We narrowed to 234 results for: cloning

...the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with the Agarose Method Plasmids...

...Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding...

...Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005...

...Type: Choose an empty backbone if you intend to clone your own insert, or use the other options to find...

... (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination vector with SapI...

... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005...

.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies...

...protocols and guides regarding pooled libraries, from cloning a pooled library to viral production to screening...

... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like...

... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal...

...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome...

... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...

...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...

...control elements for each would be relatively large. Cloning the viral genes in this plasmid would be more difficult...

...yeast strain that also contains a reporter gene cloned downstream of a promoter containing the target ...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

... gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors...