We narrowed to 237 results for: cloning

...yeast strain that also contains a reporter gene cloned downstream of a promoter containing the target ...

...transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer would be particularly...

... gene for bacterial selection. The process for cloning the 20-nt guide RNA is identical for both vectors...

...virus, I learned from Addgene,” or “I learned to clone from the Addgene website.” We delight in seeing ...

...SELEX). After 4-6 rounds of SELEX, the RNA pool was cloned into bacterial expression vectors to create a library...

... or q, respectively). These sequences were then cloned into an AAV backbone containing a minimal promoter...

...McIntyre, G. J., & Fanning, G. C. (2006). Design and cloning strategies for constructing shRNA expression vectors...

...backbone plasmids here! Figure 2: Addgene cloned EGFP into pAG Lenti CMV N-HA Puro (Addgene #236079...

...mouse embryonic stem cells. After selecting for clones with the recombined product, you’d microinject ...

... you spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution...

...self-labeling protein fusions as well as empty backbones to clone your own protein of interest. Fluorescence microscopy...

...Cancer Drug Resistance Checkout the RAS Pathway Clone Collection ...

... alleles may be warranted (20-30% of all tagged clones were bi-allelic in our hands). Take-home message...

...Digital Droplet PCR for AAV QuantificationPlasmid Cloning by PCR...

... own subclone, they were able to develop a 12-plex multiplex imaging system. Mixing the subclones within...their work with the “signalome,” a 12-plex mixed-subclone system using mStrawberry, localization peptides...

...sorting based on expression of a surface marker, subcloning, expansion, and confirmation. This procedure ...

...antigen to myeloma cells. The hybridomas are then subcloned by limiting dilution, which produces an immortalized...