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Showing: 1 - 13 of 13 results
  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Updated
    Oct. 13, 2019, 10:05 p.m.
    ...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...activity by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation...similarity. For these three kinases, photoswitchable Dronpa domains were inserted at sites homologous to the...here. In addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et...proteins could potentially be created by inserting pdDronpa domains into loops of a protein of interest. If...
  2. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    Updated
    Oct. 13, 2019, 10:01 p.m.
    ... make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to oligomerize when...To do so, she generated fusion proteins in which Dronpa domains flanked either side of an enzyme’s active...ultraviolet light exposure caused oligomerization of the Dronpa domains thereby occluding the enzyme active site...the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back on....optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase its...iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin has been.... She additionally showed that these enhanced “pdDronpa” domains could be placed in amino acid loops on...
  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Updated
    Feb. 10, 2021, 1:37 p.m.
    ...both activates and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less...known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing...vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing...chromophore, leading to a non-fluorescent state. Dronpa can be reversibly reactivated by exposure to UV...scientists used mutagenesis screens to find a version of Dronpa that doesn't become inactivated as quickly. Padron...monomeric, reversible PA-FP derived from mutating Dronpa (T59M / V60A / N94I / P141L / G155S / V157G / M159Y...positive” switching behavior is the opposite of Dronpa; when exposed to blue light, Padron is photoconverted...
  4. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Updated
    June 12, 2020, 3:56 p.m.
    ...interactions with photoactivatable fluorescent protein Dronpa. The authors tracked protein kinase A (PKA) activity...living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate fused to the phosphopeptide...a conformational change that placesTagRFP-T and Dronpa in close proximity. By monitoring changes in TagRFP-T...
  5. Michael Davidson Fluorescent Protein Collection

    Type
    Collection
    ...Supplement Dronpa-Peroxisomes-2 Localization: Peroxisomes, Excitation: 503, Emission: 518 Dronpa-Peroxisomes...Data Supplement Dronpa-Lifeact-7 Localization: Actin, Excitation: 503, Emission: 518 Dronpa-Lifeact-7 Data...Data Supplement Dronpa-Actin-2 Localization: Actin, Excitation: 503, Emission: 518 Dronpa-Actin-2 Data Supplement... Supplement Dronpa-Actin-7 Localization: Actin, Excitation: 503, Emission: 518 Dronpa-Actin-7 Data Supplement...Supplement Dronpa-Actin-C-18 Localization: Actin, Excitation: 503, Emission: 518 Dronpa-Actin-C-18 Data...Supplement Dronpa-Alpha-Actinin-19 Localization: Alpha Actinin, Excitation: 503, Emission: 518 Dronpa-Alpha-Actinin...503, Emission: 518 Dronpa-Alpha-V-Integrin-25 Data Supplement Dronpa-Beta-Catenin-20 Localization: Tight...
  6. Optogenetics Guide

    Type
    Guide
    ... 470 Fluorescent Protein domains Dronpa based Light-inducible Dronpa mutant domains that associate and... the applications described above, both LOV and Dronpa can control activity of a fused protein through...
  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Updated
    April 27, 2021, 2:59 p.m.
    ...dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa dimers dissociate in response to... to cyan light (500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft...binding in the dark. Upon light stimulation, the pdDronpa moieties move apart, allowing DNA binding and ...Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent...
  8. Plasmids for Optogenetics Research

    Type
    Collection
    ...41640 pcDNA3-K-I-N-CAAX Dronpa145N Mammalian Lin 41642 pcDNA3-K-I-CAAX Dronpa145N Mammalian Lin 41643 pcDNA3...pcDNA3-I-K-CAAX Dronpa145N Mammalian Lin 41644 pcDNA3-K-CAAX Dronpa145N Mammalian Lin 41645 pcDNA3-mNeptune2...mNeptune2-N Dronpa145N Mammalian mNeptune2 Lin 42214 pGL3-Basic-9xSeq2-Luc LITEZ Mammalian Gersbach 42215...
  9. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...hGRIN2B-CAG-ps-SpCas9 sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (Homo sapiens) Mammalian Expression, CRISPR Lin...hGRIN2B-CAG-ps-SaCas9 sa-hGRIN2B-sgRNA; dSaCas9; pdDronpa1 (Homo sapiens) Mammalian Expression, CRISPR Lin...
Showing: 1 - 13 of 13 results