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We narrowed to 62 results for: cloning

Showing: 1 - 20 of 62 results

  1. DNA Service - Cloning Grade DNA

    Type
    Collection
    ...directly transfect cloning grade DNA into cells? Cloning grade DNA is designed for use in cloning applications... analyses are performed on my cloning grade DNA order? For each cloning grade DNA prep, the plasmid sequence...like to see available as cloning grade DNA, please let us know using the Cloning Grade DNA Suggestion Form... ordering cloning grade DNA for over 200 of our most popular plasmids! DNA... DNA Service: Cloning Grade DNA ...Details Plasmids Available FAQ Addgene is offering Cloning Grade DNA (cgDNA) for over 200 plasmids in our ...variety of popular plasmids and backbones with high cloning potential. By making these plasmids available as...

  2. Synthetic Biology - Cloning and Genomic Tools

    Type
    Collection
    ...related to Cloning and Genomic Tools for library construction, genomic modification, and cloning systems....Biology plasmids for cloning and genomic tools. Plasmid... Plasmid Collections Synthetic Biology Cloning and Genomic Tools...Tools Synthetic Biology: Cloning and Genomic Tools SynBio Resources SynBio Home Browse All SynBio Assembly...Assembly Standards SynBio Blog Genome Engineering Cloning & Genomic Tools Metabolism Networks & Gene Regulation...systems. Jump to Table: Cloning and Genomic Tools Reporters and Tags Library Construction Integration Recombination...Recombination Shuttle Vectors Plasmids for Cloning and Genomic Tools Search the table by keyword or sort by...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...CRISPR/Cas9 Clones for Deletions and Clone Selection For suspension cells, transfer all clones to a single...deletion clones with a proofreading polymerase and clone the amplicons ( e.g., with a PCR cloning kit) into...one plate to allow for clones to grow and use the other plate to screen each clone for deletions. Add an...Validation of Biallelic Deletion Clones In order to characterize obtained clones and validate a successful knockout...present a simple methodology for CRISPR design, cloning, and delivery for the production of genomic deletions...within exons will enrich even monoallelic deletion clones for loss of function. This is due to the high frequency...complement including additional nucleotides for cloning and expression purposes. NOTE: Here we discuss ...

  4. CRISPR References and Information

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    Collection
    ...gRNA design and cloning gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs... Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo... Fly: gRNA cloning pAc-sgRNA-Cas9 PDF, 169 KB Marraffini Bacteria: pCas9 new spacer cloning pCas9 PDF,...Lander gRNA cloning into pLX-sgRNA pLX-sgRNA PDF, 129 KB Sontheimer gRNA design and cloning All-in-one ... helps design (10 different prediction scores), clone (primer design), and evaluate gRNAs, as well as ...plasmids PDF, 107 KB Chen and Wente Zebrafish: gRNA cloning, in vitro transcription, injection gRNA core ; ...PDF, 110 KB Goldstein Nematode: gRNA design and cloning Cas9-sgRNA construct PDF, 364 KB Goldstein Nematode...

  5. Luciferase Plasmid Collection

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    Collection
    ... of interest are cloned into a Firefly luciferase reporter vector via Gateway cloning to test for the ...Renilla and Firefly luciferase. Plasmids are Gateway cloning compatible. Learn more about DULIP in our 27 Hot...collection of empty backbone plasmids into which you can clone your regulatory element or gene of interest into... of 5' promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc...Creation of N-terminal Nanoluc fusions using Gateway cloning Mikko Taipale 87075 pLenti6.2-ccdB-Nanoluc NanoLuc...Creation of C-terminal Nanoluc fusions using Gateway cloning. Lentival. Mikko Taipale 87078 pLenti6.2-Nanoluc-ccdB...Creation of N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly...

