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TypeBlog PostPublishedJuly 12, 2016, 2:30 p.m....as effective at editing mouse embryos as Cas9 from S. pyogenes and has the advantage of being smaller (...health. References Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini...
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Easing the Protein Purification Process with pCri
TypeBlog PostPublishedJune 19, 2015, 3:08 p.m..... Young, Carissa L., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification... -
The Blue Flame Award: Celebrating Addgene's Most Requested Depositors
TypeBlog PostPublishedNov. 14, 2016, 3:30 p.m...., Addgene has “literally enabled sharing with 100's of investigators worldwide. Now everything from every... -
Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes
TypeBlog PostPublishedJune 19, 2018, 1:38 p.m.... al also used arg1 to in vivo image tumour-homing S. typhimurium strain ELH1301. Bourdaeu et al see this... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m....photoconvertible proteins. With Sattarzadeh et al.’s discovery that GFP itself is photoconvertible, new... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....efficiency of BAC/PAC recombineering techniques. Semprini S, Troup TJ, Kotelevtseva N, King K, Davis JR, Mullins... -
CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]
TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....1):81-89. PubMed. 2. Bauer DE, Kamran SC, Lessard S, Xu J, Fujiwara Y, Lin C, et al. An erythroid enhancer... -
The Stingy Scientist: How the Baby Gel Box Was Born
TypeBlog PostPublishedApril 15, 2014, 3:11 p.m....need them. When I was in graduate school in the 80’s, my lab was well funded and we didn’t feel the need... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....PubMed PMID: 24727401. Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural... PubMed Central PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted... -
Antibodies 101: Fc Effector Functions
TypeBlog PostPublishedMay 18, 2023, 1:15 p.m....10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan, N. S., & Casadevall, A. (2016). Ig Constant Region Effects... -
CRISPR 101: Drug discovery and CRISPR
TypeBlog PostPublishedMay 4, 2023, 1 p.m....parental cell line and the X knockout with your drug(s). If a drug is equally toxic to both lines, it probably... -
Plasmids 101: Gene, enhancer, and promoter trapping
TypeBlog PostPublishedMay 2, 2023, 1 p.m....DOI: https://doi.org/10.7554/eLife.67667 Springer, S. P. Gene Traps. Plant Cell. (2000) 12(7): 1007-1020... -
Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?
TypeBlog PostPublishedJune 9, 2016, 2:30 p.m....lower potential for off-target effects. In Gao et al.’s experiments, a single mismatch at any position of ... -
Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
TypeBlog PostPublishedJuly 18, 2017, 12:41 p.m....134:1-17. PubMed PMID: 27984002. 2. Bilan, Dmitry S. et al. “HyPer-3: A Genetically Encoded H2O2 Probe... -
New and Upcoming Viral Vectors - Spring 2019
TypeBlog PostPublishedMarch 26, 2019, 1:40 p.m.... Plasmid Serotype Name 50363 AAV5 AAV phSyn1(S)-DreO-bGHpA 121675 AAV9 pAAV-EF1a-fDIO-Cre (FLP-dependent... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....in the number of steps in the protocol. IgnaviCas9’s incredible thermostability makes the enzyme particularly... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m....after infection with S. aureus (red). You can then see neutrophils swarming around S. aureus and eating ... -
Antibodies 101: Designing Your First Flow Panel
TypeBlog PostPublishedMay 28, 2024, 1:45 p.m....global excitation maximum lies around 650 nm, APC-Cy7's global excitation maximum is about 750 nm. If we were...fluorophores show efficient absorption at 650 nm, APC-Cy7's optimal excitation happens at higher wavelengths of... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog PostPublishedAug. 2, 2022, 1:15 p.m....resources References Silhavy, TJ., Kahne, D. & Walker S. The Bacterial Cell Envelope. Cold Spring Harb Perspect... -
Hot Plasmids and Viral Preps - September 2021
TypeBlog PostPublishedSept. 21, 2021, 1:15 p.m....DNA repair. A new CRISPR pooled library targeting S. pneumoniae D39V from Jan-Willem Veening is now available...