We narrowed to 621 results for: Dos
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TypeBlog PostPublishedFeb. 26, 2019, 1:40 p.m....opportunities to fall in your lap. Sometimes, in order to do the thing you love, you have to create opportunities...honest), I would tell them to follow your passion. Do what you love. Don't worry about the rest.”- Jason...
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CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....maybe smaller Cas systems could exist in nature. To do this, they mined a database of microbial genome and...indiscriminately cleave ssDNA in trans like Cas13 and Cas12 do for RNA and dsDNA, respectively. However the authors... -
Making Career Development an Integral Part of the Science Training System
TypeBlog PostPublishedFeb. 25, 2021, 2:15 p.m....Unfortunately, many science graduate programs are still doing an inadequate job of supporting science trainee ...into diverse, successful careers. We’re not even doing a very good job of providing skills training for... -
Save Time and Money by Making Your Own Competent Cells
TypeBlog PostPublishedJan. 28, 2014, 3:10 p.m....Addgene, so we need to be cost and time efficient. We do this by making our own competent cells and using ...each new batch of competent cells, don’t forget to do a negative control (plate your cells without adding... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....of proteins functioning in a variety of ways. How do scientists focus on the activity or function of a...plasmids here! Where should I place the tag and how many do I need? When designing a plasmid containing a polyhistidine...perform assays that they otherwise would not be able to do. We hope that this blog has answered all of your ... -
Giving gRNAs a Facelift - Synthetic and Beyond
TypeBlog PostPublishedOct. 20, 2022, 1:15 p.m.... synthetic modifications, specifically ones which do not contain a 5’ triphosphate in the gRNA, reduce...many suitable dyes to choose from and you can opt to do-it-yourself with a kit or purchase it commercially...but you can still choose what dye to use What to do once you’ve chosen a gRNA and modification(s) Congratulations... -
RNA Extraction Without A Kit
TypeBlog PostPublishedMarch 24, 2020, 1:15 p.m....without a kit that outlined several reasons why doing something without a kit has advantages: less plastic...without saying that one should take care whenever doing any type of DNA or RNA purification to avoid contamination...expression. There are several things that researchers can do to combat this issue. First and foremost, be mindful... -
The Breast Cancer Microenvironment: A Tumor’s Backstage Team
TypeBlog PostPublishedJan. 31, 2019, 1:32 p.m....cells - a rich soup of life encircling the tumor. How do microenvironment enzymes and metabolic reprograming...mediators and improving cancer cells proliferation. How do microenvironments affect tumor spread and drug resistance...for a tumor to spread and to resist drugs. And to do that, let’s take a look at exosomes. Proficient in... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m....fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...just a question you have about the world. You might do some background research to better understand it ...method to problems in a variety of contexts. How do you develop problem solving skills? Thankfully, as... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....you have all this knowledge, the question is: How do you know what’s too much (or too little)? The answer...things aren't working out, adjust the ask. I used to do a lot of outreach to student groups and being aware...think about everything you’re asking your learners to do, it becomes a lot easier to build successful, accessible... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m.... little more about how I ended up at pgEd, what I do there, and how you can set yourself on a path towards... affiliation, and ethnic or personal identity. We do this through a variety of activities, including creating...newspapers, or starting your own blog. Even if you do not end up pursuing a career in science communication... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m.... plans to raise salaries. What are institutions doing after the injunction? We are particularly interested... have found out about what their institution is doing from Future of Research over social media, not from...recommendations, even when a federal mandate encourages them to do so. Immediately after the injunction, as we started... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog PostPublishedDec. 8, 2016, 3:30 p.m....procedure dramatically reduces the number of clones that do not contain the recombinant plasmid and therefore...are identified much more efficiently, as those that do not contain the desired insert should not grow. ...be present. Most common cloning strains of E. coli do not contain the F plasmid (and are considered F-)... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....experimental questions, but not having the tools needed to do so. Cepko lab researcher Jonathan Tang wanted to ...mouse lines expressing GFP in specific cell types to do more than just label cells. If GFP could be co-opted...fluorescent proteins dsRed, mCherry, and TdTomato do not induce transcription. Thus, T-DDOGs can be used... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....technologies and spaces where interested parties can do research. This interest manifested itself in the ... are on the lookout for genome modifications that do not exist in the CGC library but that would be useful..., we have members with a wealth of skills to help do science on the cheap using equipment that would otherwise... -
Kwabena Duedu on Public Health, Biofuels, and Doing Science in Ghana
TypeBlog PostPublishedDec. 20, 2016, 3:30 p.m....visited Cambridge to spend some time learning and doing research at Novartis. Listen to learn how Kwabena... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....unlikely you’ll be able to do them all for every western blot assay you run. How do you know which steps to...visualize them with a conjugated antibody. If you do not need such precise measurements, you can use a... double ladder on the lefthand side. Whatever you do, be consistent and document it. Did it work? Save...directly from tissue or supernatant, and you’re doing the purification and cross-adsorption in lab, consider...adjusting your exposure time first, since you can do that without having to re-run the western. Remember...the data and what level of accuracy you’ll need to do that confidently. Once you’ve run your western, ... -
Top Requested AAV of 2017: pmSyn1-EBFP-CRE
TypeBlog PostPublishedJan. 17, 2018, 2:57 p.m....Additional Resources AAV Titers: Where do they come from and what do they mean? AAV: A Versatile Tool for... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m.... Reddit AMA Here are some concrete things you can do to develop those transferable skills while you are...relies on a division of labor Serve on a committee and do something big like plan a conference Recruit many... -
Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein
TypeBlog PostPublishedJune 28, 2018, 3:01 p.m....csRNP method is as simple as doing routine PCR assays. Here is a simple to-do-list to follow when planning...Medicine (CRM), Edinburgh. Most commercial antibodies do not work in pull-down assays: Epitope tagging provides...knock-in efficiency (<2% positive by ICC for the tag) or do not see any cells positive for tag insertion, you...et al. 2018), however, if you have any questions, do not hesitate to contact me at [email protected]...