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Plasmids 101: Methylation and Restriction Enzymes

Type

Blog Post

Published

June 30, 2016, 2:30 p.m.

...sequence of the DNA surrounding your restriction site(s) can help you quickly predict which (if any) of those...
Screening for Successful Genome Editing with Digital PCR

Type

Blog Post

Published

March 30, 2017, 2:30 p.m.

...24509632. PubMed Central PMCID: PMC4063274. 2. Findlay, S. D., Vincent, K. M., Berman, J. R. & Postovit, L.-...
Why and how to start studying chromatin (with Addgene's help)

Type

Blog Post

Published

June 12, 2018, 11:40 a.m.

...reagents. Yamagata M, Sanes JR Proc Natl Acad Sci U S A. 2018 Feb 13. pii: 1722491115. Addgene blog posts...
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Type

Blog Post

Published

June 6, 2019, 12:41 p.m.

...reporter podcast segment Discovered in the early 1990’s, microRNAs (miRNAs) are recognized as important regulators...
Early Career Researcher Toolbox: Social Media for Scientists

Type

Blog Post

Published

Dec. 11, 2018, 12:46 p.m.

... consistently engage with others about your topic(s) of interest. Hopefully through these links, I’ve:...
Hot Plasmids - February 2022

Type

Blog Post

Published

Feb. 22, 2022, 2:15 p.m.

...single expression cassette is advantageous to Cas9’s larger expression requirements (Figure 1), but Cas12a...
Viral Vectors 101: Gamma-Retroviral Packaging Systems

Type

Blog Post

Published

Aug. 6, 2024, 1:15 p.m.

...edition. References and resources References Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production ...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

Published

June 15, 2023, 1:15 p.m.

...come out! References Noren, C. J., Anthony-Cahill, S. J., Griffith, M. C., & Schultz, P. G. (1989). A General...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

Published

March 21, 2023, 1:15 p.m.

...PubMed Central PMCID: PMC49545. 3. Coffin J, Hughes S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory...
Sharing is Caring: But How to Distribute Open Hardware?

Type

Blog Post

Published

Feb. 28, 2023, 2:15 p.m.

...bioRxiv. 2020. Diederich, B., Lachmann, R., Carlstedt, S. et al. A versatile and customizable low-cost 3D-printed...
All in a Twist: dsRNA

Type

Blog Post

Published

Feb. 2, 2023, 2:15 p.m.

...1414 (2018). 10.1101/gr.237362.118 Chen, G. Y., Hur, S. Cellular origins of dsRNA, their recognition and ...
Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

Type

Blog Post

Published

Sept. 19, 2017, 1:20 p.m.

...Missinato, M. A., Onuoha, E., Perkins, L. A., Watkins, S. C., . . . Bruchez, M. P. (2016). A genetically targetable...
R Bodies: Membrane-Rupturing Microscopic Tools

Type

Blog Post

Published

April 14, 2016, 2:30 p.m.

...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

Published

June 2, 2015, 6:06 p.m.

...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Type

Blog Post

Published

April 22, 2015, 2:06 p.m.

...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

Published

July 25, 2017, 1:20 p.m.

...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Type

Blog Post

Published

Jan. 11, 2017, 3:30 p.m.

...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

Published

July 24, 2018, 12:51 p.m.

...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...
The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Type

Blog Post

Published

Jan. 31, 2019, 1:32 p.m.

...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...
Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

Type

Blog Post

Published

Nov. 3, 2020, 2:15 p.m.

...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

Showing: 241 - 260 of 345 results

Results per page:

Plasmids 101: Methylation and Restriction Enzymes

Screening for Successful Genome Editing with Digital PCR

Why and how to start studying chromatin (with Addgene's help)

Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

Early Career Researcher Toolbox: Social Media for Scientists

Hot Plasmids - February 2022

Viral Vectors 101: Gamma-Retroviral Packaging Systems

GCE4All: Making Genetic Code Expansion Accessible

Viral Vectors 101: Viruses as Biological Tools

Sharing is Caring: But How to Distribute Open Hardware?

All in a Twist: dsRNA

Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

R Bodies: Membrane-Rupturing Microscopic Tools

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

Lighting Up Cell Signaling with Photoswitchable Kinases

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Popular Retroviral Vectors and Their Uses in Scientific Research

The Breast Cancer Microenvironment: A Tumor’s Backstage Team

Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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