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We narrowed to 957 results for: Cre

Showing: 121 - 140 of 957 results

  1. Hot Plasmids - October 2022

    Type
    Blog Post
    ...Two of their constructs are currently available as Cre-dependent, EF1ɑ-driven viral vectors (AAV1) - and...called JEDI-2P. Using a custom, high-throughput screening platform to apply saturating mutagenesis on 21... 1: Summary of the approach and outcomes of the screen for improved genetically-encoded voltage indicators... in-vivo imaging of voltage fluctuations. Image credit: Liu Z, et al. Cell. 2022.  Find JEDI-2p viral... Anti-c-Myc [9E10] page for more details. Image credit: Addgene.   Find anti-c-Myc antibody here! ...

  3. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

    Type
    Blog Post
    ...stochastic multicolor labeling, Brainbow used the Cre-loxP system to express one of the three fluorescent...Addgene #104110 - #104112) (Figure 2). We’ve also created constructs with containing chemical tags such as...

  4. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    ...added flanking loxP sites and a heat-shock inducible Cre to generate a self-excising cassette (SEC). To make...have used PCR-based screening to detect mutants, but this method limits screening to a few hundred worms... less than eight hours worth of hands-on time. Creating a modular, selection-based gene tagging approach...rare events difficult. Dickinson et al. sought to create a new and efficient selection-based CRISPR/Cas9...construct will serve as the repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The...knock-ins. These candidate animals can then be further screened based on their Roller phenotype. If SEC is inserted...separates the targeted gene from its promoter, creating a loss-of-function allele as well as a promoter-FP...

  5. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... Resources on the Addgene Blog Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist's View Interview...artificial intelligence (including a team that created a novel form of AI that can learn new tasks and...

  9. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

    Type
    Blog Post
    ...expression levels of certain genes. A popular method uses Cre recombinase on transgenic flox animals to introduce...light or noise should be avoided as this will also increase stress levels and affect behavior. A fact to consider... also to minimize the damage of tissue due to increased pressure of the added liquid. The volume should...editing/deletion using CRISPR. In some cases, a decrease of the expression efficiency can be observed after...

  10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...Fluorescent Proteins Control Protein Expression with Cre-lox Control Protein Activity with GFP Resources ...possible to create other photoswitchable kinases? The general design of psMEK1 also worked for creating three.... The Problem:  There is no common method for creating optically controlled kinases. The Solution: In...et al, the Lin lab present a generic design for creating photoswitchable kinases and use MEK1 as a model...often hard to decipher because it’s difficult to create short pulses of kinase activity. Zhou et al studied...feedback on endogenous MEK1/2 phosphorylation by creating a short pulse of psRaf1 activity. They did this...just 5 minutes post-Raf1 inactivation, and then decreases. Treatment of the cells with a PP1/PP2A inhibitor...

  11. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...modular system for packaging, secretion, and delivery of specific RNAs, such as Cre mRNA or Cas9 mRNA and sgRNA...for its small size. They were able to increase activity and decrease the gRNA size by intensively remodelling... engineered guide RNAs Yong-Sam Kim’s lab also created a miniature yet active Cas system starting with...

  12. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type
    Blog Post
    ...dual virus and the experimental approach. (B) CR-Cre mice demonstrate pain response due to blue light ...microcircuits. “In the longer term, this screening platform may help us to create high-performance sensors to enable...mechanical allodynia, a condition where a light touch creates a painful sensation. The dorsal horn of the spinal...virus expression in CR neurons of the spinal cord. Created with BioRender.com. Image from Haoyi Qiu.   ...indicators. Tian developed a video-based high-throughput screen for directed evolution of biosensors based on their... to engineer many types of biosensors.   This screening method allowed Tian to optimize a red‑shifted ... the improved voltage indicator coming from the screen to understand mouse cortical and hippocampal neural...

  13. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ... expression, and compatible with constitutive or Cre-dependent expression. These sensors have also been...binding sites per sensor (to decrease unnecessary calcium buffering and to increase sensitivity), and is a ...fundamental to muscle and nerve function, hormone secretion, blood clotting, and as we all know, a structural...illumination with violet light and (2) exposure to increased intracellular calcium. It has been applied to ...

  14. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Lenti-sgAtm#1/Cre ATM CMV Ataxia telangiectasia Charles Swanton 173580 Lenti-sgAtm#2/Cre ATM CMV Ataxia...Lewy body dementia Tiago Outeiro 89643 Lenti-sgAtm/Cre ATM U6 Ataxia telangiectasia Monte Winslow 89755 ...T5 Parkinson's Suzanne Pfeffer 208392 Lenti-sgRNA-Cre-GpNLuc-sgmATM ATM Ataxia telangiectasia Gaorav Gupta...Spinocerebellar ataxia 19 James Trimmer 129409 Slc1a3-CreERT2 Targeting Vector SLC1A3 PGK Episodic ataxia Walker...

  15. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ..., lentiviral, retroviral, AAV, RNAi, luciferase, cre/lox, yeast expression, worm expression, insect expression...and Organization where your plasmids were first created. From there, we’ll use the information in the ...

  16. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...light-dependent Gal4 transcriptional activation and Cre recombination. Find PhoCl plasmids at Addgene. Zhang... for easy removal of the resistance cassette via Cre recombination. Each knockout includes unique barcodes...natural ancestors), and screened through libraries of new RFP variants for increased fluorescence, brightness...polycistronic cassettes and discovered reprogramming discrepancies, which they traced to a 9 amino acid N-terminal... did not lead to the observed reprogramming discrepancies. The polycistronic piggyBac transposon vectors... protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics tools use light to alter...PMID: 28241009 CRISPR coselection with ouabain increases editing efficiency Blue flame depositor Yannick...

  18. Common Injection Routes in Mice

    Type
    Blog Post
    ...Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids References Prathipati, P., Rodriguez-Aguayo...representation of common injection routes in mice. Image created with biorender.com.   Needle techniques Intravenous... often used when injecting a cell suspension to create xenograft models. Restraint: back scruffing or ...

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...targeted loci. The selectable marker can be removed by Cre-mediated excision for scarless tag insertion.   In...lies in the ability of the endonuclease Cas9 to create a DNA double stranded break (DSB) at a genomic ...user wants to carry out a high-throughput genetic screen, it is expensive and time consuming to individually...each insertion. Also, it remains challenging to screen for the genetically modified strains that contain...simultaneously introduce a selection marker for screening modified strains. With SapTrap, the user first...selectable marker (floxed Cbr-unc-119) for easy screening of the insertion event, and a variety of connector...hundreds of genes, generating powerful genetic screening libraries. Additional protein tagging systems ...

  20. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...expression, shRNA-mediated knockdown, Tet and Cre-regulated expression, CRISPR genome editing, and more...requirement for the HIV Tat protein, thus decreasing the probability of creating replication-competent lentivirus...an extensive collection of lentiviral plasmids created for a variety of applications including cDNA expression... very common due to its broad host range, and increased particle stability; however, the protein is cytotoxic...replication-deficient meaning they can be used to create virus that is capable of infecting target cells...our eBook: Plasmids 101 Learn about genome-wide screening with CRISPR and Lentivirus Read about the ClonTracr...
Showing: 121 - 140 of 957 results