We narrowed to 234 results for: cloning

...developed for another decade when in 1988 they were cloned by two separate labs (Bird et al., 1988; Huston...an scFv?  scFv sequences have traditionally been cloned by amplifying the VLand VH antibody sequences of...

... al., 2017). Our vectors have two Bsa1 sites to clone the single guide RNA (protospacer+scaffold) in a...single polycistronic tRNA-gRNA (PTG) gene system to clone multiple protospacers which enables us to target...

...followed by BbsI restriction sites into which you can clone your 20 nucleotide guide sequence of choice (for...

...cell lines is to transfect, select, and isolate clones. This procedure is cumbersome and inefficient, ... somewhere between one hundred and one thousand clones to find a cell line with the edit of interest. ...

...These plasmids allow for a gene of interest to be cloned into the pSNAP-tag (m) Vector or pCLIP-tag (m) ...

...target species, target name, host organism, isotype, clone details, vendor, method, tissue preservation, target...

...plasmids The transfer plasmid is the vector you clone your gene of interest into and is an important feature...Lentiviral production and transduction Once you have cloned your gene of interest into lentiviral transfer ...

... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution...

... Then, your desired DNA to be integrated can be cloned into the vector that you have selected. The nucleic...

...of non-plasmid DNA or heterogeneous and/or mixed clones present in the original sample. At seqWell, we ...

... To use this SEC-based system, first design and clone 500-700 bp homology arms for your gene of interest...

...into your functional experiments! Whether you’ve cloned the plasmid yourself or obtained it from a colleague...

...truncated versions of SARS-CoV-2 S (spike protein) cloned into a CMV driven expression vector are also available...

...popularity of FLAG and HA tags, you may not even need to clone new constructs to label your favorite protein! While...

...bi-allelic mutations typically desired when screening clones for complete functional knockout of your target...

...removal – in conventional mouse targeting, targeted clones are selected for using a resistance marker; however...

...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

...C234-E25: attB2r-SNAP-attB3 SNAP-tag Gateway entry clone to add C-terminal SNAP-tag Promega Plasmid Collection...

...RNAi. Here, a short sequence targeting the gene is cloned into a viral shRNA expression vector and the vector...

...software and synthesized. Pooled gRNAs are then cloned into lentiviral transfer vectors to create the ...