We narrowed to 236 results for: cloning

Learn about CRISPR plant base editors for plants

Jan. 3, 2019, 1:35 p.m.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

June 23, 2016, 2:30 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

The Addgene repository has many amazing plasmids used by labs all over the world, but what plasmid was request most in 2015?

Jan. 11, 2017, 8:47 p.m.

Vectors containing the piggyBac transposon are now available at Addgene. Learn how these unique tools can be used for genome engineering!

May 31, 2016, 3:30 p.m.

This post highlights some of the tools Addgene makes available to industry scientists.

Jan. 13, 2026, 2:15 p.m.

Learn what non-model organisms are, why scientists use them, and what they can teach us.

July 21, 2026, 1:15 p.m.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

March 21, 2017, 2:30 p.m.

Learn about the IBEX Knowledge-Base, which allows researchers to share validation data on imaging reagents for IBEX imaging.

June 8, 2023, 1:15 p.m.

Get an overview of flow cytometry including how it works, what you can use flow cytometry for, and tips and tricks for a successful flow cytometry run.

July 20, 2021, 1:15 p.m.

Transducions use viral vectors to insert nucleic acids into cells. Learn all about them in our Viral Vectors 101!

April 30, 2024, 1:15 p.m.

Addgene is now using NGS services from seqWell Inc for plasmid validation. Learn how the seqWell sequencing process works here.

April 19, 2017, 3:25 p.m.

This blog post describes a a self-excising cassette that can be used with CRISPR/Cas9 tools in C. elegans to generate genomic modifications.

July 7, 2015, 3:36 p.m.

Learn more about how to verify your plasmid via a diagnostic restriction digest.

Aug. 28, 2014, 3:34 p.m.

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Feb. 22, 2022, 2:15 p.m.

Ginkgo Bioworks and Addgene are synthesizing and distributing thousands of plasmids for COVID-19 research. Read to learn more about these constructs

May 19, 2020, 1:15 p.m.

Overview of Cre-lox recombination and how it is used to make DNA modifications in transgenic animals, embryonic stem cells, and tissue-specific cell types.

Jan. 13, 2015, 3:47 p.m.

A deep dive into qPCR with a focus on analysis, chemistries, and bench tips.

Sept. 29, 2022, 1:15 p.m.

Learn about click biology and how you can use it to advance your experiments and explore biological phenomenon.

Oct. 21, 2025, 1:15 p.m.

Check out how you can use digital PCR to validate your CRISPR genome edits.

March 30, 2017, 2:30 p.m.