We narrowed to 235 results for: cloning

...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...

...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...

...evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target...

...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ...

...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...

... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids...

... primer  Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...

...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids...