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Welcome to the Addgene blog! Here you'll find all the info you need on plasmids, CRISPR, viral vectors, fluorescent proteins, science careers, and more.

In our new Hot Plasmids segment on the Addgene podcast, you can quickly learn about a variety of new plasmid technologies from our quarterly newsletter.

We are excited to officially re-launch our redesigned AAV Data Hub, with easy submission and DOIs to help share science!

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

There are many method to identify protein-protein interactions. Learn about different method of proximity labeling based on biotin-streptavidin interactions.

Learn more about unique organisms that are being developed as model systems in this blog post.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

Read our highlights from the Future of Research Symposium Boston 2015 and how scientists are tackling structural problems in academic research.

Controls are required for any plasmid-based experiment in order to accurately assess the results. This blog posts reviews different types of control plasmids, including but not limited to internal controls, negative controls, positive controls, and replicate controls.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Create a more sustainable lab by making small changes in your day-to-day or aim for long-term conservation goals. Find easy action items in the blog post.

A well-designed experiment relies on well-designed controls - so check out our post to learn all about these unsung heros of the experimental world!

An introduction to conflict resolution, identifying types of conflict and strategies for solving them.

Get a behind-the-scenes look at how we've adapted video production during COVID-19.

Learn how to place an order using a PO number for your Addgene order.

The Addictive Brain is a new platform to communicate science to broad audiences. Check out this blog post to learn more and for some science communication tips.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

The PUF Assembly Kit from Huimin Zhao's lab makes it possible to devise designer PUF proteins to hit any RNA target of interest.

Advice for things you can do to develop transferable skills while you are also learning to be an excellent scientist.

GreenGate is a cloning system for rapidly assembling plant transformation constructs and is based on the Golden Gate method.