We narrowed to 22 results for: Flp recombinase

...post). Optimizing FLP FRT through smart engineering Different FLP versions – making FLP efficient The first...fellow SSR system FLP-FRT. In addition, a variety of means to spatiotemporally control FLP and Cre expression...Read on to learn more about FLP-FRT, Cre-lox, and how combinations of FLP and Cre enable additional levels...of genetic control. What is FLP FRT and how does it work? FLP recombinase, which is named after its ability...Sadowski 1985). The FLP system works in a similar fashion to the Cre system. The FLP recombinase recognizes FRT...(LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...fusions allowing Cre/FLP translocate to the nucleus where they exert their recombinase activity (Logie and...

...orientation specificity of the site-specific recombinases (SSRs) Cre and FLP. SSRs bind DNA at target sites to induce...specific recombination events: Cre recombinase binds loxP sites, while FLP binds FRT sites. When a DNA sequence...orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA fragment...pair of FRT sites and include both Cre and FLP recombinases.   Figure 1 (modified from Schnutgen...absence of, or mCherry in the presence of Cre recombinase.   Once the SSR of choice is introduced, ...events are impossible even in the presence of Cre recombinase. This plasmid now specifically drives expression...about a Revolution: The Impact of Site-Specific Recombinases on Genetic Analyses in Mice. 2004. PubMed PMID...

... using multiple recombinases. To make multiple, precise genome edits, the recombinases used must have ...determine the cross-reactivity of these recombinases, each recombinase and reporter construct pair were tested...however, the other recombinase/reporter pairs were inactive, even at very high recombinase levels. These data...toxicity of these recombinases by crossing flies homozygous for a UAS-driven recombinase with flies heterozygous... For Dre, Flp, KD, R, B2 and B3, approximately 50% of offspring carried both the recombinase and GAL4,... Rubin’s work shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila ...recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald Rubin’s lab...

... an alternative to the conventional Cre or Flp recombinases used to excise markers. It has the advantage...

...occur. Cre recombinase can be used alongside common, existing tools for fly research that use Flp recombination...Drosophila melanogaster and is under the control of Flp recombinase. The XFPs in Flybow are membrane-tethered, ...

...-responsive CreERT, the coupling of Cre-lox with FLP-FRT recombination to generate additional recombination...Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different promoters, Brainbow...143(1):134-44. PubMed. Drosophila Brainbow: a recombinase-based fluorescence labeling technique to subdivide...

... EGFP Recombinase AAV Finally, we are excited to add Flp-expression AAV to our recombinase AAV inventory...offer a Flp-expressing AAV, as well as a Cre-dependent Flp-expressing AAV. See our recombinase AAV inventory...inventory. Flp is another useful recombinase that can be used in combination with Cre for intersectional...detection PSAM4 GlyR for chemogenetic inhibition Flp expression for intersectional studies And more! ...inventory here New recombinases Plasmid Serotype Name 55637  AAVrg pAAV-EF1a-Flpo 87306  AAV1 AAV... µL size serotype testing) pAAV-hSyn-EGFP Recombinases Plasmid Serotype Name 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...

...components derived from the P1 bacteriophage: the Cre recombinase and a loxP recognition site. The P1 bacteriophage...components for use in genome manipulation. Cre recombinase, originally named because it “causes recombination...although later referred to as the “cyclization recombinase”), is a 38 kDa protein responsible for intra-...How does it work? As alluded to above, the Cre recombinase catalyzes the site specific recombination event...such as with tamoxifen or doxycycline), the Cre recombinase can be expressed only in specified cells or at...in a single system. The Saccharomyces cerevisiae FLP-FRT recombination system is another site-directed... conceptually similar to Cre-lox, with flippase (Flp) and the short flippase recognition target (FRT) ...

...pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan Roth   Recombinases Recombinase systems such as Cre-lox allow for more...types, recombinases allow you to further restrict gene expression to areas in which the recombinase and ...expression. Plasmid 75470 is now available in AAV1 for Flp-dependent expression of ChR2(H134R) bound to an mCherry...and construct are coexpressed. Three new recombinases from the Cepko Lab are now available as AAV1. CRE-...expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469), is also available in AAV1. Plasmid...pAAV-EF1a-C-CreintG Connie Cepko 75469 AAV1 pAAV-EF1a-Flp-DOG-NW Connie Cepko Viral vectors coming soon...

...models that express recombinases like Cre or Flp in their cells of interest and recombinase-dependent AAV vectors...recognized by Flp), to either flip or excise a gene that is located between them. Early recombinase-dependent...system is off-target or spurious expression of recombinase-dependent AAV vectors. In this article, we will...minimize it. Overview of DIO/FLEx vector design Recombinase-dependent vectors utilize pairs of specific sequences... to prevent transcription in cells where the recombinase was not present (Kuhlman, SK. 2008). While theoretically...spurious expression?   Figure 1.  Cre-recombinase binds to either pair of lox sites, let's say ...This spontaneous recombination persists even in recombinase deficient E. coli and under conditions used to...

...inventory of recombinases and recombinase-dependent vectors. We recently released several recombinases in various...Calcium sensors and GABA sensors Optogenetics Recombinases And more! Control AAV For many AAV experiments...pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting and manipulating specific, genetically-defined...collection. Of note, we are excited to release a Flp-dependent Cre AAV in AAV9 and AAVrg. See our new ...Dre vector AAV phSyn1(S)-DreO-bGHpA (50363-AAV5) Flp vectors pAAV-EF1a-mCherry-IRES-Flpo (55634-AAV1)...

...including our first far-red fluorescent marker, a Flp-dependent channelrhodopsin in the retrograde serotype...New serotype pENN.AAV.CMVs.Pl.Cre.rBG AAV5 Recombinase James M. Wilson New serotype AAVrg = AAV ...

...phenomenon is to use a retrograde recombinase-expressing vector (e.g. Cre or FLP) to transduce the projection...only become activated in the presence of Cre recombinase, which is limited to the hippocampus. Thus, only...titers for efficient retrograde transport. For recombinase-dependent vectors, this can cause challenges ...projection pathway neurons and then inject a recombinase-dependent chemogenetic vector directly into the region...

...viral vector is used to deliver DNA encoding Cre recombinase under the control of the synapsin promoter. It... neurons or you might use it in combination with Flp-FRT. Additionally, you could use the retrograde form...

...pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both Cre and Flp dependent. Plasmid Serotype Name 124603 AAV9...our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several new options...mCherry)-EGFP 50457 AAV2 pAAV-hSyn-DIO-EGFP Recombinases Plasmid Serotype Name 87306 AAVrg, AAV5...

...developed three new versions. Flipbow employs the Flp recombinase/FRT system in place of Brainbow’s Cre/loxP,...all-in-one versions which express self-excising Cre recombinase from the same transgene as the Brainbow cassette...

...genetic tools: activators, inhibitors, sensors, recombinases, and more. With the help of adeno-associated...classical genetic tools, for example Cre/loxP or Flp/FRT, with AAV vectors are common approaches to control...

...Chimeric channels for neuronal manipulation Recombinases Plasmid Serotype Name 50363  AAV5  AAV ...)-DreO-bGHpA 121675  AAV9  pAAV-EF1a-fDIO-Cre (FLP-dependent CRE) 55634  AAV1  pAAV-EF1a-mCherry-IRES-Flpo...

... is achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined... "Adeno-associated virus 2/9 delivery of Cre recombinase in mouse primary afferents." Scientific reports...recombination. Purification and properties of the Cre recombinase protein." Journal of Biological Chemistry 259.3...

.... We have expanded our recombinase-dependent virus selection to now include Flp-dependent DREADDs viral...