  6. Plant Plasmids and Resources

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    Collection
    ...Scott Lenaghan. Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional...as the promoter or cloning method of choice. ID Plasmid Description Promoter Cloning Method PI Return to...Protein Guide Addgene Blog: Plant Biology CRISPR Cloning Transcription Factors RNAi Empty Backbones Fluorescent...Vladimir Nekrasov. The MoClo-compatible zCas9i Cloning Kit includes a high efficiency intron-optimized...plants (including rice and wheat) - Caixia Gao. Cloning The following collections are designed for creating... rescue in plants - Martin Parniske. GreenGate Cloning System to create plant expression vectors containing...constructs - Johannes Stuttmann. MoChlo: Modular Cloning Chloroplast Toolbox for design of synthetic operons...

  7. Zhang Lab CRISPR Page

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    Collection
    ...Protocols 2013). For cloning information, please view: Zhang Lab General Cloning Protocol 237.2 KB SpCas9...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...annealed oligos (design is indicated below) can be cloned scarlessly into the vector before the sgRNA scaffold...annealed oligos (design is indicated below) can be cloned into the CRISPR array. The oligos are designed ...activator helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...for insertion of guide sequences Zhang Lab SAM Cloning Protocol 321.5 KB Return to top AAV - In vivo Genome...

  8. Brzezinski Lab CRISPR Collection

    Type
    Collection
    ...Additional Cloning Information Cloning Protocols Single guide cloning: Zhang Lab General Cloning Protocol...provided as empty backbones that utilize Golden Gate cloning to insert the desired guide RNA sequence by digestion...Protocol (PDF, 237 KB) Dual guide cloning: Brzezinski Lab Dual Guide Protocol (PDF, 990 KB) Plasmid and Sequence...

  9. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... mutant clones from watermelon have been codon-optimized for expression in bacteria and cloned into an...region of MDH. For detailed clone information, please see the MDH Protein Clone Information Sheet, available...malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research experience...IPTG-inducible His-tag expression vector. All His-tags are cloned at the C-terminus of MDH and, if indicated on the...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd gen lentiviral backbone for cloning and expression ...may contain tags or fusion proteins, a multiple cloning site (MCS), an inducible promoter, and/or a selectable...with C-terminal mTagBFP2 tag pNIC-GST-TEV-GG - Cloning of target gene with N-terminal GST tag and TEV ...for bacterial expression, Ligation Independent Cloning (LIC) Also see our Bacterial Expression collection...zebrafish insulin promoter vector with multiple cloning sites Browse our full Fluorescent ...thioredoxin) Protein purification pNH-TrxT - LIC cloning in bacteria with N-terminal His-TrxA-TEV tag NusA...

  11. Microbiology Resources

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    Collection
    ...- Barbier Lab Cloning and Expression Bacteria and yeast expression vectors : FX cloning System - Geertsma...Parts Kit : Molecular cloning for E. coli - Densmore Lab EcoFlex MoClo : Modular cloning for applications ... and Dutzler Labs EasyClone 2.0 Yeast Toolkit - Borodina Lab Golden GATEway cloning system - Wittbrodt...Microbiology : Sets of plasmids for fluorescence, cloning and expression, and synthetic biology. CRISPR Resources...sets of plasmids intended for use together (e.g. cloning and expression systems) and therefore supplied ...

  12. Antibody Plasmid Collection

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    ...well as by our trusted partner labs. Learn More Cloning and Expressing Antibodies Antibody Plasmids Antibodies...synthetically produced recombinant antibodies, created by cloning antibody components into plasmids and expressing...Monoclonal antibodies are derived from an individual clone targeting a single antigen. An example of R-mAbs...and their applications in our Antibody Guide ! Cloning and Expressing Antibodies The following table highlights...mammalian expression vector A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep... Rep. 2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals...

  13. TALEN Plasmids and Kits

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    ...336 pFusX clones allowing for Golden Gate assembly of 15-16 RVD TALEN inserts in one cloning step rather...Stephen Ekker Golden Gate TALEN 2.0 Golden Gate cloning method. Validated in multiple organisms. Dan Voytas...Keith Joung Zhang Lab TALE Toolbox PCR/Golden Gate cloning method. Optimized for human expression. Feng Zhang... Assembly Kit Assembly by ligation-independent cloning (LIC). Validated in human cells. Veit Hornung Musunuru...plasmids sequence positions 1214–2210 of pTAL3 were cloned into a pCS2 expression vector resulting in shorter...RR) used are obligate heterodimers that require cloning of left and right TALEN monomer proteins into opposite...enhancers in mammalian cells. These plasmids allow for cloning DNA fragments encoding TAL effector repeat arrays...

