We narrowed to 396 results for: %s

An overview of non-antibody affinity reagents such as Fab fragments, diabodies, and single-chain fragment variables

April 14, 2022, 1:15 p.m.

Get advice on tagging optogenetics and chemogenetics receptors to ensure that your receptors express and/or localize to the membrane.

May 11, 2021, 1:15 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

A paper published in Nature used CRISPR to correct a disease-causing mutation in human embryos. Does this paper herald a future of human germline editing?

Aug. 10, 2017, 2:19 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

Use CRISPRainbow to label multiple genome sites with fluorescent proteins of various colors.

Feb. 28, 2017, 3:30 p.m.

Rationally engineered Cas9 variants eSpCas9 SpCas9-HF1 enhance CRISPR on-target specificity. Learn how in this post.

Dec. 16, 2015, 3:30 p.m.

Long-read sequencing has allowed researchers to identify plasmid dimers and multimers. Learn about them - and how to troubleshoot - in this blog post.

Jan. 10, 2023, 2:15 p.m.

When you have multiple antibodies to choose from, does isotype matter? Yes, and understanding how can help you pick the right antibodies for your experiment.

April 6, 2023, 3 p.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Use the deposit spreadsheet to quickly deposit multiple plasmids with Addgene.

March 8, 2017, 3:15 p.m.

This post provides an overview on how transcription terminates in prokaryotes and eukaryotes.

March 31, 2016, 2:30 p.m.

Learn about the most common injection routes in mice, including their key differences and uses.

Jan. 22, 2025, 9:42 p.m.

An in-depth look at fixing and permeabilizing samples in immunofluorescence experiments.

Aug. 30, 2022, 1:15 p.m.

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Learn how rabies virus can be used to trace neuronal connections. AAV available at Addgene can help you with the process!

May 29, 2018, 1:51 p.m.

Learn about xCas9, an engineering Cas9 variant with broad PAM flexibility and high on-target specificity.

March 28, 2018, 6:52 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn how to use the pPSU plasmids to generate affordable DNA ladders.

July 14, 2017, 2:30 p.m.