We narrowed to 28 results for: Streptococcus

1. What's New in CRISPR - March 2020

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   ... derived from Streptococcus pasteurianus  or the type II CRISPR3 module of Streptococcus thermophilus ...thermophilus (the popular Streptococcus pyogenes system does not work well in Caulobacter). To test their CRISPRi...

2. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

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   ...SpyCatcher system, based on a protein isolated from Streptococcus pyogenes that locks itself together, to produce...they call SpyLigase (Spy comes from the “S” in Streptococcus and the “py” in pyogenes), capable of locking...links in the chain.  Addgene: What about the Streptococcus pyogenes protein domain made it ideal for this...

3. Components of CRISPR/Cas9

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   ... Species/Variant of Cas9 PAM Sequence Streptococcus pyogenes (SP); SpCas9 3' NGG SpCas9 D1135E...Neisseria meningitidis (NM) 3' NNNNGATT  Streptococcus thermophilus (ST) 3' NNAGAAW  Treponema ...

4. What's New in CRISPR - Winter 2018

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   ...control of CRISPR-Cas9. By combining AcrIIA4, a Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor...

5. Overcoming the AAV Size Limitation for CRISPR Delivery

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   ...infection of certain tissues. However, packaging Streptococcus pyogenes (SpCas9) and a gRNA together (~4.2 ...Cas9 include the use of St1Cas9 (~3.3 kb) from Streptococcus thermophilus and a rationally-designed truncated...

6. The PAM Requirement and Expanding CRISPR Beyond SpCas9

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   ...below.  Cas9 species PAM sequence ’) Streptococcus pyogenes (Sp) 3' NGG Staphylococcus aureus... Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus (St) NNAGAAW Treponema denticola...

7. Your Top Requested Plasmid in 2016!

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   ...types. Purpose  SpCas9 This plasmid expresses Streptococcus pyogenes (Sp) Cas9 from a Cbh promoter. Cbh ...

8. What's New in CRISPR - December 2019

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   ...deaminase (TadA) to a catalytically impaired Streptococcus pyogenes Cas9. They called this editor Target-ACE...

9. Generating Mouse Models Using CRISPR/Cas9

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   ...system derived from Streptococcus pyogenes. An alternative Cas9, from Streptococcus aureus, is as effective...

10. Some Like it Hot: Thermostable GeoCas9

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    ...mesophilic (medium heat loving) bacteria, such as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t ...

11. Microbiology Resources

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    ...Salmonella sp. Shigella sp. Staphylococcus sp. Streptococcus sp. Synechococcus sp. Thermus sp. Vibrio sp....

12. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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    ...sequence targeted by the Cas9 nuclease. The Streptococcus pyogenes Cas9 (SpCas9) requires an NGG PAM to...

13. Plasmids 101: Gram Negative and Positive Bacteria

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    ...species of bacteria. The stain colored one type, Streptococcus pneumoniae, while the alcohol used in the staining...

14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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    ... the most frequently used Cas9, spCas9 from Streptococcus pyogenes, this sequence is 3’-NGG. But Cas9s...

15. 15 Years of Addgene: The Top 15 Plasmids

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    ...plasmid from the Zhang lab expresses Cas9 from Streptococcus pyogenes with an invariant sgRNA scaffold and...

16. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

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    ... repression in the Gram-positive bacterium, Streptococcus pneumoniae. Robert Husson lab's M. tuberculosis...enzyme. They identified the Cas9 orthologue from Streptococcus thermophilus, Cas9sth1 as a robust and highly...

17. Hot Plasmids - October 2020

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    ...Addgene Sc++ is a codon-optimized, engineered Streptococcus canis Cas9 nuclease that has a broad 5′-NNG-...

18. Truncated gRNAs for Regulating Gene Expression

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    ...conventionally used SpCas9 (derived from the bacterium Streptococcus pyogenes). Consequently, fewer genetic tools...

19. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

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    ...meningitidis (Nme) (Pawluk et al., 2016). While Streptococcus pyogenes Cas9 (SpyCas9) is the most used and...

20. A History of Genome Engineering in Popular Culture

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    ...Frederick Griffith used mouse models to prove that Streptococcus pneumoniae bacteria could be transformed from...