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We narrowed to 628 results for: Dos

Showing: 221 - 240 of 628 results

  1. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    Published
    Aug. 30, 2022, 1:15 p.m.
    ... on the inside of the cell. However, assuming you do need to permeabilize, you have a few options for ...organellar membranes. Created with BioRender.   How do I choose?! Ok, so now you know some of the possible...possible types of fixatives or permeabilizers, but how do you decide which ones to use? Here are some tips ...

  2. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...Accuracy needed Do you need to call exact copy numbers or just general trends? How confident do you need to...researchers can use to quantify said copies. They do so using copy number variation (CNV) assays. There...

  3. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    Published
    June 24, 2025, 1:15 p.m.
    ...deposit had already moved on to their next position. We do routinely review deposits that are left in the hold...materials the way we will with journal articles. But we do encourage you to consider sharing them openly as ...

  4. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...disease. Figure 1: Scientists are continuing to do essential research into COVID-19 (like here at Addgene...boundaries. To be able to beat this virus, we will need to do the same with science,” said Guillaume Bourque, a...

  5. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...questions about scientists on Twitter: who are they? What do they share? And how they are connected? Here are ... (left), life (center), and physical (right). How do you use Twitter as a scientist? If you don’t use ...

  7. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...shadowing members of my team to learn what I was to do for my time at Addgene. This training prepared me...where each team gives a presentation on what they do and how it relates to other teams at the company....

  8. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...you may not have the chance to stop what you’re doing and make a phone call or write out an email. You...which you can describe your issue and press send. Doing so will bring your concern directly to our support...

  9. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...education? Let us know in the comments section below! Do you know of a society or event which would benefit...While we are not able to help with every request, we do aim to support events and scientific community activities...

  12. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...and institutions are not aware of what Addgene can do for them and how the repository can help them share...other side of the force and as an Addgenie I will do all my best to help them in their tasks. But I won...

  13. Advice for Choosing a Research Project

    Type
    Blog Post
    Published
    April 17, 2014, 2:45 p.m.
    ...experiments and why? What techniques and skills do you need to use to reach these milestones? Can you...milestones along the way so that you don't get stuck doing the same experiment for too long. Keep evaluating...

  14. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ... yourself with this passion. “Love what you are doing!” It helps when the time comes to work with full...become more aware of careers outside of academia. How do PIs assist their mentees who have goals outside of...

  15. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type
    Blog Post
    Published
    July 1, 2025, 1:15 p.m.
    ...move on to your downstream application — but wait! Do you know how much plasmid DNA you have or how pure...absorbance readings are inherently more variable. If you do need to work with dilute samples:  Take multiple...the references we have provided below. And if you do have trouble with contamination, feel free to check...

  16. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... team at Calico Life Sciences recently set out to do just that — by developing a new fluorescent protein...fluorescent proteins display a similar effect (many do). Fusing EGFP to the self-labeling FlavinTag made... MagLOV raise broader questions like “How and why do fluorescent proteins respond to magnetic fields?”...

  17. SciComm: Taking Science to Elementary and Middle Schools

    Type
    Blog Post
    Published
    July 9, 2019, 12:52 p.m.
    ... the school district itself in terms of what they do with their discretionary money. They also usually...for any topic--not just CRISPR): Who am I? Where do I work? The teachers emphasized that some students...and other science communicators.  What can CRISPR do? I spent more time on this section of the presentation...

  18. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    Published
    Feb. 27, 2018, 2:04 p.m.
    ...Western blots. ELISAs. Immunofluorescence. What do all of these techniques have in common? They all typically...staining Can be expensive Nanobodies   Recombinant; do not require animal sacrifice Good tissue penetration...Unlike large secondary antibodies, small nanobodies do not oligomerize with primary antibodies when incubated...

  19. Transferable Skills Guide: Identifying Your Transferable Skills

    Type
    Blog Post
    Published
    Jan. 11, 2018, 3:29 p.m.
    ... out of the park. It’s clear that you know how to do the job. You may think this means you’re guaranteed...to thrive at the company and add value, not just “do the job”. When they ask about your creativity, your...good but focused on all the other things you have to do. Pause! Enjoy that feedback, bask in the glow of ...

  20. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...CRISPR could cut DNA, the second generation could do base editing, and with the third generation came ...? The AbuGoot lab realized that they could likely do it using Prime editing alongside some creative (and...represent a major step forward for what the field can do.    References and Resources References  Özcan A...integrases.   Use PASTE can be delivered via a single dose of plasmids, works in non-dividing and primary cells...
Showing: 221 - 240 of 628 results