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We narrowed to 5 results for: RLuc8

Showing: 1 - 5 of 5 results

  1. Understanding GPCR Signaling Using TRUPATH

    Type
    Blog Post
    ...was developed by constructing 14 luciferase donor Rluc8-Gα chimeras, four acceptor GFP2-Gγ chimeras, and...protein. For the Gα constructs, localization of the Rluc8 was more challenging. The Roth lab used structure-guided...refinement to determine the optimal localization of the Rluc8 insertion within the Gα. Despite exhaustive refinement...refinement, it was not possible to generate an optimal Rluc8-Gα construct for two of the 16 non-visual G protein... plasmids expressing the GPCR of interest, a Gα-Rluc8 plasmid, and the appropriate Gγ-GFP2 and Gβ constructs...inactive state, the luminescent chimeric protein Gα-Rluc8 and Gγ-GFP2 will be in close association, resulting...BRET2 ratio, which is the ratio of GFP2 emission to RLuc8 emission (see figure). What can TRUPATH be used ...

  2. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... applied using RLuc8, an engineered Renilla luciferase, to produce a series of FP-RLuc8 fusions for multicolor...fluorescent signal and these FP-RLuc8 fusions were dubbed Nano-Lanterns. RLuc8 is 5.5-fold brighter than RLuc...30-fold brighter than RLuc8 (6). Susuki, K, et al. therefore replaced the RLuc8 moieties in their Nano-Lanterns...

  3. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ..., a fusion of a mutagenized Renilla luciferase (RLuc8) with Venus. This luminescent technology is based...coelenterazine is supplied in the culture medium to allow RLuc8 to produce photons. The original nano-lantern emitted...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression Renilla luciferase mutant Rluc8, which has increased...

  5. Chemogenetics Guide

    Type
    Guide
    ...activation Slaviero et al., 2024 iLMO: RLuc-NpHR Venus-Rluc8 NpHR hCTZ Cl - influx Neuronal inhibition Tung et...
Showing: 1 - 5 of 5 results