We narrowed to 236 results for: cloning

FastCloning is a fast and easy technique that can replace restriction cloning. Learn about this great plasmid cloning technique here.

Nov. 1, 2016, 2:30 p.m.

PCR can be used to append the appropriate restriction sites to your insert and facilitate downstream cloning. Read on for tips on this process.

March 29, 2016, 2:30 p.m.

Scientists are not limited to restriction enzyme cloning. Gateway cloning is a rapid and efficient way to move DNA into multiple vector systems.

Jan. 12, 2017, 3:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Quickly isolate mammalian cells clones using the agarose cloning method.

Sept. 7, 2017, 12:17 p.m.

Use CcdB to enhance the efficiency of your next plasmid cloning experiment.

Dec. 8, 2016, 3:30 p.m.

Restriction cloning is the cloning technique most molecular biologists start off with. Learn the ins and outs of restriction cloning here.

Feb. 18, 2016, 3:42 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Oct. 27, 2016, 2:30 p.m.

Looking for a way to test a lot of different parts in your cloning, or maybe interrogate some complex genetic circuits? MoClo just might be the approach for you!

March 12, 2024, 1:15 p.m.

In this troubleshooting guide, find step-by-step tips and tricks for troubleshooting your cloning experiment.

Sept. 24, 2019, 12:55 p.m.

Sequence and ligation independent cloning (SLIC) lacks the strict requirements of many standard cloning techniques. Learn how to use SLIC in this post.

Sept. 26, 2024, 1:15 p.m.

We describe the different modular cloning kits available for different applications and organisms to help you find the kit that's right for your experiment.

May 14, 2024, 1:15 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

Here, we cover four different plasmid screening strategies including blue-white screening, colony PCR, restriction digest analysis, and Sanger sequencing.

Sept. 15, 2020, 1:15 p.m.

There are many plasmid tools available in molecular biology so how do you chose the right cloning method for you? Here, consider speed, cost, difficulty, efficiency, and the number of inserts when designing your cloning strategy.

Aug. 21, 2018, 12:31 p.m.

Learn about the best tips and tricks for modular cloning (MoClo) of plasmids!

July 8, 2025, 1:15 p.m.

Gibson Assembly is a popular cloning alternative to traditional restriction/ligation methods. Learn more about this technique here.

March 1, 2016, 3:30 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.