We narrowed to 701 results for: plasmid

Learn more about how to verify your plasmid via a diagnostic restriction digest.

Aug. 28, 2014, 3:34 p.m.

Plasmid incompatibility is the inability of different plasmids to be maintained in a cell. We’ll cover why this happens and how it might affect your experiment.

March 31, 2020, 1:15 p.m.

New to plasmids? Learn the basics of plasmids in this blog post and find out about the parts of a plasmid, how they are made, and how scientists can use them in the lab.

April 2, 2020, 6:17 p.m.

Not getting enough DNA from your plasmid preps? This post outlines variables like endA and low copy number that could affect DNA yields and suggests steps to super-charge your plasmid preps.

May 26, 2016, 2:30 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

Tips for naming your plasmid in a way that is clear and informative to you and your colleagues.

Feb. 13, 2014, 1 p.m.

Learn about the components of a plasmid page on our website and shows how the different components can be useful to you

Feb. 4, 2016, 3:30 p.m.

Addgene answers questions about lentiviral plasmids, including 2nd vs 3rd generation transfer vectors, packaging plasmids, biosafety concerns, and more.

April 23, 2014, 1:08 p.m.

22 of the interesting and popular hot plasmid technologies of 2014 from Addgene's plasmid repository.

Jan. 6, 2015, 5:21 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Find tips on how to perform both large and small scale plasmid verification and sequencing.

Jan. 14, 2016, 3:30 p.m.

Can't find a plasmid at Addgene? Suggest new plasmids for the repository by tweeting at us of filling out our "Suggest A Plasmid" form here.

March 16, 2016, 5:30 p.m.

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

April 19, 2017, 3:25 p.m.

Read about Gladys Kaba, the 3rd recipient of the Addgene/Seedinglabs Plasmid Grant, who will be using plasmids as positive controls in cervical cancer screens.

Nov. 8, 2016, 3:30 p.m.

Learn how you can use the Broad Target Accelerator Plasmid Collections from to help enable precision cancer medicine research.

May 11, 2017, 2:30 p.m.

Addgene and Seeding Labs recently awarded plasmids to two researchers in Ghana. Read about their work inside.

June 15, 2016, 1:30 p.m.

Addgene Board Member Connie Cepko gives us the scoop on progress in retinal gene therapies and the development of GFP-activatable tools in her lab.

Dec. 8, 2015, 3:30 p.m.