Exploring Applications of the Bioluminescent HiBiT Tag
Type
Blog Post
... L. P., & Wood, K. V. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel ....67.1.509
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Promega Plasmid Collection...and structurally complex. The tumor suppressor TP53 (p53) is one example of these difficult-to-study genes... GC. HiBiT-TP53 expression was detected by blotting with LgBiT, and the authors used a p53 response-element...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the presence...partner LgBiT, reconstitutes a fully functional luciferase enzyme (Figure 1). Addition of substrate generates...and a simple add-mix-measure method.
NanoBiT® luciferase activity is compatible with both intra- and extracellular...