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We narrowed to 7 results for: Dronpa

Showing: 1 - 7 of 7 results
  1. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    ...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...activity by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation...similarity. For these three kinases, photoswitchable Dronpa domains were inserted at sites homologous to the...
  2. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

    Type
    Blog Post
    ... make enzymes controllable by tethering them to Dronpa, a fluorescent protein known to oligomerize when...To do so, she generated fusion proteins in which Dronpa domains flanked either side of an enzyme’s active...ultraviolet light exposure caused oligomerization of the Dronpa domains thereby occluding the enzyme active site...the enzyme. Later exposure to cyan light stopped Dronpa oligomerization and turned the enzymes back on....optimized these first generation FLiPs by mutating Dronpa to lower its aggregation tendency, increase its...iterations of FLiPs only used N- and C-terminal dronpa fusions. With these various designs, Xin has been...
  3. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    ...both activates and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less...known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing...vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing...chromophore, leading to a non-fluorescent state. Dronpa can be reversibly reactivated by exposure to UV...scientists used mutagenesis screens to find a version of Dronpa that doesn't become inactivated as quickly. Padron...monomeric, reversible PA-FP derived from mutating Dronpa (T59M / V60A / N94I / P141L / G155S / V157G / M159Y...positive” switching behavior is the opposite of Dronpa; when exposed to blue light, Padron is photoconverted...
  4. Optogenetics Guide

    Type
    Guide
    ... Name Description Peak excitation λ (nm) Dronpa Mutant Dronpa domains associate in the dark, inhibiting...
Showing: 1 - 7 of 7 results