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We narrowed to 71 results for: lentivirus vector

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  1. Viral Vectors 101: Viral Vector Elements

    Type
    Blog Post
    Published
    July 25, 2023, 1 p.m.
    ...of viral vectors here! Need Virus? Check out Addgene's Viral Service! Types of viral vectors Of the many...gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The components of these vectors are also...’ll breakdown the vector components first by virus and then by function. Lentivirus/gamma-retrovirus Lentiviruses... Lentiviruses are a class of retrovirus (so all lentiviruses are retroviruses, but not all retroviruses...retroviruses are lentiviruses.) This means that lentivirus and gamma-retrovirus share a lot of core components,...types, but most vectors are based on adenovirus 5 (Ad5).     Fig. 3 - Viral vector components of...of adenovirus Building viral vectors Addgene has many viral vectors and helper plasmids for all the...

  2. Top Requested Lentivirus and AAV of 2016

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    Blog Post
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    Jan. 6, 2017, 3:56 p.m.
    ...requested lentivirus and AAV of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9... is a dual-vector system, meaning it is typically used in conjunction with a second vector that delivers... gRNA lentiviruses). Separating Cas9 and the gRNA into two constructs (i.e., using a dual-vector system... Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that...lentiCas9-blast lentivirus from Addgene, you could use it in conjunction with gRNA lentiviruses easily made...Viral Service and began delivering ready-to-use lentivirus and adeno-associated virus (AAV) to scientists... 2016, we expanded our viral inventory to 25 lentiviruses and 25 AAVs. These viruses have been distributed...

  3. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ... is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G ... producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other...promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help determine...widely used and requested because it enables lentiviruses to infect many different types of cells. This...check out our lentivirus guide page. For more protocols and information, be sure to check out the Trono Lab...

  4. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest, and the...Q1: Are retroviruses and lentiviruses the same thing? Can I package lentivirus with retroviral packaging...3rd-generation transfer vectors apart? A3: Addgene defines 2nd- or 3rd-generation transfer vectors based on whether...between 2nd- and 3rd-generation vectors? What does this mean for transfer vectors? A4: The lentiviral packaging...largely based upon the vector system used and the transgene insert encoded by the vector." The table below ...Lentiviruses are useful and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses...gamma-retroviruses that can only infect dividing cells, lentiviruses can infect dividing and non-dividing cells.  ...

  5. Getting the Most from Your Lentiviral Transduction

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    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...for using lentivirus, infect and select, seems simple, in actuality, many find using lentivirus to be time...targets for a lentivirus are determined by the envelop protein. Many times lentiviral vectors are engineered...Blog Get Tips for Titering Your Lentivirus Learn All About Viral Vector Elements Learn How to Do Genome-Wide... CRISPR Lentiviral Vectors Additional Resources on Addgene.org Find Viral Vectors for Your Research ...Things Lentivirus with our Lentiviral Guide Pages Deposit Your Lentiviral Vectors ...Lentiviruses are a powerful laboratory tool often employed to establish cell lines that stably express...infection, happy virus is the second. When harvesting lentivirus, some labs prefer to collect and pool the harvests...

  6. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    Published
    March 21, 2017, 2:30 p.m.
    ...property makes the lentivirus an attractive choice for a gene transfer vector. Lentiviral vectors Although retroviruses...your experiment using the lentiviral vector system. Viral vectors have been increasingly popular in fundamental...primary cells. Amongst the different vectors used, lentiviral vector constructs have proven very useful ...fluorescent sensors. Retroviral vector background Until the development of viral vectors, gene transfer into mammalian...un-transfectable cells, labs use viral vectors and particularly retroviral vectors because they target a broader...restricted to in vitro cell culture as lentivirus-derived vectors are capable of transducing certain quiescent...generation of lentiviral vectors consisted of three plasmids encoding 1) the lentiviral vector genome which was...

  7. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...building block Entry vectors and 5 lentiviral Destination vectors. These vectors can induce combinatorial... Viral vector topic page Beginners guide to viral vectors Quick guide to all things lentivirus Resources...several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated...research purposes, retroviral vectors have been developed into ‘split-vector systems’ by separating viral...and expression of these vectors when used in experiments. Retroviral vectors make up a large toolbox ...Gamma-retroviral vs. lentiviral vector systems Gamma-retroviral vectors have a relatively high transduction... many more useful viral vectors available at Addgene: Follow our viral vector blog posts for updates on...

  8. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ... While lentiviral vectors are popular gene delivery tools, producing lentivirus, can pose certain challenges...Producing lentivirus: it's all about the T's (transfection and titers) To produce lentivirus, the plasmids...producing lentivirus, it is time to titer. One important consideration when titering a lentivirus is the ...FAQs answered Tips for titering your lentivirus Visit the viral vector featured topic page Resources on ...Addgene.org Lentiviral Guide Find lentivirus plasmids Browse all viral vectors ...challenges associated with producing and using lentivirus and offer some tips and tricks for overcoming...generation The first step in delivering genes via lentivirus is deciding what packaging system is best for...

  9. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...of producing CRISPR lentivirus. As we’ve heard from scientists, producing lentivirus is not always an easy... your own lentivirus stocks. Our research scientists have provided their standard lentivirus production... things in science, getting a high titer Cas9 lentivirus prep takes a bit of time and effort that, for...easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert) and the reduced titer associated...associated with large inserts can make lentivirus production frustrating - a researcher can complete an entire...providing lentiviral preps of select lentiviral vectors from the repository for you. We perform test infections... There are multiple CRISPR tools available as lentivirus and we’ll be adding more as the need arises. ...

  10. The AAVantages of AAV in CRISPR Screens

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    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... screens. AAV vectors can be made at high titers equal to those achieved with lentivirus but offer an ... while both AAV and lentivirus have low immunogenicity, AAV still beats lentivirus. In mice, AAV is almost...that is unmatched by other viral vector delivery methods. Lentivirus has limited packaging options, with...packaging capacity and stable expression of lentiviral vectors make them an attractive option. However, safety... particularly in vivo ones. In those cases, AAV vector screens may be AAVantageous!       In vivo, in ...expression in many mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect...experiments. AAV can be safely handled at BSL-1, while lentivirus requires a BSL-2 setup. This requires a less ...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...Viral Vectors 101: Viral Vector Elements Viral Vectors 101: An Introduction to AAV Viral Vectors 101:... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...gamma-retrovirus, coming in around 9 kb. Unlike gamma, lentivirus can infect both dividing and non-dividing cells...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...

  12. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...Lentiviral vectors are one of the most popular and useful viral vectors in the lab. Advantages of lentivirus...dividing and non-dividing cells. Lentiviral vectors are the vector of choice for many CRISPR applications,...and future) applications of lentiviral vectors! Lentiviral vector history and components In the early ...viral vector systems based on retroviruses like Moloney murine leukemia virus (MMLV). The vectors could... to the lentivirus HIV-1, well-known to infect non-dividing cells. The first lentiviral vector system ...adeno-associated viral vectors can also target non-dividing cells, lentiviral vectors can carry much more...Lentiviral Plasmid FAQs. Lentiviral vectors in the lab Lentiviral vectors are incredibly popular in the lab...

  13. Viral Vectors 101: Pseudotyping

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    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...lentiviral vector will infect, pseudotyping can help. In pseudotyping, you produce viral vectors using viral...such as lentivirus, retrovirus, and rabies virus. How to pseudotype To pseudotype your viral vector, you ...Sandoval S (2017) Pseudotyped Lentiviral Vectors: One Vector, Many Guises. Human Gene Therapy Methods ...To deliver genes using lentiviral vectors, you need an envelope protein on the virus’s surface and a ...you use the same protocol for generating lentivirus, but you use a different envelope glycoprotein instead... lentiviral particles.   Why pseudotype? Lentiviruses and retroviruses typically target immune cells...Sometimes this is due to the cells that the viral vectors were produced in. For example, production of vesicular...

