We narrowed to 25 results for: RFP

In the second episode of our Hot plasmids podcast, learn about new RFPs, AAV tools, and plasmids for generating transgenic zebrafish.

Sept. 8, 2017, 7:52 p.m.

Fluorescent biosensors can be used to measuring changes in autophagic activity over time. Several autophagy biosensors, such as Rosella, Keima, and tandem fluorescent proteins are described.

Jan. 22, 2019, 2:41 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

April 10, 2018, 12:56 p.m.

Two techniques developed by Addgene depositors have made it possible to track mRNA translation at the single molecule level using fluorescent proteins.

Aug. 1, 2017, 1:15 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2017.

Jan. 9, 2018, 3:02 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Addgene's plasmid kits allow you to order many plasmids for a single purpose at a low price.

Sept. 14, 2016, 2:30 p.m.

Overview of recombinases that can be used as a Cre-alternative for site-specific recombination in Drosophila.

May 12, 2015, 1:32 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

Tips on how to choose compatible fluorescent proteins for multi-color imaging.

Oct. 9, 2014, 3 p.m.

Photoactivatable fluorescent proteins can be switched on (and sometimes off) enabling a variety of techniques and applications.

April 25, 2017, 2:30 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

Learn how you can use new photosensitizers to specifically kill target cells.

Sept. 19, 2017, 1:20 p.m.

Rosella is a fluorescent biosensor that you can use to study autophagy.

April 13, 2017, 2:30 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

This round-up of new plasmids from Addgene include blue-light inducible Cre for use in bacteria, CRISPR-Casɸ mGold, and new CRISPR plasmids and viral preps.

Jan. 5, 2021, 2:15 p.m.