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We narrowed to 897 results for: plasmid

Showing: 121 - 140 of 897 results

  1. Hot Plasmids February 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  ...all applications. Addgene’s plasmid collection includes over 10,000 plasmids with a FLAG tag (!), but did... al. 2023 with permission.   Find Cas-PAGE plasmids here!   Zhang, Z., et al. (2023). Efficient engineering...inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando, R., et al. (2023). StayGold...under CC-BY license.   Find the AAV.CAP-Mac plasmid here!   Chuapoco, M.R., Flytzanis, N.C., Goeden...(2023) under CC-BY license.   Find AAV1-X1 plasmid here! Chen, X., et al. (2023). Functional gene ...

  2. Expanded Access to Rabies Plasmids

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    Blog Post
    ... ship rabies plasmids and viral vectors containing genetic elements from rabies plasmids to international... materials in our repository, including rabies plasmids, are export-controlled materials in the USA, where...export license applications for VSV and SARS-COV-1 plasmids. Export control license process    An export ...license, once approved, allows Addgene to ship plasmids or viral vectors containing sequences from rabies...  Timelines  We have offered VSV and SARS-COV-1 plasmids under this application process since May 2020....at [email protected].  Curious about how rabies plasmids are used in the lab? Check out our “Rabies and...browse tools for AAV tracing, made from rabies plasmids deposited by Ian Wickersham’s lab.   ...

  3. Hot Plasmids - November 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles. .... Today, it's time for another edition of Hot Plasmids!  Here's what you'll find in this post:  New...., 2023 under CC-BY 4.0 License. Find PE6 plasmids here!  Doman, J.L., Pandey, S., et al. (2023)....therapies and medical applications.  Find DIRECTED plasmids here! Strebinger, D., et al. (2023). Cell type-specific...pulse-chase experiments, and more. Find LOV-Turbo plasmids here! Next, Qin and Cheah et al. described TransitID...LOV-Turbo into TransitID experiments.  Find TransitID plasmids here! These two developments open up new possibilities... promoter activity in single cells. Find iPAK4 plasmids here! Lin D., Li X., et al. (2023). Time-tagged...

  4. Plasmids 101: Gateway Cloning

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    Blog Post
    ...to find Gateway plasmids for your organisms of interest: Find Gateway cloning plasmids here! References...antibiotic resistance markers (indicated here by plasmid color), allowing you to easily select for the expression...desired, recombined construct. Read our recent Plasmids 101 Post on CcdB for more information. How to ...Method A: recombination of an attB-PCR product or plasmid with an attP donor vector. In this case, we would... to use the Snapgene program to design Gateway plasmids.    Figure 3: Method A to create an entry...entry clones can be used with a large variety of plasmids. Speed The Gateway system enables the generation.... Take a look at some of the Gateway Multisite plasmids available at Addgene, including the Frew Lab Multiple...

  5. Plasmids 101: TOPO Cloning

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    Blog Post
    ...Deletions in a Plasmids Learn How to Verify Your Plasmid Resources on Addgene.org Find More Plasmid Cloning...

  6. Plasmids 101: Inducible Promoters

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    Blog Post
    ...Resources on the Addgene Blog: Plasmids 101: The Promoter Region Plasmids 101: Cre-lox Light-Switchable...Promoter Subtype Promoter Example Activator Addgene Plasmid/Kit Alcohol-regulated AlcA promoter AlcR pGGA008...use light to regulate transcription. Red flame plasmid pDawn contains the blue-light sensing protein YFI...Systems for E. coli Browse other blog posts from our Plasmids 101 series Resources on Addgene.org: Inducible...Inducible Systems at Addgene Cre-lox Guide and Plasmids     ...

  7. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...modifications Learn more molecular biology in our Plasmids 101 eBook! Researchers use various techniques ...for your protein of interest is inserted into a plasmid expression vector that is then transformed into...being able to help the scientific community share plasmids.   Additional Resources Thermofisher Protein ...Addgene.org Bacterial expression systems Search for plasmids by expression system ...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles.  ...2 nucleocapsid protein (Plasmid #141391-LV [+N]) or membrane protein (Plasmid #141386-LV [+M], negative... over the original CROPseq. Find CROPseq-multi plasmids here Walton, R.T., Qin, Y., Blainey, P.C. (2024...potential improvements to the editor. Find PE7 plasmids here!     Figure 2: PE7 improves prime...conjugated with its HaloTag domain.  Find Voltron2 plasmids here! Abdelfattah, A.S., et al. (2023) Neuron...therapeutic applications.  Find microglia-targeting AAV plasmids here! Serrano, C., et al. (2023). Simple and ...potential for therapeutic success. Find REVeRT plasmids here! Riedmayr, L.M., et al. (2023). mRNA trans-splicing...

  9. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...on Addgene.org CRISPR Plasmids – Protein Tagging CRISPaint Gene Tagging Plasmids 101 eBook Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Plasmids for Endogenous Gene Tagging in Human Cells ...cloned in-frame to the protein of interest for plasmid-based expression systems. Degron tags are one of...

  10. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...As a plasmid repository, we are no strangers to cloning. If you’re reading this, then chances are you...NEB’s DAD Interview with Nicola Patron on MoClo Plasmids 101: Golden Gate Cloning ...

  11. Hot Plasmids - January 2023

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ... missed something smokin'? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ...Figure 3 Colognori et al 2022.   Find CRISPR Csm plasmids here! Colognori D, Trinidad M, Doudna JA. Precise...inducible expression.   Find heat-inducible lentiviral plasmids here!   Wu Y, et. al. Control of the activity ...2: Addgene’s western blot for mEGFP-HRas (from Plasmid 18662). See the Applications section on the Anti-GFP... Campbell et al, Nature Methods   Find YFP plasmids here!   Campbell, B.C., et al. Chemically stable...

  12. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    ...Tet-Off system.  For more information on Addgene Tet plasmids, see our Tetracycline resource page. Cumate Switch...20697123. PubMed Central PMCID: PMC2942869. Find plasmids from this publication at Addgene. del Valle Rodriguez...20434990. PubMed Central PMCID: PMC2883883. Find plasmids from this publication at Addgene. Wei, Xing, ...22406855. PubMed Central PMCID: PMC3846601. Find plasmids from this publication at Addgene. Riabinina, ...25581800. PubMed Central PMCID: PMC4344399. Find plasmids from this publication at Addgene. Riabinina O...Hill, C. et al. A LexAop > UAS > QUAS trimeric plasmid to generate inducible and interconvertible Drosophila...

  13. Hot Plasmids - October 2022

    Type
    Blog Post
    ...of the new plasmids, antibodies, and viral preps in the repository through our hot plasmids articles. ...Think we missed something hot? You can pitch a Hot Plasmids section (antibodies and viral preps welcome!) ... you can also find several other constructs in plasmid form. Fig. 1: Summary of the approach and...JEDI-2p viral preps here (and here!)Find JEDI-2p plasmids here!  Liu, Zhuohe et al. Sustained deep-tissue... improve the performance of HcKCRs. Find HcKCR plasmids here!   Govorunova EG, et al. Kalium channelrhodopsins...’ in the Addgene catalog pulled up over 13,000 plasmids! Our recombinant anti-c-Myc [9E10] (isotype IgG2a...myc-tagged protein GFP-smFP-myc expressed from Plasmid 98926. See the Applications section on the Anti-c-Myc...

  14. Hot Plasmids - May 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here. Here's what you'...easily be applied to other systems.    Find BLITZ Plasmids here! Xiong, et al. eLife. 2021. https://doi.org...from Goertsen D, et al., 2022.  Find M-CREATE plasmids here!  Goertsen D, et al. Nature Neuroscience....have higher risks of off-target mutations. These plasmids contain catalytically inactive dCas9 and microbial...Image from Wang, et al, 2022.  Find dCas9-SSAP plasmids here! Wang et al. Nat Cell Biol. 2022. https:/...

  15. Hot Plasmids - February 2022

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'...Ladder  by: Rachel Leeson This simple, inexpensive plasmid-based ladder system is a cost-effective alternative... ladders. Image from Santilli, et al. 2021 Plasmids are used to express 10, 15, 20, 30, 40, 50, 60...kD proteins or 20, 40, 60, and 80 kD proteins. Plasmids containing 150 or 250 kD proteins are also available...

  16. Hot Plasmids - October 2020

    Type
    Blog Post
    ...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...highlights from recent CRISPR plasmids. To find all of the CRISPR plasmids available from Addgene, head...need. If you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll...head over to our CRISPR Plasmids and Resources page.  CasPhi is 70 kDa Cas protein identified exclusively...offers advantages for cellular delivery. Get the plasmids at Addgene Sc++ is a codon-optimized, engineered...nuclease that has a broad 5′-NNG-3′ PAM. Get the plasmids at Addgene The Human GlycoGene CRISPR Library...

  17. Plasmids 101: Colony PCR

    Type
    Blog Post
    ...the correct orientation or even if it’s in your plasmid backbone. Backbone-specific primers: A second...orientation of your insert or if the insert is in your plasmid Have to make a new primer pair for each insert ...about the size of the insert and if it's in the plasmid Can be used to screen clones that were created ...created with the same backbone because primers are plasmid dependent Doesn't provide information about the...insert Tells you if the insert is present in the plasmid Great for blunt end cloning You have to make...thermocycling program. One key difference is the plasmid DNA must be released from the bacteria in order... just need to lyse the bacteria to release the plasmid DNA by either briefly boiling the sample before...

  18. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...In a previous post from our Plasmids 101 series, we learned how the Cre-loxP recombination system can... even in the presence of Cre recombinase. This plasmid now specifically drives expression of mCherry instead...2286602. PubMed Central PMCID: PMC3406316. Find FLEx plasmids from these Addgene depositors: Ed Boyden Bernardo... plasmids... plasmid... plasmids 101... flex plasmid...

  19. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...previous posts for our Plasmids 101 series, we examined a number of important plasmid elements – promoters...Resources on Addgene.org Find Cre plasmids Find loxP plasmids  ... expression proceeds. One popular lox-stop-lox plasmid is from Tyler Jack's lab: Lox-Stop-Lox TOPO.  Cre-dependent...  plasmids 101...

  20. Plasmids 101: Protein tags

    Type
    Blog Post
    ...localization, or protease protection. Thus far Plasmids 101 has covered GFP and its related fluorescent...stability activity, can be readily purified using plasmids found in this paper (available at Addgene).   ...interest: Which Fluorescent Protein Should I Use? Plasmids 101: Green Fluorescent Protein (GFP) Fluorescent...   plasmids 101...
Showing: 121 - 140 of 897 results