We narrowed to 617 results for: Dos

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

If you’re using CRISPR to make a genome edit, how do you know if your experiment was successful? Try using fluorescent CRISPR reporters like SRIRACCHA and GEmCherry2.

May 26, 2020, 1:15 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

Learn about the Reproducibility for Everyone collaboration and how you can bring reproducibility workshops to your conference or university.

Feb. 28, 2019, 1:34 p.m.

New CRISPR pooled libraries, such as SAM, CRISPRa, and CRISPRi, offer new ways to do genome-wide functional screens.

Feb. 24, 2015, 7:50 p.m.

The advantages of participating in a peer mentoring group for scientists and how to form your own.

Feb. 18, 2014, 4:22 p.m.

Get free science-themed Zoom backgrounds from Addgene! Backgrounds include plasmids, viruses, DNA, and more.

April 22, 2021, 1:15 p.m.

Learn about the Professional Development Hub's work to support science trainee career transitions. Register for the event covering findings from their report!

Feb. 25, 2021, 2:15 p.m.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Jan. 28, 2014, 3:10 p.m.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

Oct. 19, 2023, 1:15 p.m.

Considering purifying a protein to study it? Learn all about the polyhistidine tag, or HIS, in this blog post!

March 2, 2023, 2:15 p.m.

An overview of gRNA modifications available to CRISPR users and some of the advantages of using synthetically modified guides in your experiments.

Oct. 20, 2022, 1:15 p.m.

What are we asking of our audience when we tell them about our science? Cognitive load explains how your learners have to work too.

June 16, 2022, 1:15 p.m.

In this post from our Transferable Skills series, learn how problem solving is a core part of your research training as well as a valuable skill for your future career.

Nov. 30, 2021, 2:15 p.m.

Find tips for working with RNA and simple protocols for extracting RNA without using a kit

March 24, 2020, 1:15 p.m.

Learn about new RNA-targeting Cas13d enzymes that average only 2.8 kb in size, are easy to package in low-capacity vectors, have high on-target and low off-target editing rates.

May 3, 2018, 1:48 p.m.

Future of Research Executive Director, Gary McDowell, explains how evolving wage regulations are impacting universities' decisions to change postdoc salaries.

Jan. 11, 2017, 3:30 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

Connie Cepko's lab at Harvard University has developed transcription factor and CRE recombinase variants activated by GFP. Learn more inside!

Nov. 24, 2015, 3:30 p.m.

Makerspaces provide a place for self-directed research. Learn how members of a Makerspace in Victoria Canada model worm locomotion.

July 23, 2015, 2:30 p.m.