We narrowed to 235 results for: cloning

...Type: Choose an empty backbone if you intend to clone your own insert, or use the other options to find...

...Kyoung-Hee, et al. "A Tn7-based broad-range bacterial cloning and expression system." Nature methods 2.6 (2005...

... (Fig. 1, Step 1). There is no need for PCR or cloning, as digestion of the destination vector with SapI...

... the packaging cell genome, bacterial genomes, cloning vectors, and helper plasmids (Chadeuf et al., 2005...

...Fluorescent Proteins (FP) available for microscopy, by cloning your protein of interest into a vector encoding...

.... Cas9 guide RNA delivery does however rely on cloning into a vector or in vitro synthesis, which could...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

... are typically knocked-in via gene targeting or cloned in-frame to the protein of interest for plasmid-based...

...use in western blots, though the original parent clone (M2) has been widely used in a variety of applications...

...experiment today! Get ready to identify knock-in clones by reading up on validating genome edits to find...

... size, more durable, and usually much easier to clone, express, and purify compared to antibodies. Nanobodies...

...method. The general steps for this method include: Cloning and transforming a plasmid into the bacterium Agrobacterium...

... Congenital Amaurosis (LCA). Shortly after the cloning of the RPE65 gene, a naturally occurring animal...

...yeast strain that also contains a reporter gene cloned downstream of a promoter containing the target ...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

... years of focusing on my technical skills with cloning and lentivirus production, I didn’t really start...

...protein fusion. This one protein method simplifies cloning and makes your experimental setup less cumbersome...

...control elements for each would be relatively large. Cloning the viral genes in this plasmid would be more difficult...

...protocols and guides regarding pooled libraries, from cloning a pooled library to viral production to screening...

... If you don’t feel like doubling the amount of cloning, another option is to use variants of Cas9, like...