We narrowed to 222 results for: ung

1. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

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   ...Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing insurance, finance and high-tech ...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

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   ...Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: ...

3. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

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   ...CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...

4. 3 Challenges in Plant Synthetic Biology

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   Blog Post

   ... However, the rewards are great if you take the plunge – plants are the foundation of life on earth, and...

5. CRISPR Plasmids - Zebrafish

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   ... In vitro transcription none, need Cas9 plasmid Joung Do you have suggestions for other plasmids that ...

6. Addgene: The First Twenty Years

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   ...Yay4sean, in r/molecularbiology.   Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production...

7. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...localization of hM4Di in monkeys. The pre-embedding immungold technique was used to reveal the hM4Di coupled...

8. RNA Extraction Without A Kit

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   ...RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work quickly...

9. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

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   ...PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization...

10. New Tool for Lineage Tracing: The ClonTracer Library

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    ...to drug treatment. They infected a non-small cell lung cancer cell line containing an activating mutation...

11. CRISPR Plasmids - C. elegans

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    ... In vitro transcription none, need Cas9 plasmid Joung 46170 PU6::klp-12_sgRNA cU6 PCR (see paper) Transfection...

12. Plasmids 101: The Wide World of Natural Plasmids

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    ...plasmid can grow in spite of a hostile Penicillium fungus trying to kill them off. While antibiotic resistance-mediated...

13. Hot Plasmids February 2024

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    ...delivering a transgene, with neuron bias observed in young macaques and vascular bias in mature marmosets. ...

14. Viral Vectors 101: Chemogenetics

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    ...Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019) Olanzapine: A potent...

15. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3...

16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    ...genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their...

17. Hot Plasmids and Viral Preps - March 2021

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    ... markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver’s labs are now available...

18. Fluorescent Proteins 101: Introduction to FRET

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    ...Kapanidis, Achillefs N. "Taking the ruler to the jungle: Single-molecule FRET for understanding biomolecular...

19. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

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    ...nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...

20. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

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    ...Murray R, Packer MS, Rinaldi C, Slaymaker IM, Yen J, Young LE, Ciaramella G (2020) Directed evolution of adenine...