We narrowed to 228 results for: cloning
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TypeBlog PostPublishedMay 26, 2020, 1:15 p.m.... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...
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Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog PostPublishedMarch 3, 2020, 2:15 p.m....Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....bi-allelic mutations typically desired when screening clones for complete functional knockout of your target... -
Hot Plasmids - February 2022
TypeBlog PostPublishedFeb. 22, 2022, 2:15 p.m....popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing... -
Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers
TypeBlog PostPublishedJuly 28, 2015, 2:30 p.m....event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m....removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector... -
Addgene: The First Twenty Years
TypeBlog PostPublishedDec. 19, 2024, 2:15 p.m....Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... additionally provides detailed information on cloning procedures used to construct the deposited plasmids... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m.... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids... -
Plasmids 101: Terminators and PolyA signals
TypeBlog PostPublishedMarch 31, 2016, 2:30 p.m....Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog PostPublishedSept. 24, 2015, 2:30 p.m....evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ... -
Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria
TypeBlog PostPublishedApril 4, 2019, 12:53 p.m....Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005... -
10 Basic tips for mammalian cell culture
TypeBlog PostPublishedJuly 12, 2018, 1:09 p.m....the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m.... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding... -
PCR Applications for Copy Number Variation Assays
TypeBlog PostPublishedApril 26, 2022, 1:15 p.m.... primer Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll... -
Plasmids 101: Degron Tags
TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m.... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based...