Lighting Up Cell Signaling with Photoswitchable Kinases
Type
Blog Post
...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...activity by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation...similarity. For these three kinases, photoswitchable Dronpa domains were inserted at sites homologous to the...here. In addition, it could be possible to use pdDronpa to control other kinases and proteins. Zhou et...proteins could potentially be created by inserting pdDronpa domains into loops of a protein of interest. If...