We narrowed to 224 results for: antibody

1. SARS-CoV-2 Pseudotyped Virus

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   ...Addgene Blog Posts Viral Vectors 101: Pseudotyping Antibody Neutralization Response Against Pseudoviruses ...

2. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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   ...sequences are highly homologous, preventing design of antibodies specific to each fluorophore. Third, Brainbow...mKate2.) They then successfully generated custom antibodies to each of these proteins and confirmed that ...

3. CRISPR Plasmids - Yeast

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   ..., and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design ...

4. New England Biolabs Cell-Imaging Plasmid Collection

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   ...secreted proteins with disulfide bridges such as antibodies. MCP-tag is a mutant of ACP-tag, and is labeled...

5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

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   ... to easily identify your protein using an antibody against that epitope. This could allow you... your favorite gene even if you do not have an antibody against it. Another common scenario is fusing...

6. 28 Hot Plasmid Technologies from 2015

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   ...Multimerization in the SunTag system occurs via antibody-peptide labeling; specifically, cognate peptide...recognized and thus fluorescently labeled by scFv antibodies fused to sfGFP. This system amplifies the intensity...scaffold containing epitopes recognized by scFV antibodies fused to VP64 transcriptional activation domains...more - all without the need to develop custom antibodies. Unfortunately, while there are many types of...proteins, ordered structures, and accessibility to an antibody. After identifying possible protein domains containing...containing these properties, they generated monoclonal antibodies against them, fused them to GFP, and compared...

7. Exploring Applications of the Bioluminescent HiBiT Tag

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   ...native protein context, but they still depend on antibody-based detection or western blotting, methods that... degradation, trafficking (Hall et al., 2012). Antibody-based tools can also be combined with the HiBiT...

8. Technique: Probe Phage Genomes for Host Binding Proteins

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   ...characterization, these proteins could function as surrogate antibodies for identifying and characterizing sugars and...temperature. If you’re lucky enough to have an antibody or other protein on hand that will specifically...

9. Science Guides

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   ... below. Antibodies Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced...system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based applications... applications, which often use antibodies conjugated to a signaling molecule, can be used to capture, ...

10. Promega Plasmid Collection

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    ...bioluminescent assays or with the high-affinity anti-HiBiT antibody for additional detection options. HiBiT technology's...

11. SunTag and Fluorescent Imaging

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    ...GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. This...

12. London Calling - Addgene’s New Europe Office in London

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    ...others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These genetic tools...

13. Running for Rare Disease, Running for FOP, Running for AJ

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    ...Activin A. A commercially developed therapeutic antibody that binds Activin A and interferes with inappropriate...

14. Choosing Your Perfect Empty Backbone

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    Blog Post

    ... or purify it – and you do not have a specific antibody against the protein available – you can always...

15. Addgene's eBook Collection

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    Guide

    ... Overlay Select Antibodies 101 New! October 2024 | First Edition About Antibodies 101 October 2024...contains practical introductions and helpful tips for antibody-based applications, from flow to IHC! Close About...

16. CRISPR Plasmids - Bacteria

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    ..., and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. Design ...

17. Visualizing Translation at the Single Molecule Level

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    ...that can recruit GFP fused to the SunTag-specific antibody scFv. Each time the reporter is translated, the...

18. In Vivo Biotinylation of Bacterial Fusion Proteins

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    ... rabbit (EMD, AB3596) and mouse (NEB, E8032S) antibodies against MBP are commercially available. Factor...

19. CRISPR Plasmids - Mammalian Expression

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    ..., and DNA. The enCHIP system uses an anti-FLAG antibody to immunoprecipitate FLAG-tagged Cas9. In the ...

20. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

    ...15 kDa), single monomeric variable heavy chain antibody domain.  How do these components interact? When...