We narrowed to 228 results for: cloning

1. Hot Plasmids: Spring 2025

   Type

   Blog Post

   Published

   June 3, 2025, 1:15 p.m.

   ...backbone plasmids here! Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079...

2. Antibodies 101: Flow Cytometry

   Type

   Blog Post

   Published

   July 20, 2021, 1:15 p.m.

   ... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution...

3. Cancer and the Immune System: Deciphering the Relationship

   Type

   Blog Post

   Published

   March 14, 2017, 2:30 p.m.

   ...Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ...

4. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

   Type

   Blog Post

   Published

   June 28, 2018, 3:01 p.m.

   ... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message...

5. Deep Dive: qPCR

   Type

   Blog Post

   Published

   Sept. 29, 2022, 1:15 p.m.

   ...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

6. Hot Plasmids - May 2022

   Type

   Blog Post

   Published

   May 26, 2022, 1:15 p.m.

   ... own subclone, they were able to develop a 12-plex multiplex imaging system. Mixing the subclones within...their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry, localization peptides...

7. Tips for Titering Your Lentiviral Preps

   Type

   Blog Post

   Published

   March 15, 2016, 2:30 p.m.

   ...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

8. Antibodies 101: Introduction to Antibodies

   Type

   Blog Post

   Published

   Jan. 19, 2021, 2:15 p.m.

   ...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...