Advice for Scientists Starting a Lab
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...emailing us his worldly advice”, the other was “a young PI who was in the lab with us every day and in the...
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Cre-Lox and Other Site-Specific Recombinases
TypeCollection...recombinase (SSR) systems, like Cre-lox, derive from fungi or bacteriophages and are powerful genome engineering... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...Make scicomm practice a priority The good news for young scientists is that we all build and develop skill... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog Post... are ready to take (if you haven’t already) the plunge into the world of precision genome editing. When... -
Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors
TypeBlog Post...Health Plan, Carl worked at both MetLife & Ernst & Young, LLP, auditing insurance, finance and high-tech ... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog Post...on the shoulders of giants. The still relatively young movement of sharing open hardware has a similar ... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog Post...Foden JA, Thapar V, Reyon D, Goodwin MJ, Aryee MJ, Joung JK. Nat Biotechnol. 2014 Jun;32(6):569-76. doi: ... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog Post...CRISPR-Cas9 complex. Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post... However, the rewards are great if you take the plunge – plants are the foundation of life on earth, and... -
Addgene: The First Twenty Years
TypeBlog Post...Yay4sean, in r/molecularbiology. Oh, to be young again! Addgenie Marcy (now our Sr. Director of Production... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog Post...PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization... -
RNA Extraction Without A Kit
TypeBlog Post...RNaseZAP. When harvesting tissues, cells, plants, fungi, or bacteria, keep samples cold and work quickly... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post...localization of hM4Di in monkeys. The pre-embedding immungold technique was used to reveal the hM4Di coupled... -
New Tool for Lineage Tracing: The ClonTracer Library
TypeBlog Post...to drug treatment. They infected a non-small cell lung cancer cell line containing an activating mutation... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog Post...plasmid can grow in spite of a hostile Penicillium fungus trying to kill them off. While antibiotic resistance-mediated... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...CRISPR Guide to Epigenetics References Charlton J, Jung EJ, Mattei AL et al. (2020) TETs compete with DNMT3... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...genes are important for SARS-CoV-2 entry into human lung epithelial cells (Biering et al., 2021). In their... -
Hot Plasmids February 2024
TypeBlog Post...delivering a transgene, with neuron bias observed in young macaques and vascular bias in mature marmosets. ... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019) Olanzapine: A potent... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog Post...nmeth.3871 Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg...