We narrowed to 293 results for: promoter
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TypeBlog PostPublishedApril 14, 2016, 2:30 p.m....was shown that putting the operon under a strong promoter results in high R-body expression (Schrallhammer...
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CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....binding proteins with Cas9 guided by different promoters. The challenge in building genetic circuits is... -
Prime Editing: Adding Precision and Flexibility to CRISPR Editing
TypeBlog PostPublishedJan. 13, 2025, 9:49 p.m....complex, to temporarily inhibit this process and promote copying of the edited strand to the unedited strand... co-express mismatch repair inhibitor MLH1dn Promote desired resolution of heteroduplex ✓ ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....inhibitor response 1 (TIR1) expressed under a desired promoter with a specific spatiotemporal expression pattern... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....its paralog RC2H2 are RNA binding proteins that promote RNA decay and their upregulation is correlated ... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....under the control of Murine Stem Cell retrovirus promoter for mammalian gene knockdown. Vector provided ... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....several weeks which may also depend on transgene or promoter choice. Behaviors you can study using viral vectors... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....nuclear localization, binding to the appropriate promoter sequence and activation of gene expression. In... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics
TypeBlog PostPublishedNov. 3, 2020, 2:15 p.m....dopamine is an important neurotransmitter that promotes reward-motivated behavior, if the channelrhodopsins... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m.... had multiple integration events. In addition, promoter choice can also impact transgene expression. The... -
The Future of Research Symposium Boston 2015
TypeBlog PostPublishedOct. 20, 2015, 2:30 p.m....Program at Harvard Law School. He has continually promoted the cause of increasing data collection about ... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....orientation. This allows for multiple DNA components (promoters, genes, terminators, etc) to be assembled in the... -
A Look at Addgene's QC Process
TypeBlog PostPublishedMay 6, 2025, 1:15 p.m....they ordered contains the correct inserts, tags, promoters, and other critical functional components. Fun... -
Progress Towards a PAM-Free CRISPR
TypeBlog PostPublishedMarch 7, 2024, 2:39 p.m....surprise since the PAM flexibility could in theory promote off-target binding. The activity of SpRY was validated... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog PostPublishedDec. 14, 2021, 2:15 p.m....functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....papers. Twitter Scientists often use Twitter to promote their work, so it’s also a good place to look for... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....discussions. Get your voice out there. Use Twitter to promote your other science communication activities, e.... -
Overcoming the Challenges of Lentiviral Production
TypeBlog PostPublishedAug. 7, 2018, 12:31 p.m....incorporating it into a fourth plasmid. In addition, the promoter of the 5’ long terminal repeat (LTR) is mutated... -
dTAG - You're it!
TypeBlog PostPublishedJune 21, 2018, 2:06 p.m.... al. YY1 Is a Structural Regulator of Enhancer-Promoter Loops. Cell. December 14 2017. 171(7):1573-1588...