We narrowed to 234 results for: cloning

...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...

...biology. There are already two comprehensive plant cloning kits available in the repository, MoClo and GreenGate...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

... You can use DNA sequencing or other molecular cloning techniques to determine CRISPR/sgRNA efficiency...

...Article contributed by Andrew Hempstead The Modular Cloning (MoClo) system allows synthetic biologists to use...

...et al. Check out Addgene's Lentiviral dTAG cloning vector and SMASh degron tagging vector! Destabilizing...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...event. Now it’s maybe one out of 100 or 50 event. Cloning of the second plasmid can be done in one day in...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...Addgene for the basics of molecular biology and cloning, since they have reasonable introductions to lots...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

... easy to edit, and easy to share. However, the cloning process — including the propagation of plasmids...

... additionally provides detailed information on cloning procedures used to construct the deposited plasmids...

...Promoter Controls Gene Expression Learn about New Cloning Techniques Read all of our Plasmids 101 Posts ...

...evaluate component delivery and sgRNA efficacy by cloning in genome target sequence into EGFP reporter. Target...

...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ...

... primer  Molecular Biology Reference Molecular Cloning Techniques References Bell AD, Usher CL, McCarroll...

... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based...

...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...