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We narrowed to 10 results for: nano bret

Showing: 1 - 10 of 10 results

  1. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ... Enhanced Nano-Lanterns to other cellular proteins. BRET-based biosensors utilizing NanoLuc® luciferase...moieties in their Nano-Lanterns with NLuc and called their improved creations Enhanced Nano-Lanterns. These...the furimazine substrate for NanoLuc luciferase. Both GpNLuc and OgNLuc BRET reporters produced bright signals...FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase to excite the Venus fluorescent.... 3. Schaub, F.X., et al. (2015) Fluorophore-NanoLuc BRET reporters enable sensitive in vivo optical imaging...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...Coupling optogenetic stimulation with NanoLuc-based luminescence (BRET) Ca++ sensing. Nature Comm. 7, 13268...

  2. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ... at Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase...different contexts. Improving Nano-lanterns In 2015, Takai et al. expanded the Nano-lantern color palette to...enhanced Nano-lanterns (eNL) described in Suzuki et al. improve the brightness of previous Nano-lanterns...appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires a linear...separates the signals of various Nano-lanterns, but it does not isolate each Nano-lantern, so Takai et al. used...explained further in Takai et al. Nano-lanterns and ready-made Nano-lantern biosensors are available from...laboratory has assembled a collection of multicolored nano-lanterns for use in various applications, including...

  3. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...blog Luminescent Imaging with Nano-lanterns  Technologies Enabled by NanoLuc® Luciferase Fluorescent Proteins...analyze cell signaling pathways or miRNA knockdown. BRET for the best of both (fluorescent and luminescent...light. Bioluminescence Resonance Energy Transfer (BRET) works the same way, except that energy is transferred...approach can test protein-protein interactions using BRET. A luciferase and a fluorescent protein are each...fluorescent protein close enough, energy is transferred by BRET and the fluorescent protein emits light of a different... approach has even been used in biosensors where BRET reports on the change in conformation of a single...Plasmid Collection, including tools using NanoLuc®, NanoBiT, and more. Which luciferase and assay have...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...luciferase Liqun Luo 51970 Nano-lantern/pcDNA3 Nano-lantern CMV Mammalian expression of Nano-lantern Takeharu Nagai...about BRET on our blog . Nano-Lanterns: Fluorescent proteins fused to Renilla luciferase ( Nano-lanterns...Kit , and G-CASE : BRET-based sensors for reporting on GPCR activity. AIMTOR : BRET biosensor for live...Nano-lanterns ) or NanoLuc® ( Enhanced Nano-lanterns and Antares ) for in vivo imaging. NanoBRET : Constructs using...LumiFluors: EGFP-NanoLuc ( GpNLuc ) and LSSmOrange-NanoLuc ( OgNLuc ) fusions for BRET-based imaging and other...Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent Imaging with Nano-lanterns Promega Plasmid... using NanoLuc® as a BRET energy donor and HaloTag labeled with a fluorophore as the acceptor. LumiFluors...

  5. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...variants of their Nano-lantern technology, first developed in 2012. The new cyan and orange Nano-lantern fusions...imaging. The Nano-lanterns rely on the principle of bioluminescence resonance energy transfer (BRET), whereby...that use Bioluminescence Resonance Energy Transfer (BRET) were developed by the Johnson Lab . Characteristics...cytoplasmic retention insensitivity to other ions In BRET, A mutant Renilla luciferase, catalyzes the oxidation...luminescent siganls and a ratio is calculated (the BRET ratio), which allows for compensation of varying...two improved Light Inducible Dimers (iLID), iLID nano and iLID micro, which differ from each other by ...light/dark affinity range (130nM to 4.7µM for iLID nano and 800nM to 47µM for iLID micro; Figure 1). With...

  6. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...formation of Gα-GTP by BRET. The biosensor consists of a YFP-tagged G protein and a NanoLuc® (Nluc) Luciferase-fused...Garcia-Marcos’ lab recently developed a collection of BRET-based ONE vector G-protein Optical (ONE-GO) biosensors...

  7. Promega Plasmid Collection

    Type
    Collection
    ...proximity in living cells. NanoBRET PPI assays use NanoLuc Luciferase as a BRET energy donor and HaloTag...window) and NanoBRET assays (Link opens in a new window) NanoLuc®, HaloTag®, NanoBiT®, and NanoBRET® are Promega... physiological relevance. NanoBiT Assays NanoLuc Binary Technology (NanoBiT) (Link opens in a new window...high-throughput screening in drug discovery. NanoLuc Fusions NanoLuc (Link opens in a new window) is a 19.1 ...interaction, association, and dissociation. NanoBRET Assays NanoBRET (Link opens in a new window) is an energy...competitive displacement of a NanoBRET tracer, reversibly bound to a NanoLuc luciferase-fusion protein expressed...analysis and fusion vectors containing tags such as NanoLuc, HaloTag, SmBiT, and LgBiT, useful for detecting...

  8. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Temperature Imaging with a Genetically Encoded Nanothermometer. Nano Lett. 2022 Jul 27;22(14):5698-5707. Takeharu...13. pii: tpc.20.00241. Markus Schwarzländer NADPH BRET semi-synthetic biosensor measuring the NADPH concentration...):e35373. Susan Amara , Susan Ingram Citrate Nanosensors for in vivo measurements of citrate (CitA) Engineering...Engineering genetically encoded nanosensors for real-time in vivo measurements of citrate concentrations...Visualize intracellular tryptophan levels and flux Nanosensor detection of an immunoregulatory tryptophan influx...

  9. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    ...adenovirus, and inorganic delivery tools such as nanoparticles. These technologies can have serious adverse...untreatable until 2008. Following a single dose of subretinal administration of rAAV2 carrying the human gene...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Corporation 237172 EIF2AK2(252-551)-NanoLuc Fusion Vector EIF2AK2 NanoLuc CMV VWM disease Promega Corporation... Arrowsmith 211083 pNLF1-C_EIF2B2:1-351 EIF2B2 NanoLuc CMV VWM, CACH Cheryl Arrowsmith 211112 pBiT3.1-...Vector VCP Halo CMV ALS Promega Corporation 237148 NanoBRET Assay Vector, TREM2-HaloTag TREM2 Halo CMV Alzheimer's...
Showing: 1 - 10 of 10 results