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Showing: 181 - 200 of 455 results

  1. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    Published
    March 8, 2017, 3:15 p.m.
    ...depositing scientist, you will have the most involvement with Step 1, and, while data entry can be a chore...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...

  2. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...intend to be productive with the granting agency’s investment. 6. Failure is part of the process, keep your...applications there’s going to be an element of luck involved so it’s important not to throw in the towel if...

  4. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...approaches.  Nanoblades can also be used to non-invasively gene edit the liver of adult mice. When mice ...generating transgenic mice strains and for non-invasive in vivo gene editing. The bottom line on Nanoblades...

  6. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    Published
    Aug. 6, 2019, 12:55 p.m.
    ...you want combined in your offspring. Genotyping involves getting a small piece of tissue from each mouse...effectively breeding your mice. Because their generation involves the integration of a novel gene randomly into ...

  7. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in non-human primate (NHP...This new capsid will be a valuable tool for investigating NHP brains. Figure 3: Overview of library...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...flexibility of the single-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying... viral preps available!  We are expanding our inventory of ready to use viral preps from the Deisseroth...

  9. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ... of outcomes including excision or insertion, inversion, translocation or cassette exchange. By utilizing... clade of huge phages. In this article, they investigated three Casɸ orthologs, Casɸ-1, Casɸ-2, and Casɸ...

  11. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... factor What is fun for the audience? Getting involved and feeling "closer" to the panel. Your audience...incorporate them into the program. I have to admit to inviting interesting friends and colleagues to events for...

  12. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...well, and they thought we could be taught. They invested in us this summer – $30,000 for research and another...flower that changes color, but the experience is invaluable. This opportunity has given us the chance to ...

  13. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...give Gateway a try. This method, developed by Invitrogen and based on lambda phage integration sequences...Wouldn’t it be nice if after all the hard work involved with cloning, you would at least be guaranteed...

  14. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...into multiple bacterial species including several involved in human disease. However, the rate of transfer...for the study of essential genes such as those involved in antibiotic resistance. Are you ready to move...

  15. Tips for a 1st time AAV user (by a Rookie AAV user)

    Type
    Blog Post
    Published
    Oct. 23, 2018, 11:49 a.m.
    ...autoclaving, which also takes time. Rather than investing all of this effort in preparing reagents, I choose...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human gene therapy ...

  16. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...many many times in a short time period. PCR was invented in the early 1980s by Kary B. Mullis who later...to design PCR primers.  Types of PCR Since the invention of PCR, different PCR methods have been developed...

  17. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...recommend using primers targeting the viral genome or inverted terminal repeats (ITR). You should always have...quantification of adeno-associated virus serotype 2-derived inverted terminal repeat sequences. Human Gene Therapy ...

  18. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...that change single base pairs, to chromosomal inversions and large deletions. Many of these methods rely...However, there is the chance of a chromosomal inversion, or just random mutations if the chromosome is...
Showing: 181 - 200 of 455 results