We narrowed to 16 results for: mOrange
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TypeBlog Post...mTFP1 can be used as a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters...
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Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools
TypeBlog Post...and QuasAr, as well as the eFRET sensors, QuasAr-mOrange and MacQ-mCitrine. All of these sensors can detect... -
Fluorescent Protein Guide: Empty Backbones
TypeCollection... Expression mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial...Worm/Sea urchin mOrange-N1 - Mammalian Expression mOrange-C1 - Mammalian Expression mOrange-pBAD - Bacterial...Expression mOrange2 549 565 35 6.5 4.5 h Monomer mOrange2-N1 - Mammalian Expression mOrange2-C1 - Mammalian...Expression LSSmOrange 437 572 23 5.7 2.3 h Monomer pLSSmOrange-C1 - Mammalian Expression pLSSmOrange-N1 - Mammalian...Bacterial Expression pCS2+8CmOrange - Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmOrange - Zebrafish/Xenopus/Worm...Mammalian Expression PSmOrange 548 634 565 662 480 58 9 6.2 5.6 1.6 h Monomer pPSmOrange-N1 - Mammalian Expression...Expression pPSmOrange-C1 - Mammalian Expression pFA6a-link-yoPSmOrange-SpHis5 - Yeast Expression PSmOrange2... -
Tips for Using FRET in Your Experiments
TypeBlog Post...interest fused to the N-terminus of LSSmOrange pLSSmOrange-C1 Orange Mammalian Express a gene of interest... (a GFP variant) commonly used with mRuby2 pLSSmOrange-N1 Orange Mammalian Express a gene of interest...interest fused to the C-terminus of LSSmOrange mRuby2 Red Mammalian Expresses mRuby2 (a RFP variant) commonly... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post...Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the default state. In... both fluorescence and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...proteins Newly developed photoconvertible FPs like PsMOrange and mEos add another layer of complexity to imaging...photoswitchable orange-to-far-red fluorescent protein, PSmOrange. Nat Methods. 2011 Jul 31;8(9):771-7. doi: 10.1038... -
28 Hot Plasmid Technologies from 2015
TypeBlog Post...developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein which transitions... sequence enhances mitochondrial targeting of PSmOrange, as well as other photoactivatable proteins like...like PAGFP. Co-expression of LOC8R-PSmOrange and LOC8R-PAGFP provides a novel strategy for assaying the...subpopulations within the same cell; by photoswitching PSmOrange and photoactivating PAGFP in separate subpopulations... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog Post... orange, and red fluorophores. The spectra for mOrange2 (scaffold of OCaMP) are shaded in orange. Data...et al., 2025), based on the fluorescent protein mOrange2 (Figure 2). Figure 2: OCaMP structure ... -
Fluorescent Protein Guide: Biosensors
TypeCollection... (Arch(D95N), MacQ-mCitrine, QuasAr and QuasAr-mOrange) Rhodopsin-based voltage imaging tools for use ... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog Post... by integrating a SL-insensitive fluorophore (LSSmOrange) as an internal control and a SL-sensitive fluorophore... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog Post...red-shifted FRET sensor for zinc using variants of mOrange2 and mCherry as donor and acceptor domains. He ... -
Which Fluorescent Protein Should I Use?
TypeBlog Post... green or red light. For example, T-Sapphire, LSSmOrange, and LSSmKate. Fluorescent Sensors: These FPs... -
Fluorescent Proteins: FRET
TypeCollection...pBAD , pLSSmOrange-mKate2 LSSmOrange* mScarlet3 437 0.45 592 104,000 0.75 5.8 4.3 pLSSmOrange-N1 , pmScarlet3...2.9 mVenus N1 , mKO2-N1 mOrange2 mCherry 549 0.60 610 72,000 0.22 6.1 4.4 mOrange2-N1 , mCherry2-N1 miRFP670...-N1 , pmiRFP720-N1 LSSmOrange* mKate2 437 0.45 633 62,500 0.40 5.6 3.7 pLSSmOrange-N1 , mKate1.31-pBAD... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...EGFP Carol Otey 31923 pPaxillin-PSmOrange Focal Adhesions paxillin PSmOrange Vladislav Verkhusha 79999 pZyxin-miRFP703... -
Luciferase Plasmid Collection
TypeCollection...acceptor. LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging...