A Control for All Seasons
Type
Blog Post
...groups with generally sticky proteins such as bovine serum albumin.
Secondary antibodies can also react with...preimmune serum as well as with an isotype-matched control.
Figure 3: A preimmune serum control...specificity is further supported by a pre-immune serum or isotype-specific control (Hewitt 2014, Alexander...the primary antibody is replaced with pre-immune serum from the animal the antibody was produced in or ...nonspecifically through the Fc region (orange). The Preimmune serum control will not bind to the desired target but ...