting of plasmids...and Cre expression plasmids. Large collections or kits are described below . ID Plasmid Description Gene...compartment-specific biotin labeling in zebrafish. pGCS Vector Kit - Hai-Ning Du Lab. A set of pCS2+ backbone-based ...Construct Set - Amro Hamdoun Lab. A useful “starter kit” for screening fluorescent protein fusion expression...repository at the Karlsruhe Institute of Technology (KIT) for the distribution of zebrafish lines from European...model potential cancer drivers in vivo. Biotagging toolkit - Tatjana Sauka-Spengler Lab. A genetic binary ...
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What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry
TypeBlog Post...guanidine thiocyanate (used in many plasmid purification kits), which can dramatically reduce the A260/230 ratio...Plasmid DNA? Selecting Your Plasmid Purification Kit Resources on Addgene.org DNA Quantification Protocol... -
Plasmids 101: Gateway Cloning
TypeBlog Post...Lentiviral Expression Systems (MuLE) Kit, the MultiSite Gateway cloning kit, and MultiSite Gateway plasmids... -
Viral Production at Addgene
TypeBlog Post...mycoplasma contamination using mycoplasma detection kits. Cell line is maintained for ~20 passages before...prepared using endotoxin-free plasmid purification kits. Figure 4: Schematic of the barcode ... -
Troubleshooting and Optimizing a Western Blot
TypeBlog Post...working, consider using a subcellular fractionation kit or a lysing protocol developed for isolating proteins...based lysing protocol Subcellular fractional kit or lysing protocol Not enough target Run protein...Chemilumenescence: use a more sensitive exposure kit Too much signal Antibody dilutions too high...Chemilumenescence: use a less sensitive exposure kit No signal variation in highly expressed proteins... -
Cancer Research Plasmids and Resources
TypeCollection...transcription factor and tumor suppressor. Wnt Coming soon! Kits and Libraries for Cancer Research Broad Target Accelerator...of a “pan-cancer” analyses. Cancer Pathways ORFs Kit : Set of vectors from the Sabatini & Wood Labs containing...Tackling Cancers’ Drug Resistance with a New Screening Kit (David Sabatini & Kris Wood) National Cancer Institute... -
TALEN Engineering
TypeCollection... Genome Engineering TALEN Kits TALengineering Reagents Joung Lab TAL Effector Engineering...are provided below: Joung Lab REAL Assembly TALEN Kit Individual REAL TALE Repeat Plasmids TALEN Expression...Plasmids compatible with the REAL Assembly TALEN Kit: TALE-LSD1 plasmids for epigenome editing... -
mTOR Pathway
TypeCollection...Pathway ORF Kit Ras Pathway p53 Pathway Tackling Cancers’ Drug Resistance with a New Screening Kit Background...Pathway ORF Kit (Sabatini & Wood) Tackling Cancers’ Drug Resistance with a New Screening Kit (from David... -
Synthetic Biology, Artificial Chromosomes, iGEM & More - Interview with Tom Ellis
TypeBlog Post...Plasmids from the Ellis Lab Find Synthetic Biology Kits Browse Our Synthetic Biology Pages ... -
Antibodies 101: Beyond Surface Labeling
TypeBlog Post...targeted markers. Some commercially available fix/perm kits allow for labeling of cytosolic proteins, as well...located inside the nucleus, a different fix/perm kit is required (to be precise, the fixing solution is... -
Cre-Lox and Other Site-Specific Recombinases
TypeCollection...expressing Cre-dependent Cas9. Kits Kit Name Type PI Description dRMCE Plasmid Kit Genome Engineering Rolf Zeller...Empty Backbones Reporter Plasmids Pooled Libraries/Kits Site-specific recombinase (SSR) systems, like Cre-lox...recombinase-mediated cassette exchange (dRMCE) plasmid kit to re-engineer conditional alleles in mouse embryonic... -
Editor's Choice, September 2016
TypeBlog Post...Good Cover Letter Multiple Plasmids at a Low Price: Kits from Addgene Savvy Advocates Needed to Navigate ... -
Plant Plasmids and Resources
TypeCollection...Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized SpCas9...plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing, and silencing...Lohmann. MoClo Plant Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs...individual plasmids, Addgene carries several useful toolkits and plasmid collections for use in plants. The...plant CRISPR resources include: A Multipurpose Toolkit enables targeted, specific modification of monocot...wheat, and barley - Daniel Voytas. A CRISPR/Cas9 Toolkit for use in a variety of plant species (e.g. maize...Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes CRISPR/Cas nucleases, base ... -
CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9
TypeBlog Post...in this blog post. The pDGE Dicot Genome Editing Kit from the Stuttmann lab contains a variety of Agrobacterium-compatible...from the Yiping Qi lab and the MoClo Plant Parts Kit from the Patron Lab. As with all plasmids in the...described above, Addgene carries several useful CRISPR toolkits for creating plant expression plasmids, including... -
What's New in CRISPR - September 2019
TypeBlog Post... the paper in bioRxiv Find the GeneWeld vector kit CasX, a small Cas protein, is active in E. coli ... -
Fluorescent Proteins 101: Fluorescent Biosensors
TypeBlog Post... FRET biosensors can be created using the cpFRET kit created by Olivier Pertz’s lab. Other biosensors ... -
Plasmids 101: Positive and Negative Selection for Plasmid Cloning
TypeBlog Post... Markus Ralser’s lab created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. ... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog Post...biologists bought the pricey stuff in 50 ul aliquots in a kit with a huge amount of wasteful packaging. Turns ... -
Botman-Teusink Yeast FP Collection
TypeCollection...Additional Resources Addgene Kits MoClo-YTK Yeast Gateway Kit Multiplex Yeast Toolkit (MYT) Markerless Yeast...Localization and Overexpression (MyLO) CRISPR-Cas9 Toolkit Other Addgene Plasmids Bas Teusink Lab Materials... -
Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps
TypeProtocol...Extraction Without a Kit Extract RNA from tissues or cells without a commercial kit Plasmid Cloning Protocols...