  14. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    Published
    Aug. 6, 2024, 1:15 p.m.
    ...about γ-retroviral vectors How do engineered γ-retroviruses differ from lentiviruses? There are a few important...Addgene blog Viral Vector Elements The Retroviral Life Cycle Popular Retroviral Vectors and Their Use in... cells a virus can infect. Note: While both lentiviruses and γ-retroviruses use the same gene products...differences between engineered γ-retroviruses and lentiviruses. First, these viruses were derived from different...genomes (MoMLV and MSCV for γ-retrovirus; HIV for lentivirus). Additionally, γ-retroviruses can only transduce...during mitotic breakdown of the nuclear envelope. Lentiviruses can transduce both dividing and non-dividing...terminally differentiated (non-dividing) cells. Both lentiviruses and standard γ-retroviruses use the Gag, Pol...

  15. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    Published
    March 15, 2016, 2:30 p.m.
    ...Resources on the Addgene Blog Read Our Lentivirus FAQ Learn How Lentivirus is Used in CRISPR Genome-Wide Screening...prepped your DNA, transfected and harvested your lentivirus. Now it’s time to move ahead with your infection...primers that target the common features of viral vector backbones; once validated these universal primers...transfection; if preparing several different lentiviruses in parallel, this approach should be considered...higher than for functional assays depending on the vector backbone. When using qPCR based methods for viral...plasmid which can over-inflate results. A packing vector deficient in reverse transcriptase will produce...the number of target cells that are positive for vector encoded transgene expression following transduction...

  16. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...Service Suggestion Form. Lentivirus As some of the most popular lentiviral vectors in the repository are ...Expanding the viral vector knowledge base: You can help! We’ve been expanding our viral vector educational resources...spending time and money producing virus from select vectors in the repository yourself, you can now order ready-to-use... of our new Viral Service, we’re distributing lentivirus (with many CRISPR tools included among the preps... production process while still ordering viral vectors. Virus production can be a frustrating process ...we’ve got viruses to help you out. Of course, lentiviruses are used in experiments other than just those...those involving CRISPR and so we also have lentivirus for delivering control shRNAs, GFP, and rtTA from ...

  17. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    Published
    May 13, 2025, 1:15 p.m.
    ...lentiviral vectors, how they work, and why you might choose them over integrating lentiviral vectors.  Before...integrase-deficient lentiviral vectors, let’s briefly revisit the basic biology of lentiviral vectors (for a more detailed...lentiviral vectors that are competent for one round of infection (AKA transduction in the viral vector world...life cycle of a lentiviral vector with an integrase-deficient viral vector. Created with BioRender.com...integrase-deficient lentiviral vectors? Now let’s turn to integrase-deficient lentiviruses (IDLVs). As the name ...lentiviral vectors? There are several reasons why you might choose an IDLV over a lentiviral vector: 1. No...include detecting vector particle components or performing quantitative PCR titration of vector DNA in samples...

  18. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ... on the Addgene blog Viral Vectors 101: Types of Viruses Viral Vector Elements Tips for Preventing... contact with than GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular...cellular function, this would also be a higher risk vector. While it is unlikely that you, or anyone else ...viruses grown in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...humans, earning this virus a BSL-2 classification. Lentivirus (a type of retrovirus) and gamma retrovirus require...and required in BSL-2 labs. When working with lentivirus, many organizations require or recommend disposable...you have to work with BSL-2 materials, such as lentivirus. Most of this work will need to be done in a ...

  19. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...cells Viruses AAV and lentivirus Baculovirus; can produce AAV and lentivirus if needed # of plasmids...Overcoming the Challenges of Lentivirus Production Tips for Titering Your Lentivirus Preps More resources on...fast-growing, and widely available. The viruses Lentiviruses, AAV, and adenoviruses can all be produced in...cells. However, it is much more common to make lentiviruses and AAV in-house. High-titer adenoviruses are...labs or companies. Production of both AAV and lentivirus requires three plasmids — a transfer plasmid ... (three packaging plasmids for 3rd generation lentivirus production). The packaging plasmids are widely...AAV has a packaging capacity of ~4.7 kb, while lentivirus has a capacity of 8–10 kb. Time Viral production...

