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We narrowed to 37 results for: mScarlet

Showing: 1 - 20 of 37 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ... The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...structural studies of E3 ligase cereblon Optimized mScarlet-based tags for live imaging in C. elegans CRISPR...s41467-024-52871-9. PMID 39406745. Optimizing mScarlet tags for live imaging in C. elegans By Alyssa ...

  2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type
    Blog Post
    ...protein (mScarlet) with either a seven or eight amino acid linker between them. The Ace-mScarlet voltage...results in higher quenching of mScarlet emission. Figure 2: Ace-mScarlet is a fusion between the voltage-sensitive...inhibitory rhodopsin Ace2N and mScarlet. Ace2N quenches a proportion of mScarlet's emission via FRET. Image ...created two plasmids containing a red-shifted Ace-mScarlet, a fusion of a voltage-sensitive inhibitory rhodopsin...blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et...

  3. New Viral Vectors - Fall 2024

    Type
    Blog Post
    ...pAAV-Ef1a-DIO mScarlet AAV5 Controls Karl Deisseroth New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV5 Optogenetics...Ofer Yizhar New viral prep pAAV_hSyn-DIO-PdCO-mScarlet-WPRE AAV1, AAV5 Optogenetics Ofer Yizhar New...Edward Boyden New serotype pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE AAV1 Optogenetics Karl Deisseroth New serotype...

  4. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (...compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H with M164H...compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants...New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed ...synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate...protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat...Methods. 2017. PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl...

  5. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    ...Listen to Our Segment on mCyRFP1 Hot Plasmid 4: mScarlet - a new, bright, and monmeric red fluorescent ...fluorescent protein.  Listen to Our Segment on mScarlet Special thanks to our sound engineer/producer, Eddy Page...

  6. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...service with multiple serotypes pAAV_EF1a-DIO-PdCO-mScarlet-ER-miniWPRE AAV1, AAV5 Optogenetics Yizhar New...with multiple serotypes pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAV1, AAV5 Optogenetics Yizhar New viral ...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...: Ace-mScarlet is a fusion between the voltage-sensitive inhibitory rhodopsin Ace2N and mScarlet. Ace2N...Ace2N quenches a proportion of mScarlet's emission via FRET.  Image from Beck et al, 2019 under CC BY 4.0...

  11. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved...

  12. New Viral Vectors - Spring 2025

    Type
    Blog Post
    ...Jordane Dimidschstein New serotype pAAV_hSyn-PdCO-mScarlet-WPRE AAV1 Optogenetics Ofer Yizhar New viral ...

  13. Optogenetics AAV Preps

    Type
    Collection
    ...124650 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 CaMKII C1V1 (t/t) mScarlet Constitutive 9 Christopher Harvey...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 CaMKII ChrimsonR (soma-targeted) mScarlet Constitutive 1, 9 Christopher...pAAV-CaMKIIa-ChRmine-mScarlet-Kv2.1-WPRE CamKII ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive...-hSyn-ChRmine-mScarlet-Kv2.1-WPRE Syn ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8...pAAV-Ef1a-DIO-ChRmine-mScarlet-WPRE EF1a ChRmine (high-photocurrent, red-shifted) mScarlet Cre dependent 1,...137158 pAAV-nEF-ChRmine-mScarlet nEF ChRmine (high-photocurrent, red-shifted) mScarlet Constitutive 8 Karl...Deisseroth 125712 pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE CaMKII eOPN3 mScarlet Constitutive 1, 5, rg* Ofer Yizhar...

  16. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen... 98818 4xmts-mScarlet-I Mitochondria 4x Mitochondrial targeting sequence (COX8) mScarlet-I Dorus Gadella...Gadella 98821 Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella ...mTurquoise2 Dorus Gadella 98827 Lamp1-mScarlet-I Lysosomes Lamp1 mScarlet-I Dorus Gadella 55073 mCherry-Lysosomes...85054 pLifeAct_mScarlet_N1 Actin filaments LifeAct mScarlet Dorus Gadella 85056 pLifeAct_mScarlet-i_N1 Actin...mTurquoise2 Dorus Gadella 85053 pmScarlet-i_H2A_C1 Nucleus H2A mScarlet-I Dorus Gadella 18982 pHIV-H2BmRFP...Gadella 85063 pmScarlet_peroxisome_C1 Peroxisomes Peroximal targeting signal (SRL) mScarlet Dorus Gadella...

  17. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...fluorescent proteins, PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP had...Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness...

  18. Control AAV Preps

    Type
    Collection
    ...pAAV-CaMKIIa-mScarlet CamKIIa mScarlet Constitutive 8 Karl Deisseroth 131001 pAAV-hSyn-mScarlet Syn mScarlet...rg* Brandon Harvey 131002 pAAV-Ef1a-DIO mScarlet EF1a mScarlet Cre dependent 1, 5 Karl Deisseroth 50457...

  19. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...Deisseroth New viral prep pAAV-CaMKIIa(0.4)-eOPN3-mScarlet-WPRE AAVrg Optogenetics Ofer Yizhar New serotype...
Showing: 1 - 20 of 37 results