We narrowed to 118 results for: Cre

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

Learn about the top requested AAV from 2017: pmSyn1-EBFP-CRE, a modular tool for expressing Cre-recombinase in neurons.

Jan. 17, 2018, 2:57 p.m.

Learn how site specific recombinases like Cre-lox and FLP-FRT can be combined for complex control over gene expression in neuroscience experiments and more.

Oct. 19, 2017, 1:54 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Learn how the Gradinaru Lab at Caltech developed AAV vectors to target the peripheral and central nervous systems.

Sept. 28, 2017, 2:01 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

FLEx vectors utilize Cre/Lox to conditionally manipulate gene expression in vivo.

April 28, 2016, 2:30 p.m.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

July 11, 2019, 1:26 p.m.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Aug. 6, 2019, 12:55 p.m.

Learn about new plasmids at Addgene including AAV split base editors, Ace2-mScarlet voltage indicator, CyanoGate MoClo cloning toolkit, and CreLite.

March 3, 2020, 2:15 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.

The Open Repository of CRISPR Screens is a resource to increase the accessibility of CRISPR screen data. Learn how to use this for your research.

Jan. 21, 2020, 2:30 p.m.

Learn about items from Addgene's viral service including lentiviral preps expressing SARS-CoV-2 proteins, ChR2 for targeted expression, DREADDs, GFP dependent Cre and FLPo, and more.

May 28, 2020, 1:15 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Learn how FLEx and optogenetics combined can be combined to control gene expression with high spatial and temporal resolution.

Oct. 17, 2019, 12:28 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

Learn how you can use retrograde AAV for neuronal tracing.

April 27, 2023, 1:15 p.m.

Learn about new and upcoming viral vectors from Addgene including our new trial size AAVs for serotype testing, calcium and GABA sensors, and more!

Sept. 12, 2019, 1 p.m.

Gamma-retroviral vectors and lentiviral vectors can be used in a wide variety of experiments including those involving genome engineering, RNAi, and inducible gene expression. In this post, learn about some of the most popular viral vectors available from Addgene and their uses.

July 24, 2018, 12:51 p.m.