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We narrowed to 11 results for: mScarlet-H

Showing: 1 - 11 of 11 results
  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and mScarlet-I3 constructs. Scale bar...hours. In order to use the latest mScarlet tags, mScarlet3 and mScarlet-I3, Oliver Hobert’s lab recently...like mScarlet are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow...4 protein, mScarlet3 was 50% brighter than the original worm-optimized wrmScarlet. mScarlet-I3, while ... The team designed coding sequences for mScarlet3 and mScarlet-I3 that were codon-optimized for C. elegans...structural studies of E3 ligase cereblon Optimized mScarlet-based tags for live imaging in C. elegans CRISPR...s41467-024-52871-9. PMID 39406745. Optimizing mScarlet tags for live imaging in C. elegans By Alyssa ...
  2. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...compared to mScarlet, fluorescent lifetime of 3.1 ns, quantum yield of 0.54) and mScarlet-H with M164H ...characterized: mScarlet (fluorescent lifetime of 3.9 ns, quantum yield of 0.70), mScarlet-I with T74I (...compared to mScarlet, fluorescent lifetime of 1.3 ns, quantum yield of 0.20). All three mScarlet variants...New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently developed ...synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a combination of deliberate...protein fusions. The Gadella lab has deposited all mScarlet constructs with Addgene. Bindels DS, et al. Nat...Methods. 2017. PubMed PMID: 27869816 Listen to our mScarlet Podcast Segment    Photocleavable protein PhoCl...
  3. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mTurquoise2 Dorus Gadella 98816 3xnls-mScarlet-I Nucleus 3xNLS mScarlet-I Dorus Gadella 98876 4xmts-mNeonGreen... 98818 4xmts-mScarlet-I Mitochondria 4x Mitochondrial targeting sequence (COX8) mScarlet-I Dorus Gadella...Gadella 98821 Lck-mScarlet-I Plasma membrane Lck N-terminal lipidation signal mScarlet-I Dorus Gadella ...mTurquoise2 Dorus Gadella 98827 Lamp1-mScarlet-I Lysosomes Lamp1 mScarlet-I Dorus Gadella 55073 mCherry-Lysosomes... pTag-RFP-C-h-Rab5a-c-Myc Endosomes (early) Rab5a TagRFP James Johnson 79801 pTag-BFP-C-h-Rab5a-c-Myc ...pTag-RFP-C-h-Rab11a-c-Myc Endosomes (recycling) Rab11a TagRFP James Johnson 79805 pTag-BFP-C-h-Rab11a-c-...pTag-RFP-C-h-Rab4a-c-Myc Endosomes (recycling) Rab4a TagRFP James Johnson 79799 pTag-BFP-C-h-Rab4a-c-Myc...
  4. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    ...doi.org/10.1186/s13036-018-0100-0 Murakoshi, H., Horiuchi, H., Kosugi, T., Onda, M., Sato, A., Koga, N.,...ShadowR is used as a FRET acceptor to mRuby2 or mScarlet. Image: Murakoshi et al., 2019. ShadowR is ... a FRET acceptor in combination with mRuby2 or mScarlet as the donor in HeLa cells.   Find ShadowR for...doi.org/10.1007/bf00428249 Chan MCY, Karasawa S, Mizuno H, Bosanac I, Ho D, Privé GG, Miyawaki A, Ikura M (2006...
  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Expression mScarlet 569 594 71 5.3 2.9 h Monomer pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning...Maturation Structure Plasmids mScarlet-H 551 592 15 4.8 4.4 h Monomer pmScarlet-H_C1 - Mammalian Expression...574 62 4.4 1.5 h Dimer TurboRFP-pBAD - Bacterial Expression tdTomato 554 581 95 4.7 1 h Tandem-dimer pCSCMV...mApple-pBAD - Bacterial Expression mScarlet-I3 568 592 68 4.2 2 min Monomer pmScarlet-I3_C1 - Mammalian Expression...Vector - Mammalian Expression mScarlet-I 569 593 57 5.4 36 min Monomer pmScarlet-i_C1 - Mammalian Expression... Plasmids mKusabira-Orange (mKO) 548 559 31 5 4.5 h Monomer pQC mKorange IX - Mammalian Expression mKusabira...- Bacterial Expression mOrange 548 562 49 6.5 2.5 h Monomer pET mOrange LIC cloning vector (u-mOrange)...
  6. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...proteins that had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random...semi-random mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved...384–391. https://doi.org/10.1038/nchem.2447.  Lee, H., Yang, N., & Cohen, A. E. (2011). Mapping Nanomagnetic...
  7. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...fluorescent proteins, PinkyCaMP is the first based on mScarlet. Compared to RCaMP3 and jRGECO1a, PinkyCaMP had...Fink, R., Imai, S., et al. (2024). PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness...microenvironments here! Mensah, M. A., Niskanen, H., et al. (2023). Aberrant phase separation and nucleolar... it out! Find AAV-CRISPRa plasmids here! Nguyen, H. P., et al. (2025). Implantation of engineered adipocytes...
  8. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ...Libraries are available with T-Sapphire, EGFP, or mScarlet reporters, so you can choose a color compatible... Find ATLAS plasmids here! Rivera, J. F., Huang, H., et al. (2025). ATLAS: a rationally designed anterograde...
  9. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Berndt A, Grosenick L, Zalocusky KA, Bernstein H, Swanson H, Perry C, Diester I, Boyce FM, Bass CE, Neve...Fon-bREACHes-EYFP Flp AND NOT Cre No 137158 pAAV-nEF-ChRmine-mScarlet None Yes 137159 pAAV-nEF-Con/Fon-ChRmine-oScarlet... in a new window) Yu K, Ahrens S, Zhang X, Schiff H, Ramakrishnan C, Fenno L, Deisseroth K, Zhao F, Luo...
  10. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...3145. Hadley Sikes Hydrogen Peroxide (H 2 O 2 ) Monitoring of basal H 2 O 2 levels with peroxiredoxin-based...Belousov Hydrogen Peroxide (H 2 O 2 ) Cytosolic or mitochondrial sensor for H 2 O 2 oxidation (roGFP2-Orp1...Calcium Red calcium sensor PinkyCaMP PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness... voltage sensor to detect neural activity (Ace-mScarlet) A high-speed, bright, red fluorescent voltage...2012;7(6):e38591. Sakiko Okumoto Hydrogen Peroxide (H 2 O 2 ) Fluorescent reporter of cytosolic hydrogen...peroxiredoxin-based probes Real-time monitoring of basal H 2 O 2 levels with peroxiredoxin-based probes. Nat Chem Biol... Jun;12(6):437-43. Tobias Dick Hydrogen Peroxide (H 2 O 2 ) HyPer biosensor to measure changes in intracellular...
Showing: 1 - 11 of 11 results