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Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

Course based undergraduate research classes use lab time to dive into real-world experiments. This guest post introduces CURE concepts and application.

Feb. 9, 2023, 2:15 p.m.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

Aug. 27, 2020, 1:15 p.m.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

July 31, 2020, 12:30 p.m.

Sarting a journal club? Read our guide to the logistics of forming a journal club and find different formats to hold the journal club discussion.

Feb. 6, 2020, 3 p.m.

Find tips and tricks for using digital droplet PCR to quantify your AAV titers.

Jan. 16, 2020, 2:18 p.m.

Find plasmids more easily with the new search feature on Addgene's website! Now, you can set filters to narrow down your plasmid or viral service search.

Dec. 3, 2019, 11:38 a.m.

Crossing the blood brain barrier is difficult for many AAVs. But, PHP.B has impressive CNS transduction in some mice. Learn more about how PHP.B and about its strain specific performance.

July 23, 2019, 12:46 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Cas13a is an RNA cleaving enzyme that can be used to detect specific RNAs. Learn about this and other applications, like nucleic acid detection.

Sept. 21, 2017, 2:07 p.m.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

May 19, 2016, 2:30 p.m.

This blog posts details what it's like to be a grant writing professional and how to get into the grant writing profession.

Aug. 20, 2015, 2:30 p.m.

Developments in light sheet fluorescence microscopy (LSFM) and tissue clearing enable researchers to visualize tissues of transgenic and non-transgenic organisms in 3D.

July 16, 2015, 2:30 p.m.

Protein tags can be used for purification, detection, localization, and more. Learn more about the molecular tool box of tags and fusion proteins.

Dec. 11, 2014, 4:26 p.m.

An all-in-one CRISPR/Cas9 Multiplex Genome Engineering System for transfecting and editing cell lines.

Nov. 12, 2014, 3:54 p.m.

Addgene's viral vector service has grown much since it was first launched in 2016! Read about our service and the unique challenges it presents in this blog post.

July 30, 2024, 1:15 p.m.

With many different Cas9 variants out there, it can be hard to know which one to use in your particular CRISPR experiment. This post is here to help.

Oct. 13, 2022, 1:45 p.m.

Zebrafish larvae is a great model for studying development and embryogenesis. Learn more about genetic tools for zebrafish in this blog post.

Aug. 13, 2019, 12:58 p.m.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Jan. 29, 2019, 2:27 p.m.

Plasmid addiction systems, used by bacteria to maintain plasmids in dividing cells, have been developed by scientists as molecular biology tools.

Nov. 1, 2018, 12:35 p.m.