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Showing: 161 - 180 of 960 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...induced pluripotent stem cells (iPSCs) capable of forming nearly all cell types in the body from otherwise... battery of questions regarding topics such as informed consent (How do we prevent stem cell lines from...meeting shared plenty of details, but they also uniformly stepped back to give a big picture perspective...

  2. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...medium, without which the cells cannot reproduce to form colonies (used to complement auxotrophic cell lines...produces pigment from x-gal (included in the transformation plate medium), turning the bacterial colony...for blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies...components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may contain genes that...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...revision before acceptance. F1000Research uses a novel form of peer review that occurs post-publication and ... required information is provided. The clearer you write your paper and the more information you provide...journal’s coverage. These guidelines also provide information on a journal’s house style and the things you...you need to include in your manuscript. What information do I need to include in my paper? More and more...more journals are asking for detailed information on lab resources, such as catalogue numbers and manufacturers...joined a pilot project from the Neuroscience Information Framework (NIF) called the Resource Identification...whether a manuscript contains all the necessary information to be of maximal use to the scientific communities...

  4. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...directly into the genome to form a stable transfection. Unlike bacterial transformation, scientsts do not "select...Transfection is somewhat comparable to bacterial transformation (the introduction of DNA into bacterial cells...efficient selection. This is usually achieved by performing a "kill curve" (basically growing cells in various...

  5. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...a fusion construct or by co-transfection. HMCES forms a chemical crosslink with DNA abasic sites, protecting... DNA for Cas9 to bind.   R-loop The structure formed by Cas9 binding to DNA and separating the two strands...hypothesized that the adenosine deaminase could also perform cytidine (C) deamination, converting the C base...uracil (U). This is the same chemical reaction performed by CBEs. However, most CBEs include an inhibitor...

  6. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...successfully cloned into this vector, transformants will instead form white colonies. Adenoviral Vector ID...process as it can be used in blue-white screening and forms blue colonies on LB/Amp/Xgal/IPTG plates — if your...pAdx-CMV-LacZ 73345 Expresses lacZ from the CMV promoter, forms blue colonies on LB/Amp/Xgal/IPTG plate pAdx-CMV-copGFP...

  7. All Antibodies

    Type
    Collection
    ... to the collection using our Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody ...Antibody Plasmids , fill out our Suggest an Antibody form, or email [email protected] to help us improve our...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...combination of metabolomics and genome engineering forms the core of the deorphanizing engine. Genetic and...Partnership using their RESOLUTE repository feedback form . If you have further questions or comments you ...

  11. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ... colonies are called satellite colonies and they form when all the antibiotic has been used. Don’t worry...the specific reaction you are running. Transformation Transform a few microliters of your ligation reaction...The PCR could fail to produce a product, the transformation may not result in any cells, or all the colonies...primer to 40-60 bp. It is always a good idea to perform primer optimization, especially if you are having...fragments If you don’t get any positive clone, try transforming the ligation mix in different bacterial strains...

  13. A Quick Guide to a Career in Software Product Management

    Type
    Blog Post
    ...product managers collate and organize that information and form it into a roadmap--a plan for future features... needed to know information from the lab folks, it was my job to get it into a format that helped the ...programs, or tools to build. After organizing the information, a software product manager works with software...translate complex lab operations workflows into a format the developers could understand, so they could ... Then, I distill and translate that valuable information back to the rest of my team in Oxford (UK) to...frameworks to use. I mostly sit and listen to stay informed on the details, but the developers have it under...

  14. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...base editors that can be reconstituted in vivo to form a protein identical in sequence to the unmodified...promoters, CRISPRi parts, selectable markers, transformation acceptor vectors, and sequences for genomic...plasmids using the Cyanogate Kit and subsequent transformation into cyanobacteria. Image from Vasudevan et...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...components of the virus are now delivered in other forms (mRNA, DNA, etc.) to raise immunity. Viral vectors...PMC2075523. Bulcha, J.T., et al. Viral vector platforms within the gene therapy landscape. Sig Transduct...

  16. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...“chewed back” overhangs, they can be annealed, forming a circular product with four nicks easily repairable...strong enough to hold a plasmid together through transformation, so the cloned vector must be ligated. But ...repairable by the bacteria after transformation. LIC is a reliable cloning method, but it is limited by its...recombination intermediate to bacteria, so it can be transformed as-is into cells. The cells then get to work ...is long enough to hold it together, it can be transformed into bacteria, which will efficiently repair ... annealed. The recombination intermediate is transformed into bacteria, which repair the plasmid. Created... To overcome this limitation, one option is to perform a hierarchical assembly, assembling fragments in...

  17. Clear and Accessible Writing

    Type
    Blog Post
    ... focus on practicing with a short and accessible form of writing: alt text (pun intended.)  Steps to clear...present the image and information. Where is the reader accessing the information? This can be physical...readers to provide visually impaired visitors with information about images that a sighted reader would glean...were many barriers between our audience and the information in the paper.  Of course, when you think about...helps visually impaired readers receive the same information as sighted readers.    Figure 1: An image... or digital. For instance, you would present information differently in a poster compared to an online... when writing your alt text, use all of this information to describe the image helpfully. You and your...

  18. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    ...variants of interest, though. A selection can take the form of varied temperature, nutrient availability, drugs...treat cells with many different drugs and then perform an assay to look at an outcome. Your scientific...capacity. Drug screens, on the other hand, are often performed as biased forward screens. A given panel of drugs...reverse screens, except that they begin with a uniform population possessing a specific mutation or phenotype...phenotype. For example, a drug screen may be performed on a cell line possessing a patient mutation to...
Showing: 161 - 180 of 960 results