We narrowed to 33 results for: Streptococcus

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

March 10, 2020, 1:15 p.m.

Learn about using SpyLigase for irreversible peptide-peptide ligation and magnetic cancer cell capture.

Aug. 13, 2014, 3:49 p.m.

A quick guide on the main components of CRISPR: the Cas9 endonuclease and the guide RNA.

Feb. 2, 2016, 5 p.m.

This post highlights ways to overcome the AAV size limitation to deliver CRISPR editing tools to cells.

Sept. 16, 2020, 1 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

Dec. 18, 2018, 1:17 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Jan. 11, 2017, 8:47 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

Dec. 17, 2019, 2:37 p.m.

Learn the basics behind generating generating mouse models using CRISPR/Cas9.

July 12, 2016, 2:30 p.m.

The Doudna lab recently described a new, thermostable Cas9 variant, GeoCas9. Learn how you can use GeoCas9 in your next genome engineering experimetn.

Sept. 14, 2017, 12:40 p.m.

An introduction to Gram negative and positive bacteria - what are they, how can you tell the difference, and where did this terminology come from?

Aug. 2, 2022, 1:15 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Learn about some newly developed Cas proteins and how to choose a Cas protein based on site accessibility, specificity, and sensitivity.

Nov. 5, 2019, 1:28 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Find the latest plasmids and viral vectors to available at Addgene in the October 2020 hot plasmids blog post!

Oct. 13, 2020, 1:15 p.m.

Use anti-CRISPR proteins to turn off genome editing and prevent off-target effects. In this article, read about how it works and its applications

July 23, 2020, 1:20 p.m.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Jan. 10, 2017, 3:37 p.m.

Take a look at the developments in genome engineering throughout history and the parallels in science fiction.

Feb. 25, 2020, 2:15 p.m.

Learn about how CRISPR systems are classified. Addgene's guide to typing CRISPR.

March 18, 2025, 1:15 p.m.