We narrowed to 296 results for: promoter

Learn how repressible promoters work and how you can use them in your next experiment.

Dec. 20, 2022, 2:15 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

An introduction to gene, enhancer, and promoter trapping. Relevant examples of each along with pros and cons of each system.

May 2, 2023, 1 p.m.

A toolkit of synthetic promoters for transgene expression in AAV can be used for targeted gene expression in retinal cell types in mice, primate, and human retinas.

March 12, 2020, 1:15 p.m.

Some promoters are only active under specific circumstances. Here's a guide to inducible promoters, which are regulated by chemicals, light, and/or temperature.

Jan. 18, 2018, 2:34 p.m.

Learn all about CAPTURE - a new CRISPR-based technique to study regulatory elements in their native chromatin contexts.

July 2, 2020, 12:40 p.m.

Gene expression is often imprecise due to variations in gene copy number within the cell (either on plasmid or genome). New promoters from Chris Voigt’s lab enable stable gene expression regardless of copy number regardless of growth phase and whether they are expressed on the plasmid or chromosome.

Sept. 4, 2018, 12:53 p.m.

Learn about pSiM24, a new binary vector drives gene expression in plants.

Sept. 29, 2015, 2:30 p.m.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.

June 17, 2021, 1:17 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Learn about the process to select the optimal serotype, promoter, and titer for your AAV experiment.

June 1, 2023, 1:15 p.m.

A review of protein expression mechanisms for on, off, and tunable, control of protein expression.

Feb. 16, 2023, 7:05 p.m.

Learn about SF-iGluSnFr, an updated glutamate biosensor from Loren Looger's lab.

April 7, 2022, 1:15 p.m.

Choose the best empty backbone for your experiment from some of the most popular in Addgene's library.

Aug. 19, 2014, 3:39 p.m.

Learn about different strategies used to generate plasmids for multiplex gRNA expression and how to use them in various organisms.

Sept. 10, 2020, 11:45 a.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

In this blog post, we explain gamma-retrovirus packaging systems for those interested in using gamma-retroviral systems in the lab.

Aug. 6, 2024, 1:15 p.m.

Announcing Addgene's AAV Data Hub! The Data Hub is an open platform for scientists using AAV to share practical information about their experiences.

Oct. 8, 2019, 12:33 p.m.

Learn about the various expression systems and plasmids you can use to produce your protein of interest.

June 7, 2018, 1:17 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.