...cyan light, Dronpa dimers dissociate, Dronpa goes dark, and MEK1 is active or “on.” Dronpa’s color thus... the Dronpa dimers interact and lock MEK1 in an “off” setting by blocking the active site. Dronpa also...Additionally, dimeric Dronpa is less prone to aggregation in cells compared to the tetrameric Dronpa it was derived...activity by attaching two dimerizing photoswitchable Dronpa (dron= a ninja term for vanishing, and pa= photoactivation...similarity. For these three kinases, photoswitchable Dronpa domains were inserted at sites homologous to the...pulse of 500 nm light (turn on psRaf1) followed by 3 seconds of 400 nm light (turn off psRaf1). After this...is responsible for this MEK1/2 negative feedback. 3. Studying signaling pathways in vivo Expressing photoswitchable...

...both activates and inactivates Dronpa is not ideal, since this means Dronpa is quickly inactivated and less...known reversible PA-FP is the naturally occurring Dronpa protein (named after dron, a ninja term for vanishing...vanishing, and pa, for photoactivation) (6). Dronpa is photoactivated by blue wavelengths of light, causing...chromophore, leading to a non-fluorescent state. Dronpa can be reversibly reactivated by exposure to UV...scientists used mutagenesis screens to find a version of Dronpa that doesn't become inactivated as quickly. Padron...monomeric, reversible PA-FP derived from mutating Dronpa (T59M / V60A / N94I / P141L / G155S / V157G / M159Y...positive” switching behavior is the opposite of Dronpa; when exposed to blue light, Padron is photoconverted...

... 470 Fluorescent Protein domains Dronpa based Light-inducible Dronpa mutant domains that associate and... the applications described above, both LOV and Dronpa can control activity of a fused protein through...Halorhodopsin plasmids . Archaerhodopsins Archaerhodopsin-3 (Arch) from Halorubrum sodomense is also commonly ...with single-spike temporal resolution. PLoS One. 2(3):e299. PMID 17375185 Kim B, Lin MZ. 2013. Optobiology...excitation of distinct neural populations. Nat Methods. 11(3):338-46. PMID 24509633 Lin, J. 2011. A User’s Guide...control of neural activity. Cold Spring Harb Protoc. 3:top102. PMID 21363959 Yizhar O, Fenno LE, Prigge M...derived from Volvox carteri. Nat Neurosci. 11(6):631-3. PMID 18432196...

...pBAD-LSSmOrange - Bacterial Expression mKeima Red 440 620 3 6.5 4.4 hr Monomer pFA6a-link-yomKeima-CaURA3 - Yeast...dKeima-Red-N1 - Mammalian Expression LSSmKate1 463 624 3 3.2 1.7 hr Monomer pLSSmKate1-N1 - Mammalian Expression...HisB-PAmKate - Bacterial Expression PAiRFP1 690 717 3 Dimer pPAiRFP1-N1 - Mammalian Expression pBAD/HisB-PAiRFP1...HisB-PAiRFP1 - Bacterial Expression PAiRFP2 692 719 3 Dimer pPAiRFP2-N1 - Mammalian Expression pBAD/HisB-PAiRFP2... al. : Nature Communications, November 2012, Vol. 3 No. 1204 Wiedenmann et al. : Journal of Biomedical...Bacterial Expression Dronpa3 503 518 19 Monomer Dronpa3-N1 - Mammalian Expression Dronpa3-C1 - Mammalian Expression...

... expanded version by Nyla Naim was published Sept 3, 2020.   Scientists around the world have been making...dimeric green fluorescent protein (pdDronpa) (Zhou et al., 2018). pdDronpa dimers dissociate in response to... to cyan light (500nm). By fusing the two pdDronpa domains to the sites flanking the DNA-binding cleft...binding in the dark. Upon light stimulation, the pdDronpa moieties move apart, allowing DNA binding and ...Cas9, dCas9 Gene editing Zhou et al., 2018   pdDronpa Cas9, dCas9 Gene editing, transcription Light-Dependent...

...Parkinson's, FTD Michael Davidson 57286 Dronpa-MAPTau-N-10 MAPT Dronpa CMV Parkinson's, FTD Michael Davidson...TLS 2: hTLS.pCDNA1 FUS CMV ALS David Ron 21829 TLS 3: TLS.pRSET.C FUS His T7 ALS David Ron 21830 TLS 4:... APEX2 CMV Parkinson's Mark Cookson 165107 pGEX4T-3-6AOptParkin PRKN GST tac Parkinson's Arthur Haas 166671...dementia (FTD) Thomas Kukar 176922 pcDNA3.1(+)_Granulin 3+linker4 GRN Flag, Strep CMV Frontotemporal dementia...dementia (FTD) Thomas Kukar 176923 pcDNA3.1(+)_Granulin 3-no linker GRN Flag, Strep CMV Frontotemporal dementia...