We narrowed to 236 results for: cloning

Addgene scientists perform standard quality control for AAVs. But we are also going one step further with next-generation sequencing (NGS) of the viral genome.

Sept. 12, 2017, 1:44 p.m.

Get tips on how to monitor your protein of interest inside cells - co-localization, complex formation, and more.

June 22, 2017, 2:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

Learn how Cpf1 stacks up against Cas9 and NgAgo

July 14, 2016, 2:30 p.m.

Learn about new tools in Addgene's collection, including new biosensors for neuroscience research, plasmids for a common lab enzyme, and more!

May 5, 2026, 1:15 p.m.

Learn about the advantages of using AAV as a delivery method for CRISPR screens.

Feb. 4, 2025, 2:15 p.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

Curious about the history of C-myc, one of the most common antibody tags? Learn all about it here!

Jan. 19, 2023, 2:15 p.m.

Find plasmid tools for COVID-19 research expressing viral open reading frames, host factors, and tools for detecting SARS-CoV-2 and generating pseudoviruses.

July 12, 2021, 1:15 p.m.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

Feb. 5, 2019, 1:04 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Learn how and why Adeno-associated virus (AAV) has enabled breakthroughs in retinal gene therapy.

July 31, 2018, 12:55 p.m.

Fluorescent protein timers can be used to determine molecular age. Learn how to use these unique tools in this post.

May 4, 2017, 2:30 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Cpf1, a newly discovered Cas9 homolog, is smaller than Cas9, can target different sequences, and may more efficiently generate targeted mutations.

Oct. 14, 2015, 2:30 p.m.

Learn about TnpB, a small genome editor with a function similar to CRISPR systems!

July 29, 2025, 1:15 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

Learn about RNA interference and how you can use shRNA plasmids to knockdown gene expression!

June 17, 2025, 1:15 p.m.

This guide on single chain fragment variables (ScFvs) covers how ScFvs are made, the advantages and disadvantages of using ScFvs, and several applications.

June 3, 2021, 1:15 p.m.