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A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

Aug. 10, 2017, 2:19 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Dec. 16, 2015, 3:30 p.m.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

Jan. 10, 2023, 2:15 p.m.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

April 6, 2023, 3 p.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

An in-depth look at fixing and permeabilizing samples in immunofluorescence experiments.

Aug. 30, 2022, 1:15 p.m.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Learn some best practices to keep your mammalian cell lines free of contaminants and your experiments running smoothly.

July 12, 2018, 1:09 p.m.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

May 29, 2018, 1:51 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how to use the pPSU plasmids to generate affordable DNA ladders.

July 14, 2017, 2:30 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Aug. 28, 2025, 1:15 p.m.

Addgene's antibody lab member Kate describes each step in recombinant antibody production process.

Aug. 13, 2024, 1:15 p.m.

Ever wondered how Addgene makes our viral preps? We explain it all here!

Oct. 24, 2023, 1:15 p.m.