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We narrowed to 231 results for: cloning

Showing: 201 - 220 of 231 results

  1. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

  7. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    Published
    Feb. 4, 2025, 2:15 p.m.
    ... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like...

  9. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...

  11. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal...

  13. Lab to Office Culture Shock

    Type
    Blog Post
    Published
    Feb. 5, 2019, 1:04 p.m.
    ... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

  14. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...

  17. Cpf1: A New Tool for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Oct. 14, 2015, 2:30 p.m.
    ...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

  20. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    Published
    Jan. 29, 2019, 2:27 p.m.
    ...SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial expression vectors to create a library...
Showing: 201 - 220 of 231 results