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Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting human and mouse genes.

Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.

Get started with Gibson Assembly Cloning!

Protocol that can be used to titer lentiviral preparations carrying a fluorescence marker.

Use this protocol to transfect mammalian cells with your plasmid of interest.

Use this protocol to generate lentivirus

This protocol can be used to titer lentiviral preparations that confer antibiotic resistance.

Information about plasmid cloning by restriction enzyme digest (subcloning), including design and experimental procedures.

Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector.