We narrowed to 706 results for: plasmid

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

Lentiviruses are useful for delivering DNA sequences to non-dividing mammalian cells. Learn all about the components of lentiviral vectors inside.

May 19, 2016, 2:30 p.m.

Viral minigenomes provide a safe way to study dangerous viruses like Ebola. Read all about minigenomes inside.

March 17, 2016, 2:30 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for base editing, controlling gene expression in yeast and Caulobacter crescentus, multiplexed gRNA for human pluripotent stem cells, CRISPR-based DNA demethylation.

March 10, 2020, 1:15 p.m.

The Michael Davidson lab deposited their collection of 3,300 fluorescent protein plasmids including empty backbones to Addgene.

July 29, 2014, 6:16 p.m.

Learn about CRISPR plasmids at Addgene such as an E. coli genome-wide CRISPRi library, GeneWeld vectors, CasX plasmids, and drug inducible CRISPR/Cas9 activation.

Sept. 10, 2019, 12:56 p.m.

Twenty years have gone by fast! Join us to share some highlights of Addgene and our community as we close out our 20th anniversary celebration.

Dec. 19, 2024, 2:15 p.m.

With this NIH's BRAIN Initiative Grant, Addgene will provide ready-to-use recombinant antibodies and will build a crowdsourced antibody Data Hub.

May 25, 2021, 2:29 p.m.

Learn about the many ways you can deliver reprogramming factors to generate induced pluripotent stem cells (IPSCs).

April 17, 2018, 1:37 p.m.

SapTrap improves CRISPR genomic tagging in C. elegans by offering scientists an easy one tube assembly reaction to generate high-throughput targeting vectors.

Oct. 12, 2017, 2:26 p.m.

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

Aug. 4, 2020, 1:15 p.m.

Addgenie Eric Perkins curates Addgene resources you can use in your chromatin research.

June 12, 2018, 11:40 a.m.

Learn about the differences in virus production between insect cells and mammalian cells.

Nov. 5, 2024, 8:32 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Antibiotic resistant bacteria are a huge issue in the clinic - learn how CRISPR is being developed to fight against antibiotic resistant super bugs.

May 3, 2016, 2:30 p.m.

Addgene's conference rooms are named after gene names. Learn more about these genes including sonic hedgehog, sleeping beauty, groucho, and cookie monster.

April 2, 2019, 1:44 p.m.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Sept. 29, 2015, 2:30 p.m.

Addgene has reached 100,000 total reagents in the repository! The next milestone coming up is 100,000 and we'd like you to guess the date that happens (a contest).

April 8, 2021, 7:25 p.m.

Interview with Professors Eric Mendenhall and Richard Myers about their CRISPR- based system for tagging any endogenous gene of interest.

July 28, 2015, 2:30 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene including plasmids for CRISPRi in Burkholderi, DNA and RNA editing, epitope tagging, and Cas9/gRNA ribonucleoprotein delivery.

Dec. 17, 2019, 2:37 p.m.