We narrowed to 20 results for: cloning

Protocol for Ligation Independent Cloning

Protocols for plasmid cloning by PCR.

Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Modify your plasmid MCS or add short sequences using oligo overlap cloning.

A wide variety of protocols from Addgene that can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and more.

Protocol, tips, and FAQ for how to perform a restriction digest of plasmid DNA

Get started with Gibson Assembly Cloning!

Protocol - How to Ligate Plasmid DNA

Protocol for how to purify DNA from an agarose gel, including tips to help improve the resolution and separation of bands.

A collection of Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos.

Description of Polymerase Chain Reaction with protocol, tips and FAQ

This protocol describes how to generate a monoclonal cell line from a polyclonal pool of stable cells.

Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol.

Tips on designing primers for PCR

Learn how to transform E. coli with your plasmid of interest.

Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and separation of bands.

Use this protocol to prepare LB agar plates with antibiotic in your lab.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.