We narrowed to 235 results for: cloning

...Additional Resources on Addgene.org  Yeast-mediated cloning CRISPR plasmids: Yeast Yeast Synthetic Biology...

... lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5 kb of insert...

...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...

...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...

... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...

... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids...

...evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target...

...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ...

.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...