...blue (SF-Azurite-iGluSnFR), green (SF-iGluSnFR), and yellow (SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate...updates to iGluSnFR and SF-iGluSnFR have made it clear that it’s time to update our iGluSnFR post! Here...mice. Second Generation iGluSnFR: SF-iGluSnFR In the second generation of iGluSnFR, the Looger lab swapped... original 2018 blog post. The origins of iGluSnFR   iGluSnFR was developed in 2013 by Loren Looger’s lab... generation of the SF-Venus-iGluSnFR (itself an improvement over iGluSnFR), Kaspar Podgorski’s lab have...is available here. Upgrades to iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following...of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP between two halves ...

...reader up-to-date on the latest version of the iGluSnFR glutamate sensorRUBY-Red Siliques: Guest blogger...sharing the data on the mAb3D atlas website.  The iGluSnFR3 Glutamate Sensor: Guest blogger Abhi Aggarwal ...

...GENIE Glutamate Sensor: iGluSnFr 98929 pAAV.hSyn.iGluSnFr.WPRE.SV40 Syn iGluSnFr none Constitutive 1, 5...Sensors iGABASnFR iGABASnFR2 Glutamate Sensors iGluSnFr SF-iGluSnFr Glucose Sensors iGlucoSnFr Norepinephrine...Sensor: SF-iGluSnFr 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn SF-iGluSnFR.A184S none Constitutive 1, 9... 5, 9 Looger 98930 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 GFAP iGluSnFr none Constitutive 1, 5, 9 Looger 98931...98931 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Syn iGluSnFr none Cre dependent 1, 5, 9 Looger 98932 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40....Flex.iGluSnFr.WPRE.SV40 CAG iGluSnFr none Cre dependent 1, 5, 9 Looger 100889 pAAV.GfaABC1D.GluSnFr.SV40...Constitutive 5 Khakh 175180 pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt Syn iGluSnFr3 v857 none Cre dependent...

... New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR from Loren Looger’s lab,...compared with earlier SF-iGluSnFR. Image from Aggarwal 2022.   Find iGluSnFR3 plasmids here! Find viral...Kaspar Podgorski and colleagues have now released iGluSnFR3 (Aggarwal et al. 2022). This new version has higher...from the authors in this blog post. Figure 2: iGluSnFR3 displays enhanced brightness, expression, and ...viral prep here: pAAV.hSyn.FLEX.iGluSnFR3.v857.PDGFR.codonopt    Aggarwal A, et al. Glutamate indicators...In addition to the viral preps noted above for iGluSnFR3 and JEDI-2P, several biosensors already in our...

...flame extracellular glutamate sensor pCMV(MinDis).iGluSnFR. Picking your reporter Don’t forget about the ...

...AAVrg pAAV-hSyn-GRAB_NE1m Glutamate sensors iGluSnFR is a useful tool from Loren Looger’s lab at Janelia...neuroscientists to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate...Serotype Name 106174  AAVrg pAAV.hSynapsin.SF-iGluSnFR.A184S Viral vectors coming soon! These vectors...

... similar to the protein which gave rise to the iGluSnFR sensor. However, there are no known bacterial ...

... (H2O2 sensor), ArcLight (voltage sensor), and iGluSnFr (glutamate sensor). More examples of these biosensors...

...-based Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and...axonal boutons of neurons expressing iGluSnFR3, iGluSnFR4f, and iGluSnFR4s. C) Spike-triggered averages for... library of iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation... Kaspar Podgorski Labs now has a new version: iGluSnFR4, developed by the GENIE Project team at HHMI's...deactivation) and iGluSnFR4s (slow deactivation). Both have significantly improved brightness, kinetics, ..., and sensitivity. iGluSnFR4f is well-suited for imaging rapid synaptic events such as in the somatosensory...somatosensory cortex, while iGluSnFR4s produces larger signal-to-noise ratios in two-photon imaging, making ...

...Frommer Glutamate Ultrafast glutamate sensors (iGluSnFR variants) Ultrafast glutamate sensors resolve ...2023 Jan 9;12:e84029. Andrew Plested Glutamate iGluSnFR glutamate sensor with improved trafficking to ...Glutamate Fourth generation glutamate sensors (iGluSnFR4) for improved sensitivity and scale (4s) or speed...Glutamate Third generation glutamate sensors (iGluSnFR3) for improved imaging of synaptic transmission...displayed variants of fluorescent glutatmate sensors (iGluSnFR1) Genetically Encoded Glutamate Indicators with...

...reporter (iGluSnFR3) to study glutamate, a signaling molecule in the brain. “We hope that iGluSnFR3 will enable...and Tim Hanson, are characterizing variants of iGluSnFR3 in vivo and have used it to detect glutamate release...these biosensors.   Figure 1: Schematic of iGluSnFR3 (left). GluSnFR3 in pMinDisplay (PDGFR anchoring...

..., Tobias Rose AV-1-PV2723 98929-AAV1 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2724 98931... 98931-AAV1 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV2725 98932-AAV1 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40...Douglas Kim AV-1-PV2914 98930-AAV1 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-1-PV3080... Baljit Khakh AV-5-PV2723 98929-AAV5 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV2724 98931... 98931-AAV5 pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV2725 98932-AAV5 pAAV.CAG.Flex.iGluSnFr.WPRE.SV40...Douglas Kim AV-5-PV2914 98930-AAV5 pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-5-PV3107...Eric Schreiter AV-9-PV2723 98929-AAV9 pAAV.hSyn.iGluSnFr.WPRE.SV40 Biosensor Loren Looger AV-9-PV2724 98931...

...Biosensor Tian New viral vector pAAV.hSynapsin.SF-iGluSnFR.A184S AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3...

...Serotype Name 106176  AAV1  pAAV.hSynapsin.SF-iGluSnFR.S72A New calcium sensors Plasmid Serotype Name...

...Looger Lab now available in AAV1. Get GFAP.SF-iGluSnFR.A184S-AAV1 here New Dual and Triple feature INTRSECT...

...Calcium sensor Looger 106174 pAAV.hSynapsin.SF-iGluSnFR.A184S Syn Tight affinity glutamate sensor Glutamate...