  14. Synthetic Biology - Overview

    Type
    Collection
    ...Proteins FREQ-Seq Kit FX Cloning Kit GreenGate Cloning System (Plant) MoClo Modular Cloning Modular PUF Assembly...Plasmids by Category Cloning and Genomic Tools Browse plasmids related to cloning and genomic modification...

  15. CRISPR Plasmids - Tagging

    Type
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    ...currently unavailable, you will need to design and clone in a gRNA(s) to guide the Cas9 protein to your target... target sequence, as well as design and clone in the homology arms for the donor plasmid. The Mendenhall... also provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB... complexes. This vector serves as a backbone to clone the left and right homology arms. More details on...al. Cell Rep. 2015 . Alternatively, cDNAs can be cloned directly into this vector and targeted to the AAVS1...include Cre and FLP expression vectors, a general cloning plasmid, and a prebuilt Unc-32::GFP targeting vector...

  16. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...promoter. WPRE has been removed to increase the cloning capacity. pCDH-CB-IRES-copGFP-T2A-Puro 72299 Express...well as the Cre and Flp recombinases. Genes can be cloned into these transfer vectors using shuttle vectors...pAdx-CMV-LacZ is uniquely suited to simplify the cloning process as it can be used in blue-white screening...Xgal/IPTG plates — if your insert is successfully cloned into this vector, transformants will instead form...

  17. Lentivirus Plasmids

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    Collection
    ....1 – TRC cloning plasmid 3rd U6-driven shRNA empty plasmid; includes a stuffer for easy cloning Root 14748...shRNA. See article for detailed information about cloning & additional plasmids. Aebischer and Trono 11795...cassette (promoter and gene of interest) can be cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha... the gene of interest, also can express shRNA (cloning H1-shRNA cassettes into the unique SnaBI site in...

  18. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... for Cloning 67720 Worm NotI/BamHI none S. pyogenes Colaiacovo pRB17 52527 Fly blunt end cloning none ...or nick. Selection , such as Puromycin or EGFP Cloning enzyme used for insertion of your gRNA sequence... by the Doudna Lab . Plasmid Expression System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection ...BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI none S. pyogenes ... U6 yes, cut (enhanced) S. pyogenes Zhang gRNA Cloning Vector Bbs I ver. 2 85586 Mammalian BbsI None S...nickase expression cassette using the Golden Gate cloning method. A separate accessory pack is available ...expressed from independent promoters. sgRNAs are cloned into separate vectors and then assembled into one...

  19. Open Enzyme Collection

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    ...collection include polymerase chain reaction (PCR), cloning, isothermal amplification, reverse transcription...the basis of manufacturing their own enzymes by cloning them into an expression vector of their choice ...not functional by themselves. Researchers should clone these enzymes into an expression vector of their...Plasmids Jennifer Molloy Lab Plasmids Golden Gate cloning...

  20. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...The original green fluorescent protein (GFP) was cloned in 1992 ( Prasher et al., Gene, 1992 ), and since...506 517 93 5.7 10 min Monomer pCS2+mNeonGreen-C Cloning Vector - Mammalian Expression Jump to Top Yellow...529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C) - Mammalian Expression pCS2+8CmCitrine.../Xenopus/C.elegans/Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1... 548 562 49 6.5 2.5 hr Monomer pET mOrange LIC cloning vector (u-mOrange) - Bacterial Expression pCS2+...pmScarlet_C1 - Mammalian Expression pCS2+mScarlet-C Cloning Vector - Mammalian Expression mScarlet-I 569 593...587 610 16 4.5 15 min Monomer pcDNA3 mCherry LIC cloning vector (6B) - Mammalian Expression pLV-mCherry ...
Showing: 1 - 20 of 62 results