962 results

Learn about some ways to study behavioral neuroscience with viral vectors using behavioral tests for locomotion, anxiety, and depression.

Sept. 17, 2019, 12:40 p.m.

Learn about new and upcoming viral vectors from Addgene including our new trial size AAVs for serotype testing, calcium and GABA sensors, and more!

Sept. 12, 2019, 1 p.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

Stop waiting for your MTA approval to go through & talk to your technology transfer office. Find tips for working with your university TTO as a researcher.

Sept. 5, 2019, 1:06 p.m.

Learn about new plasmids at Addgene including CRISPR, auxin inducible degradation, MerMAID channelrhodopsins, and genome looping.

Sept. 3, 2019, 1:15 p.m.

Are you going to present or attend a scientific conference soon? Find tips for success as a presenter and attendee.

Aug. 29, 2019, 12:50 p.m.

Are you looking to make your office more eco-friendly? Read this blog post to learn what we've been doing to make our office more sustainable!

Aug. 27, 2019, 1:08 p.m.

Designing a cloning experiment? Read this blog post to learn about different positive and negative selection strategies you can take to find the clones you're looking for.

Aug. 22, 2019, 12:43 p.m.

If you're new to behavioral neuroscience, this article will walk you through how to standardize your experiments and chose viral vectors appropriate for your experiment.

Aug. 20, 2019, 12:31 p.m.

Joining the popular sacB counterselection method is a new suite of toxin-based allelic exchange vector that can be used in many proteobacterial genomes.

Aug. 15, 2019, 12:30 p.m.

Zebrafish larvae is a great model for studying development and embryogenesis. Learn more about genetic tools for zebrafish in this blog post.

Aug. 13, 2019, 12:58 p.m.

New to peer-review? This peer-review checklist takes you through different steps and things you should consider while writing a peer-review. Remember, be critical and kind and advocate for open science.

Aug. 8, 2019, 1:19 p.m.

Getting start with mouse models? Here, we break down how to cross mice to create double knockout lines and Cre-lox lines.

Aug. 6, 2019, 12:55 p.m.

There are many ways to study Dopamine including in vivo microdialysis and genetically encoded biosensors. Read on to learn about two recently developed biosensors: dLight and GRABDA.

July 30, 2019, 12:59 p.m.

Transient plant leaf transformation can save you time... months in fact. Learn more about this method and get some tips and tricks for designing your transient plant leaf transformation experiment.

July 25, 2019, 12:03 p.m.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

July 23, 2019, 12:46 p.m.

Learn about the different open resources for C. elegans like OpenWorm, microPublication, and 3'UTRome, and find C. elegans tools at Addgene.

July 18, 2019, 12:55 p.m.

Zebrafish make a great model organism for studying neuroscience. Here, we describe how optogenetics can be used to study zebrafish behavior like swimming.

July 16, 2019, 12:59 p.m.

Learn about the common techniques to creating engineered mouse models at embryonic stages and the pros and cons of different genome editing techniques.

July 11, 2019, 1:26 p.m.

Learn ways you can bring your science to elementary and middle school by hosting events and giving talks geared towards elementary and middle school students.

July 9, 2019, 12:52 p.m.

Learn about CRISPR activation tools for neuroscience including a SPH transgenic mice, neuron-optimized viral vectors, and high-throughput screening.

July 2, 2019, 12:51 p.m.

There are many tools available for studying the microbiome. Learn about plasmid tools for engineering gut microbes, detection, and synthetic biology.

June 27, 2019, 12:25 p.m.

Learn about the different ways you can introduce DNA or RNA into cells using methods such as transformation, transduction, conjugation, and transfection.

June 25, 2019, 12:54 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including our new trial size AAVs for serotype testing, jGCaMP7c variant in AAV1, Flp-expression AAV, and more.

June 18, 2019, 1:15 p.m.

Addgene has many videos to walk you through lab protocols. Find videos about AAV purification, AAV titration, and the basics working with tissue culture.

June 13, 2019, 12:19 p.m.

Learn about free tools for making scientific graphics: BioRender, Vectr, Inkscape, Google Drawing, Smart Servier Medical Art, and Google Image Search.

June 11, 2019, 1:16 p.m.

Learn about new plasmids including optogenetic control of synaptic transmission, acoustic reporter genes, miRNA reporter vectors, and CRISPR-Cas9 delivery for in vivo screening.

June 6, 2019, 12:41 p.m.

Learn about the fluorescent protein work being supported by the Michael Davidson and Rogers Tsien Commemorative Travel Awards.

June 4, 2019, 1:02 p.m.

Read on to learn more about this technique to isolate specific genomic regions using CRISPR.

May 30, 2019, 12:44 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

May 28, 2019, 12:49 p.m.

What you get out of a conference depends on what you put into it before, during, and after the meeting. Read on to find tips for honing your elevator pitch, the elevator pitch, navigating talks, networking, and social media.

May 23, 2019, 12:26 p.m.

FlipGFP is a genetically encoded caspase reporter that contains a flipped beta strand of GFP. There is no fluorescence unless the caspase cuts the cleavage site between the strands.

May 21, 2019, 12:10 p.m.

Addgene is hosting #AddgeneCRISPRweek on Twitter. Check it out for anything from CRISPR basics to new CRISPR tools.

May 13, 2019, 12:01 p.m.

CellTag is a lentiviral based tagging strategy that combines scRNA-seq and lineage tracking at the single-cell level.

May 9, 2019, 1:15 p.m.

CTD2 FASMIC is collection of plasmids that can help scientists functionally characterize thousands of mutations associated with cancer.

May 7, 2019, 12:39 p.m.

Learn more about unique organisms that are being developed as model systems in this blog post.

May 2, 2019, 12:41 p.m.

mNeonGreen is a bright green fluorescent protein. Along with mWasabi and mTFP, it can be used for traditional imaging, FRET, and multicolor imaging.

April 25, 2019, 3:01 p.m.

Create a more sustainable lab by making small changes in your day-to-day or aim for long-term conservation goals. Find easy action items in the blog post.

April 22, 2019, 12:26 p.m.

CRISPR-Sirius is a new tool to visualize genomic loci using dCas9. It's brighter than its predecessor CRISPRainbow and unlike fluorescent in situ hybridization, can be used in live cells.

April 18, 2019, 12:53 p.m.

The Addictive Brain is a new platform to communicate science to broad audiences. Check out this blog post to learn more and for some science communication tips.

April 16, 2019, 1:05 p.m.