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We narrowed to 4 results for: p53 luciferase p53 luc reporter

Showing: 1 - 4 of 4 results

  1. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    ... L. P., & Wood, K. V. (2012). Engineered luciferase reporter from a deep sea shrimp utilizing a novel ....67.1.509  More resources from Addgene Luciferase 101 Luciferase Plasmid Collection Promega Plasmid Collection...and structurally complex. The tumor suppressor TP53 (p53) is one example of these difficult-to-study genes... GC. HiBiT-TP53 expression was detected by blotting with LgBiT, and the authors used a p53 response-element...response-element NanoLuc reporter to show that the mutants abolished transcriptional activity, even in the presence...partner LgBiT, reconstitutes a fully functional luciferase enzyme (Figure 1). Addition of substrate generates...and a simple add-mix-measure method. NanoBiT® luciferase activity is compatible with both intra- and extracellular...

  2. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    ...fragment Constant rate of p53 tetramerization in response to DNA damage controls the p53 response. Gaglia G,...Fluorescent Biosensors Collection Split Luciferase systems, including NanoLuc®, DULIP, LuTHy Other split protein... (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging of Protein-Protein Interactions...green-yellow or orange-red fluorogen A split fluorescent reporter with rapid and reversible complementation. Tebo...design. We hope you find these plasmids useful. Good luck, and happy experimenting! References and Resources...

  3. Luciferase Plasmid Collection

    Type
    Collection
    ...CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...Multiple Multiple Multi-luciferase reporter vector including five transcriptional reporters for NF-kb, TGF-b,...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often...CBRluc and CBGluc , respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase...Firefly luciferase. Renilla luciferase is co-expressed. Oskar Laur 87070 pcDNA3.1-Nanoluc-ccdB NanoLuc® Creation...process. Lynne Postovit 64034 pGL4Luc-RLuc Firefly, Renilla Dual reporter reporter vector allowing the study...

  4. Validated gRNA Sequences

    Type
    Collection
    ...GATCCACAAGTTACAATTGG 46170 cut S. pyogenes 23817069 Calarco Kras, p53, and Lkb1 M. musculus multiple, see article 60224...pyogenes 23929339 Sheen pGL3-Basic-8x-gRNA-eGFP reporter synthetic AAAGGTCGAGAAACTGCAAA 60719 activate ...AGACGACCCTGTCATCCGCA 74188 cut S. pyogenes 26627737 Moffat Luciferase ACAACTTTACCGACCGCGCC 74190 cut S. pyogenes 26627737...25619936 Sato NanoLuc synthetic AGCTTACGCCACCCTGTTCC 68897 interfere S. pyogenes 26918244 Lu NanoLuc synthetic...26918244 Lu NanoLuc synthetic TCACGCTCACACCCAGGTTC 68895 interfere S. pyogenes 26918244 Lu NanoLuc synthetic...GTGAACCGCATCGAGCTGAA 41819 cut S. pyogenes 23287722 Church GLuc synthetic GATCTAGATACGACTCACTAT 68422 CRISPR-display...GTCAATTGTTCTCTTTCTAT 64331 cut S. pyogenes 25281382 Jin Trp53 M. musculus CCTCGAGCTCCCTCTGAGCC 59910 cut S. pyogenes...
Showing: 1 - 4 of 4 results