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Recent highlights from Addgene's collection include tools for epigenetic engineering, tracking cell-cell interactions, and a new auxin inducible degron system for C. elegans.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

Recent highlights from Addgene's collection include CRISPR-based editing in organoids and Biosensors for detecting strigolactones.

Learn more about Addgene's NGS verification process for pooled libraries using real-life amplication data from the Brunello gRNA library.

Recent highlights from Addgene's collection include tools to study SARS-CoV-2 antibody escape, antibodies for aminoacyl tRNA synthetases, and plasmids for generating nanocompartments for yeast bioproduction.

Check out some of the new and useful tools recently deposited in the Addgene repository!

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Summary of degron tags along with their applications, advantages, and several commonly used tags.

New fluorescence resonance energy transfer (FRET)-based calcium biosensors and FRET-based sensors for the intracellular imaging of transition metal ions.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

Learn how repressible promoters work and how you can use them in your next experiment.

Learn about the advantages of secondary nanobodies from the Gorlich lab over traditional antibodies. Applications include one-step fluorescent immunostaining.

In the second episode of our Hot plasmids podcast, learn about new RFPs, AAV tools, and plasmids for generating transgenic zebrafish.

Light-inducible transcriptional effectors (LITEs) have been designed by Feng Zhang's lab to bind specific genes and turn them on or off in response to light.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.