  20. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...use viral vectors for many experimental purposes. To help you make sense of all the viral vector information...Subscribe to Viral Vector Blog Posts from Addgene Need more information on viral vectors? Check out our ...our viral vector guide pages, our Lentivirus FAQ, and our viral service pages. Click on the Image Above ...most important characteristics of retroviruses, lentiviruses, AAVs, and adenoviruses in this easy-to-use ... viral vector...

  21. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...HEK cells allows production of lentivirus. Purification of this lentivirus results in the pooled library...our protocol on Lentivirus Production provides a general approach for producing lentivirus in Lenti-X 293T... need to worry about amplifying and producing lentivirus. But pre-made libraries aren’t quite plug-and-play...transduce your cells.  Titering After generating your lentivirus, you’ll want to infect your cells with the virus–the... received your pre-made library/produced your lentivirus. Since viruses are sensitive to freeze/thaw cycles...

  22. Viral Vectors 101: Viruses as Biological Tools

    Type
    Blog Post
    Published
    March 21, 2023, 1:15 p.m.
    ... Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ... Viruses are essentially DNA (or RNA) delivery vectors, so what makes them better than the alternatives...measures in a separate, upcoming post. If a viral vector seems like the right approach for your experiment...viruses used in mammalian research: 𝛄-retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV)...mitosis. The most well-known retrovirus is HIV. Lentiviruses are a genus of the retroviral family that can...Immunogenicity Insertional Mutagenesis Possible Lentivirus Infects non-dividing cells Insertional...experiment?           Click Here to Find Viral Vector Resources at Addgene! References and Resources...

  23. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...modular vector assembly process designed for a quick, plug-and-play approach to cloning CRISPR vectors (McGee...for each module, generate the vector file, and quickly clone their vector of interest. And it truly is ...destination vectors for various delivery methods, along with compatible inserts. All destination vectors contain...heavy lifting out of CRISPR vector design, making it easier to get the vectors that are just right for your...Fragmid destination vectors currently available. Table 1: Fragmid destination vectors Organism Delivery...Delivery Type Destination Vector Mammalian Lentivirus pRDA_512 (RSV-driven; lentiGuide-based...designing a CRISPR vector the Fragmid way!    Figure 1: Selecting your vector type on the Fragmid...

  24. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...Production at Addgene Viral Vectors 101: What is a Virus? Viral Vectors 101: Viral Vector Elements ..., a viral vector is selected to perform the transduction with: either retrovirus, lentivirus, adenovirus...desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic acid will be...produced, and viral particles are packaged, when the vector is expressed. Once the design and construction ... it’s time to make virus! The constructed viral vector containing the cargo of interest is then typically...layers of risk management when working with viral vectors. Typically, one day after transfection into the...variety of broadly applicable and popular viral vectors. Check out our viral selections to see if we have...

  25. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...different types of tubes: the ones we use to store lentivirus and the ones we use to store AAV. Our results...effect on the pH of the samples stored in our lentivirus tubes (Fig. 1). However, once thawed, there was...protocol for recombinant adeno-associated virus vectors. Mol Ther Methods Clin Dev. 2014 Aug 13;1:14034... 1st Time CRISPR User) Tips for Titering Your Lentivirus Preps Additional Resources on Addgene.org Check...Find Virus Protocols Browse Our Viral Vectors ... lentivirus...

  26. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...generate lentivirus (B). Cas9-expressing cells or wild-type cells are treated with lentivirus containing... Quick Guide to All Things Lentivirus Get Tips on Titering Your Lentivirus Plasmids 101: NGS Quality Control...genome wide-screening experiments commonly use lentivirus to deliver a pooled population of gRNAs to Cas9...population of gRNA-containing lentiviral transfer vectors, each targeting a specific gene within the genome...gRNAs are then cloned into lentiviral transfer vectors to create the CRISPR library. CRISPR libraries ... concentration that is sufficient to generate lentivirus. Be sure to use next-generation sequencing to...the steps above! Cells are then transduced with lentivirus containing the CRISPR library to generate a heterogeneous...

  27. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...expression vectors which are often more difficult to transfect or package into viral vectors. Cas9 multiplexing...Cpf1 multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence... 1) Most rely on transfection of more than one vector to express the gRNAs and Cas9. Co-transfections...multiplexing vectors are larger because they require regulatory sequences to allow for expression of multiple...Yamamoto Lab Golden Gate Assembly transfection One vector expresses Cas9 and up to 7 gRNAs Each gRNA requires...Expression Cassettes lentiviral transduction One vector expresses Cas9, eGFP, and up to 4 gRNAs Each gRNA... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA...

  28. Viral Production at Addgene

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    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core. Transfections...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles...our virus protocols. Browse our AAV vector collection. Lentivirus Production Lentiviral preparations ...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...method.  Titering by the University of Pennsylvania Vector Core is (as of April 2016) also performed by ddPCR...

  29. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    Published
    July 2, 2019, 12:51 p.m.
    ...Neuron-optimized viral vectors Alternatively, Cas9 activators can be delivered to the CNS by lentivirus, which has...lentiviral vectors for in vitro and in vivo CRISPRa (Savell et al., 2019). This dual lentivirus system pairs...including transgenic mice, neuron-optimized viral vectors, and high-throughput screening approaches. Here...largely precludes delivery by adeno-associated vectors (AAV). This issue was recently circumvented by ... ubiquitous expression of sgRNA(s) and mCherry (vector 1) with neuron-specific expression of dCas9-VPR... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...BioRender.com. The Day Lab used their optimized vectors to highlight a key advantage of CRISPRa over traditional...

  30. Viral Vectors 101: The AAV Retrograde Serotype

    Type
    Blog Post
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    April 27, 2023, 1:15 p.m.
    ...-dependent vectors, this can cause challenges for the experimental design as these vectors can exhibit...your system. Chemogenetic vectors  Expression of retrograde serotype vectors is present at both the injection...serotype to conduct experiments with chemogenetic vectors, this property must be taken into account since...projection neurons retrogradely transduced by the vector. One method for minimizing the impact of this phenomenon...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection pathway...then inject a recombinase-dependent chemogenetic vector directly into the region where the cell bodies ...Addgene Blog Get Started with AAV Learn more about Lentivirus Find out More about Addgene's Viral Service ...

  31. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...blog Viral Vectors 101: Viral Vector Elements Viral Vectors 101: Types of Viruses Viral Vectors 101: Viral...developed into retroviral vectors and 2.) a subclass of retroviruses called the lentiviruses (like HIV-1) — that...that have been developed into lentiviral vectors. Lentiviruses are sometimes referred to as “complex retroviruses...would not be great for a gene therapy vector! For viral vectors, only the protein for reverse transcriptase...components of the vector particle, some of the RT protein gets packaged inside of the vector particle, along...and this is where viral vectors and natural viruses diverge. For the viral vector, its job as a tool is ...seem like an unlikely candidate to use as a viral vector, but retroviruses have been developed into some...

  32. Delivery Methods for Generating iPSCs

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    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...so either a single polycistronic vector or multiple single-gene vectors can be used to package reprogramming...reprogramming vectors available from Addgene. Another drawback is that lentiviral vectors insert in different...Cre-lox lentiviral vectors: This approach is the same as using a standard lentiviral vector, but transient...Non-integrating iPSC delivery methods Viral Vectors Adenovirus: Adenoviral vectors infect dividing and nondividing...reprogramming factors: Mouse Expression Vectors Human Expression Vectors Carey et al Hockemeyer et al Brambrink...transgene reactivation, residual expression Lentivirus Integrating High Low Efficient and Stable Genome...Excisible Medium Medium Generates transgene-free and vector-free cells Genome integration Need to sequence...

  33. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...Viral Vectors 101: Viral Applications Viral Vectors 101: Viruses as Biological Tools Viral Vectors 101:...create far safer viral vectors that can be used in BSL-1 or BSL-2 labs. Viral vectors are viruses that have...increase the viral vector’s carrying capacity. In order to allow the viral vectors to reproduce when experimentally...expressed via cells cultured with the vectors. This allows the viral vectors to reproduce only in experimentally...: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral vectors are, it...of Viral Vectors 101 posts about each type, so be sure to check our blog, or our Viral Vectors 101 eBook...viral particle is called a virion. Finally, viral vectors, which are used in the lab, are distinct from viruses...

  34. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and your gRNA of interest...lentiviral titer as compared to previous lentiviral vectors. It can be used both to knock-out single genes ...backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR v2 is key ...is available from Addgene both as a single empty vector and in pooled libraries containing thousands of...Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide libraries for CRISPR screening....

  35. Important Considerations When Using AAVs

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    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ... AAV vectors without specific facilities (Biosafety Level-1). Other possible tools, lentiviruses for example...Immune Response: Compared to other viral vectors (e.g. Lentivirus, Adenovirus) AAVs cause the least amount...-associated viral vectors: packaging capacity and the role of rep proteins in vector purity." Human gene...Cas9 and AAV Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research...Future? I knew little about adeno-associated Viral Vectors (AAVs) before starting my graduate program at the...in locomotion. We have relied heavily upon AAV vectors to deliver chemogenetic/optogenetic constructs ...Although they have a small packaging capacity, AAV vectors were suitable for our needs for the following reasons...

  36. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...My lab's vector of choice is AAV, with nearly every experiment requiring AAV. Before joining my lab, ...always make more AAV than I think I need. Unlike lentivirus, AAV requires a higher number of viral particles...Production of recombinant adeno-associated viral vectors for in vitro and in vivo use. Current protocols...Addgene.org Learn how Addgene produces AAV and lentivirus Check out Addgene's virus protocols Find AAV ...

  37. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...Browse Our Viral Vector Pages Learn About Viral Vector Biosafety Deposit Your Viral Vectors ...We offer a variety of viral vectors, viral services and even a Viral Vectors eBook to get you started. ... terrains. They are, in fact, a choice delivery vector from basic science research all the way to clinical...is more than one type of virus to choose from (lentivirus, retrovirus, AAV, etc.). When you’ve decided ... long-term, and even inducibly depending on the vector and virus. Viral CRISPR-Cas9 tools have been...interest, turning expression off permanently. Viral vectors can also be used to express proteins. Viral expression...of interest, and often a selectable marker. This vector can express your construct in target cells transiently...

  38. Viral Vectors 101: Biosensors

    Type
    Blog Post
    Published
    July 6, 2023, 1 p.m.
    ...engineered into viral vectors, so if you want to use them, you’ll need to use the vectors (or redesign the ...ready to deliver your sensor, whether you choose lentivirus, gamma retrovirus, or AAV as your vehicle, we...Biosensors Viral Service: Biosensor AAV Viral Vectors 101 eBook Resources on the Addgene blog An Overview...

  39. 15 Years of Addgene: The Top 15 Plasmids

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    Blog Post
    Published
    Jan. 8, 2019, 1:54 p.m.
    ...  Find the scrambled shRNA vector. pLKO.1 - TRC cloning vector - This vector from the David Root lab is...our CRISPR posts Read our viral vector blog posts Read our lentivirus guide Resources on the Addgene ...opening our UK office in 2014, to starting our viral vector service in 2016...we’re excited to help scientists... be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such as our...lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used to deliver Cas9...your gRNA of interest into mammalian cells. This vector was the top requested plasmid of 2015. What makes... This plasmid from the Zhang lab is a mammalian vector that expresses both SpCas9 and your gRNAs and was...

  40. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    Published
    June 24, 2020, 5:45 p.m.
    ...methylation and activate transcription. A lentiviral vector with the same modifier, Fuw-dCas9-Tet1CD, is available...Jaenisch’s lab in plasmid form or as ready-to-use lentivirus. Figure 2: dCas9-Tet1 demethylates cytosines...with the former also available as ready-to-use lentivirus. Figure 3: dCas9-DNMT3A methylates cytosines... targeted enhancers. Although the lab used this vector in chick embryos, it also functions in mammalian...and this fusion allows for packaging into viral vectors and targeted delivery into hard-to-manipulate cell...targeting to a wider set of genomic targets. These two vectors are part of the larger pMVP/pMAGIC cloning system...
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