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We narrowed to 109 results for: chm

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Showing: 1 - 20 of 109 results

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...pLNCX2-mEGFP-CHMP2B CHMP2B GFP CMV ALS Sanford Simon 115331 pLNCX2-mCherry-CHMP2B CHMP2B mCherry CMV ALS...-2A-CHMP2B CHMP2B mTagBFP2 CMV ALS Franz-Ulrich Hartl 232001 pCMV mTagBFP2-2A-CHMP2B-Q165X CHMP2B mTagBFP2...232002 pCMV CHMP2B-3XHA CHMP2B HA CMV ALS Franz-Ulrich Hartl 232003 pCMV CHMP2B-L4D F5D-3XHA CHMP2B HA CMV ...FLAG-CHMP2B CHMP2B Flag EF-1 alpha ALS Franz-Ulrich Hartl 232013 pEF1a FLAG-CHMP2B-d55-96 CHMP2B Flag ...sfGFP-CHMP2B-55-96 CHMP2B GFP CMV ALS Franz-Ulrich Hartl 232015 pCMV sfGFP-CHMP2B-3x-55-96 CHMP2B GFP CMV...pCMV CHMP2B-L4D F5D-d10-52-3xHA (deltaα1) CHMP2B HA CMV ALS Franz-Ulrich Hartl 232005 pCMV CHMP2B-L4D ...242393 pCMV CHMP2B-L4D,F5D-V82E-3XHA CHMP2B HA CMV ALS Franz-Ulrich Hartl 242416 pCMV CHMP2B-L4D,F5D-M85E...

  3. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... you'll find in this post: MagIC-Cryo-EM for enrichment and structure determination LEA proteins protect...marking astrocytes MagIC-Cryo-EM for sample enrichment and structure determination By Mike Lacy Conventional...and Concentration (MagIC)-cryo-EM that combines enrichment and imaging of proteins captured on magnetic ...proteins ending with a GFP nanobody (Figure 1). This enrichment streamlines the purification and minimizes sample...    Figure 1: MagIC-cryo-EM for sample enrichment and structure determination. A) Schematic of ...

  4. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...and easier to use with other strains of E. coli, Schmidl et al. condensed the system to two streamlined ...gene expression at very high or very low levels. Schmidl et al. chose to optimize these systems to make ...enable the assembly of larger systems. The work of Schmidl et al. also represents a large step forward in ...light-switchable Escherichia coli two-component systems. Schmidl SR, Sheth RU, Wu A, Tabor JJ. ACS Synth Biol. 2014...

  5. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    ...display to increase the enrichment of target-specific sybodies. Final enrichment via enzyme-linked immunosorbent...Ribosome display The Seeger lab starts with a “pre-enrichment” selection step using ribosome display because...sybody candidate. These tags are used in a final enrichment step for identifying sybodies that bind strongly...

  6. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... fluorescence. Transient reporter for editing enrichment (TREE) The Wang and Brafman lab developed a transient... transient reporter for editing enrichment (TREE) to purify single base edited cells without the need ...A fluorescent reporter for quantification and enrichment of DNA editing by APOBEC–Cas9 or cleavage by ...Brafman DA (2019) A transient reporter for editing enrichment (TREE) in human cells. Nucleic Acids Research...

  7. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    ...doi.org/10.1021/acssynbio.0c00581   New tool for enrichment of ABE-targeted cells By Ellie Adams Single base...mediated adenosine transient reporter for editing enrichment”, or XMAS-TREE, to identify base edited cell ...the past.   Figure 1: Identification and enrichment use of XMAS-TREE. a) Cells are transfected with...

  8. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...generated a strong streptavidin signal (~250-fold enrichment) when compared to a scrambled RNA motif control...labeling with BASU still generated a >30-fold enrichment compared to E. coli’s BirA*. Faster labeling ...-associated sequences had lower IREB2 binding enrichment compared to wild-type sequences and that loss...

  10. Bikard Lab - CRISPR Repression Collection

    Type
    Collection
    ...integration of the aTc-inducible pTet-dCas9 in the attachment site of the phage 186. Detailed information about...promoter integrated in the chromosome at phage attachment sites. The levels of the two reporters, mCherry...

  11. A Scientist's Guide to Ebola

    Type
    Blog Post
    ...virion surface – making it critical for viral attachment and fusion. Once inside a host cell, new evidence... of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading For those looking...

  12. CRISPR Guide

    Type
    Collection
    ...A., Waldhauer, M. C., Börner, K., Fakhiri, J., Schmelas, C., Dietz, L., Grimm, D., Correia, B. E., Eils...Simpson, D., Zhao, A., Li, H., Yan, W., Goudy, L., Schmidt, R., Solley, S. C., Gilbert, L. A., Chan, M. M.... 31189177 Strecker, J., Ladha, A., Gardner, Z., Schmid-Burgk, J. L., Makarova, K. S., Koonin, E. V., &...: 34478496 Yarnall, M. T. N., Ioannidi, E. I., Schmitt-Ulms, C., Krajeski, R. N., Lim, J., Villiger, L...

  16. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...., Allam, A., Kissling, L., Marquart, K. F., Schmidheini, L., Solari, C., Balázs, Z., Krauthammer, M.,... A., Tálas, A., Kissling, L., Benvenuto, E., Schmidheini, L., Schep, R., Damodharan, T., Balázs, Z., Janjuha...

  18. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    ...population is key. Loss of representation due to enrichment or depletion of specific gRNAs can lead to skewed...several cell types can strengthen your results. Enrichment or depletion of multiple gRNAs targeting the ...

  19. An Introduction to Adenovirus

    Type
    Blog Post
    ...biology of adenoviruses (AdVs). AdVs are ubiquitous (Schmitz et al., 1983), yet rarely cause severe disease ...doi.org/10.3181/00379727-84-20714. PMID: 13134217. Schmitz, H., Wigand, R., & Heinrich, W. (1983). Worldwide...

  20. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...is used to separate the beads from the media (Brechmann et al. al., 2021) while the media is aspirated....2019.12.017. Epub 2019 Dec 28. PMID: 31891733. Brechmann NA, Schwarz H, Eriksson PO, Eriksson K, Shokri...
Showing: 1 - 20 of 109 results