... and should be designed accordingly. If you are trying activate a gene, you should design a control that...that shows maximal activation. Likewise if you are trying to repress a gene, your control might be a system...

...great place to start if you’re new to the topic. Or, try the search bar Now, you can search for the articles...

...protocols, and, of course, plasmids. Today we’re trying a new way to present plasmid info with a new segment...

...instead of incubating at -20°C for 1 hour, you can try incubating at -80°C overnight. Centrifuge for 20 ...

...your data, too!   Using the Data Hub If you're trying to decide which AAV is right for your experiment...

...protect the UV box from being cut by the razor blade. Try to get as little excess gel around the band as possible...

...taking a look at what works and what doesn’t, and trying all over again. Here’s that process!   Figure...Shot without anyone else in the office.    I try to build every setup with an eye to the future. I...

...to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment! APEX...favorite protein or RNA motif, give these methods a try.  References:  Branon, Tess C., et al. "Efficient...antibody based affinity purification and mass spectrometry approaches. Recently however, scientists have...classic antibody based affinity purification/mass spectrometry methods.  BioID: a promiscuous biotin ligase...purified using streptavidin and separated via flow cytometry.  Find plasmids for EXCELL here! Since BioID was...

...the world so it feeds a lot people. This lab is trying to improve homologous recombination efficiency ...injection into adult female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive...

... The PAM sequence is of particular concern when trying to edit a gene using homology directed repair, ...of reagents available through Addgene! If you're trying to decide which CRISPR tools are best for your ...

...lentiviral preps unless absolutely necessary. Many labs try to use freshly collected lentivirus for downstream...well to other cell types. To overcome this issue, try transducing with a range of dilutions of your lentiviral...detection kits that use detector cell lines, ELISA, fluorometry, or PCR-based approaches to detect even low levels...

... associations across different universities are trying to compile more comprehensive demographic information...beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career...

...addition to doing some of the quick things above, try joining or starting a sustainability project. This...

...online is a little trickier -- but at Addgene, we try to make the process as smooth and simple as possible...

... should also be able to understand what you are trying to convey without having to extensively use Google...

...Jennifer Doudna’s lab analyzed metagenomic datasets to try to determine whether simpler and maybe smaller Cas...

...Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves, she does...

... does have one special tip for anyone looking to try this at home in their own lab: be very careful with...

... knockout.  If knockout samples are unavailable, try substituting with knockdown approaches using siRNA...weak, then it is best to reassess the protocol and try to find a way to reduce, if not eliminate, the noise...W. “Controls for Immunocytochemistry.” Journal of Histochemistry & Cytochemistry, vol. 59, no. 1, ...al. “Controls for Immunohistochemistry.” Journal of Histochemistry & Cytochemistry, vol. 62, no. 10...that the target protein was measured on.    Flow cytometry In addition to the controls listed above, viability...compensation controls are recommended for flow cytometry. A viability control allows you to detect dead...fluorophore and are critical to analyze multiplex flow cytometry experiments. We hope that this blog post will ...

... to have as much context as possible. When I am trying to resolve a conflict between two different teams... of conflict resolution. In compromise, you are trying to come to a consensus that all conflicted parties...