We narrowed to 12 results for: nano bret

... Enhanced Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase...moieties in their Nano-Lanterns with NLuc and called their improved creations Enhanced Nano-Lanterns. These...the furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals...FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase to excite the Venus fluorescent.... 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc BRET reporters enable sensitive in vivo optical imaging...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nature Comm. 7, 13268...

... at Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase...different contexts. Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to...enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous Nano-lanterns...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear...separates the signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used...explained further in Takai et al. Nano-lanterns and ready-made Nano-lantern biosensors are available from...laboratory has assembled a collection of multicolored nano-lanterns for use in various applications, including...

...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins...analyze cell signaling pathways or miRNA knockdown. BRET for the best of both (fluorescent and luminescent...light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred...approach can test protein-protein interactions using BRET. A luciferase and a fluorescent protein are each...fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein emits light of a different... approach has even been used in biosensors where BRET reports on the change in conformation of a single...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...

...luciferase Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai...about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live...Nano-lanterns ) or NanoLuc® ( Enhanced Nano-lanterns and Antares ) for in vivo imaging. NanoBRET : Constructs using...LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other... using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors...plants. Bioluminescence Resonance Energy Transfer (BRET) Similar to FRET, energy transfer from the luciferase...

...variants of their Nano-lantern technology, first developed in 2012. The new cyan and orange Nano-lantern fusions...imaging. The Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...two improved Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by ...light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With...

...formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

...acceptor for Bioluminescence Resonance Energy Transfer (BRET) . Browse Addgene's Luciferase Plasmid Collection...Promega Plasmid Collection to find related tools for BRET assays. References Bajar, B. T., Wang, E. S., Zhang...distance between the fluorophores at the single-nanometer scale, scientists can use FRET to report on protein...

...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega...cells, and cell-free systems. NanoBiT Assays NanoLuc Binary Technology (NanoBiT) (Link opens in a new window...high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link opens in a new window) is a 19.1 ...thus preserving physiological relevance. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion protein expressed...analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting...

...Temperature Imaging with a Genetically Encoded Nanothermometer. Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...top Other Reporters ONE-GO Biosensors Kit includes BRET-based biosensors of G proteins activated by G-protein-coupled...Verkhusha Calcium Small near-infrared indicator iGECInano Design and Initial Characterization of a Small...):e35373. Susan Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering...Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations...Visualize intracellular tryptophan levels and flux Nanosensor detection of an immunoregulatory tryptophan influx...

...Woolard, J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics...HiBiT is a minimal 11-amino-acid fragment of the NanoBiT® complex, which, upon binding to its complementary... addition and a simple add-mix-measure method. NanoBiT® luciferase activity is compatible with both intra...LgBiT, and the authors used a p53 response-element NanoLuc reporter to show that the mutants abolished transcriptional...facilitating metastatic spread. The authors used NanoBiT assays to quantify ADAR1-HiBiT in cell lysates,...subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled...

...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...

...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation... Arrowsmith 211083 pNLF1-C_EIF2B2:1-351 EIF2B2 NanoLuc CMV VWM, CACH Cheryl Arrowsmith 211112 pBiT3.1-...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...