We narrowed to 537 results for: try
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TypeBlog Post...great place to start if you’re new to the topic. Or, try the search bar Now, you can search for the articles...
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New Podcast Segment: Hot Plasmids
TypeBlog Post...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment... -
Kit Free RNA Extraction
TypeProtocol...instead of incubating at -20°C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 ... -
Newly Updated AAV Data Hub!
TypeBlog Post...your data, too! Using the Data Hub If you're trying to decide which AAV is right for your experiment... -
Protocol - How to Purify DNA from an Agarose Gel
TypeProtocol...protect the UV box from being cut by the razor blade. Try to get as little excess gel around the band as possible... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog Post... The PAM sequence is of particular concern when trying to edit a gene using homology directed repair, ...of reagents available through Addgene! If you're trying to decide which CRISPR tools are best for your ... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...to probe interactions in locations of interest. Try BioID for your proximity labeling experiment! APEX...favorite protein or RNA motif, give these methods a try. References: Branon, Tess C., et al. "Efficient...antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have...classic antibody based affinity purification/mass spectrometry methods. BioID: a promiscuous biotin ligase...purified using streptavidin and separated via flow cytometry. Find plasmids for EXCELL here! Since BioID was... -
Filming Science Videos in the Age of Social Distancing
TypeBlog Post...taking a look at what works and what doesn’t, and trying all over again. Here’s that process! Figure...Shot without anyone else in the office. I try to build every setup with an eye to the future. I... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...the world so it feeds a lot people. This lab is trying to improve homologous recombination efficiency ...injection into adult female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive... -
Getting the Most from Your Lentiviral Transduction
TypeBlog Post...lentiviral preps unless absolutely necessary. Many labs try to use freshly collected lentivirus for downstream...well to other cell types. To overcome this issue, try transducing with a range of dilutions of your lentiviral...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels... -
Thoughts on the Future of Research 2015 Boston Symposium
TypeBlog Post... associations across different universities are trying to compile more comprehensive demographic information...beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career... -
Bringing Sustainable Practices to the Lab: Easy Action Items
TypeBlog Post...addition to doing some of the quick things above, try joining or starting a sustainability project. This... -
How Do I Place an Addgene Order Using a PO Number?
TypeBlog Post...online is a little trickier -- but at Addgene, we try to make the process as smooth and simple as possible... -
Science Communication for Everyone
TypeBlog Post... should also be able to understand what you are trying to convey without having to extensively use Google... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog Post...Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas... -
Designer PUF Proteins for Any RNA Target
TypeBlog Post...Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves, she does... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog Post... does have one special tip for anyone looking to try this at home in their own lab: be very careful with... -
Transferable Skills Guide: Conflict Resolution
TypeBlog Post... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties... -
A Control for All Seasons
TypeBlog Post... knockout. If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on. Flow cytometry In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this blog post will ... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog Post...GATC) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes (ex: ClaI...CCWGG) Preparing unmethylated DNA, important when trying to cut with certain restriction enzymes that are...