962 results

Model organisms are species that are widely studied because they are easy to maintain and modify in the lab. Common model organisms include the mouse, the fruit fly, yeast, zebrafish, and the worm.

April 11, 2019, 12:39 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Addgene's conference rooms are named after gene names. Learn more about these genes including sonic hedgehog, sleeping beauty, groucho, and cookie monster.

April 2, 2019, 1:44 p.m.

Learn about recently available viral vectors and viral vectors coming soon from Addgene's viral service including calcium sensors, GABA sensors, voltage sensors, and control AAV.

March 26, 2019, 1:40 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene. Read about CRISPR-Cas12b for human genome editing, plant base editors, string assembly gRNA cloning, lineage tracing tools, and more.

March 21, 2019, 12:47 p.m.

Learn all about new plasmids available at Addgene including an anti-CRISPR optogenetic tool, the fluorescent voltage indicator Archon1, photoswitchable proteins, and a simplified 2in1 cloning method.

March 19, 2019, 1:31 p.m.

Exosome-AAVSs, found in small membrane vesicles that are secreted from cells, can be purified and have some advantages over traditionally prepared AAVs.

March 14, 2019, 1:54 p.m.

The academic job interview chalk talk is essentially a million dollar sales pitch about your research vision. Learn how to define your research vision, organize your chalk talk, and practice efficiently before your next interview!

March 12, 2019, 1:37 p.m.

Find ideas to make your research more environmentally friendly. In this blog post, learn how to you can evaluate products to choose more sustainable ones, reuse plastic, and repurpose regulated medical waste.

March 7, 2019, 2:39 p.m.

Stochastic multicolor labeling is a popular technique to label individual cells with different colors and allows for lineage tracing of cell populations over time. Read this blog post to learn about the different systems for various organisms.

March 5, 2019, 1:08 p.m.

Learn about the Reproducibility for Everyone collaboration and how you can bring reproducibility workshops to your conference or university.

Feb. 28, 2019, 1:34 p.m.

Download Addgene's Science Career Guide and find some career tips from Addgenies.

Feb. 26, 2019, 1:40 p.m.

Targeted mutagenesis helps molecular biologists make changes to a DNA sequence at a precise location. Read on to learn more about EvolvR, a CRISPR-Cas9 based targeted mutagenesis method from the Dueber Lab.

Feb. 21, 2019, 1:15 p.m.

Addgene welcomes Karen Katz, Esq and Carl Paratore, MBA, CPA to their Board of Directors. Meet them and learn more in this Q&A.

Feb. 19, 2019, 1:36 p.m.

Thinking about ways to make your lab more environmentally friendly? In the first installment of Bringing Sustainable Practices to the Lab, learn about how to recycle styrofoam boxes, plastic packaging, and gloves.

Feb. 14, 2019, 2:35 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

Leaving the lab bench for a career outside of academic research can be a daunting task! In this blog post, we describe some ways to ease the transition from bench science to the office.

Feb. 5, 2019, 1:04 p.m.

The tumor microenvironment is the cellular milieu that surrounds a tumor and contains immune cells, blood vessels, cells, and signaling molecules. What goes on in the cancer microenvironment affects and is affected and can either promote or repress tumor growth.

Jan. 31, 2019, 1:32 p.m.

Aptamers are nucleic acid oligos that bind to small molecules to induce their fluorescence and can be used to study RNA localization and dynamics.

Jan. 29, 2019, 2:27 p.m.

Stochastic multicolor labeling is a powerful tool for discriminating between neurons. Learn about how a new system named Tetbow (Tetracycline transactivator Brainbow) can bring brighter multicolor labeling to neuronal tracing experiments.

Jan. 24, 2019, 2:24 p.m.

Fluorescent biosensors can be used to measuring changes in autophagic activity over time. Several autophagy biosensors, such as Rosella, Keima, and tandem fluorescent proteins are described.

Jan. 22, 2019, 2:41 p.m.

Electronic lab notebooks (ELNs) are useful tools that help researchers keep track of data. However, not all ELNs support the same functionality when it comes to reproducibility.

Jan. 17, 2019, 1:38 p.m.

The results from our #DeckTheLab social media contest are in! Read this blog post to see the Addgenie's favorite lab decorations.

Jan. 15, 2019, 2:19 p.m.

Addgene celebrates 15 years of scientific sharing. In this post, executive director Joanne Kamens reflects on the past and future of Addgene.

Jan. 14, 2019, 2:08 p.m.

Phage Directory is a directory of phage researchers and the phages they have in their labs with the goal of helping clinicians and scientists quickly get phages for phage therapy. Now, Phage Directory is also expanding to provide educational tools and resources to the phage community.

Jan. 10, 2019, 2:38 p.m.

Addgene reached its 15 year anniversary on January 8, 2019. In this blog post, find the top 15 requested plasmids since our founding. Many of these include lentiviral or CRISPR tools!

Jan. 8, 2019, 1:54 p.m.

Learn about CRISPR plant base editors for plants

Jan. 3, 2019, 1:35 p.m.

The CRISPR babies saga shows us that we need action to move forward in bringing CRISPR technologies from bench to bedside.

Dec. 20, 2018, 1:15 p.m.

This blog series showcases some of the new CRISPR tools we have at Addgene each quarter.

Dec. 18, 2018, 1:17 p.m.

The Addgene viral team has found a way to increase AAV yields through media supplementation of sucrose, an endocytosis inhibitor.

Dec. 13, 2018, 1:14 p.m.

If you're a scientist, you should use social media for networking and professional development! Read this blog post for some reasons why and five resources that can help you get started.

Dec. 11, 2018, 12:46 p.m.

Learn all about how to use a new plasmid for RNAi in C. elegans, tools for proximity labeling, RNA-based methods for detecting viral outbreaks, and tools for characterizing protein domain binding specificities.

Dec. 6, 2018, 1:34 p.m.

Cas14 was discovered by the Doudna lab. It cleaves ssDNA (but not dsDNA) in trans and can be adapted for a diagnostic toolkit such as DETECTR.

Nov. 29, 2018, 1:53 p.m.

Seeding Labs and Addgene collaborate to provide scientific resources, equipment, and plasmids to researchers in resource-limited settings. Read more to learn about the recipients of the Addgene-Seeding Labs plasmid grant this year.

Nov. 27, 2018, 2 p.m.

The purpose of this article is to explain what Unique Persistent Identifiers are, why they’re important, and to announce that Addgene now supports two different formats of plasmid identifiers and how they should be used.

Nov. 20, 2018, 8:17 p.m.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Nov. 15, 2018, 1:50 p.m.

If you're a grad student looking to do a postdoc, you may be considering an academic or an industry postdoc. This article describes the pros and cons of both settings for your postdoc experience.

Nov. 13, 2018, 1:22 p.m.

Scientists are now using Addgene's plasmid repository as a source of sequence data sets. Researchers from MIT built a classifier that predicts whether a gene sequence is natural or synthetic and found that more and more genes are being sourced from farther away in the tree of life.

Nov. 8, 2018, 3:47 p.m.

Learn how stem cell researchers use reprogramming and transdifferentiation techniques to convert cells from one type into another.

Nov. 6, 2018, 1:12 p.m.