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We narrowed to 529 results for: arl

Showing: 241 - 260 of 529 results

  1. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ... glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by ...Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects ...

  2. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... under CC-BY 4.0 license.   Building off their earlier work on Rab11, the team used optogenetic clustering...constructs to the plasma membrane or lysosomes (the earlier article also provided CIB constructs for targeting...

  3. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ... interviews but no job offers, is a failure to clearly articulate one's talents and ideas. Some key points... matter how many times I say this, candidates regularly try to convince me that their search committee...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...other videos. For starters, I hadn’t anticipated nearly every webcam recording only 1280x720 resolution... to labs that need them. The lab re-opened very early in the pandemic with some incredibly strict safety...

  6. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...how much time you can possibly handle doing a particularly annoying task and then dedicating that much ...dinner plans at 6 pm, you had better get started early enough to finish everything you need to do by 6 ...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...into the parental plasmid (or other). This is particularly relevant in cases where a large plasmid with...introduce a point mutation in a large construct. Particularly, if a pair of unique restriction site is located...

  8. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    Published
    June 23, 2016, 2:30 p.m.
    ...using the same expression plasmid. This is a particularly important control when creating a phosphoserine-incorporation...Institute for Research in Madison, Wisconsin. She is particularly interested in the intersection of cell signaling...

  9. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...cars become more technologically advanced leaving earlier versions obsolete, the techniques we use in lab...researchers at a time when sequencing was still in the early days of development. Today, researchers use both...

  10. How to Negotiate a Successful Lab Start Up Budget

    Type
    Blog Post
    Published
    Sept. 1, 2015, 2:30 p.m.
    ...this way, if you know what you need. Most often, early career scientists aren’t trained to have these types...based on financial values. All too often, we see early investigators making unnecessary short term sacrifices...

  11. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...requires a nearby PAM, although these are still early days for this technology, and the user may need ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...

  12. CRISPR 101: Off-Target Effects

    Type
    Blog Post
    Published
    Feb. 8, 2024, 2:15 p.m.
    ...unanticipated sites. While off-target effects are nearly impossible to eliminate, it’s essential to understand...appropriate method for an activator or repressor Cas. Similarly, Cas ChIP-Seq would also identify all DNA binding...

  13. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ... R175H mutation to match a frequent mutation in early-onset GC and one with a mutation at R273H to match...Distinct TP53 Mutation Site-Linked Chemosensitivity in Early- and Late-Onset Gastric Cancer. Cancer Medicine,...

  14. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...different system (Issa et al., 2023). This can be particularly burdensome in the brain where many different...such as chicken-beta-actin or CAG can drive strong early gene expression, but can become methylated over ...

  15. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ...specific changes into the mouse genome. With these early technologies, a researcher would first introduce... is a molecular biologist with Biogen and is particularly interested in applying CRISPR for mouse genome...

  16. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ... does not mean that is all it will bind to - particularly when you start using complex samples such as... the antibody webpages. These outlets can be particularly useful for sharing negative data. Negative data...

  17. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...waivers. One of the blog posts that helped me quite early on was Christine Liu’s “How to Become a Scientist...on the Addgene blog Check out our resources for early career researchers Get advice for applying for jobs...

  18. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...to recognize DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition...engineering of novel base editor proteins. She is particularly interested in combining the realms of art and...

  19. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...bacteria or cell-free systems. The approach is particularly useful for producing drug precursors, biofuels...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...

  20. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... different colors. This lends flow cytometry particularly well to complex experiments looking at a variety...from the use of secondary antibodies. It’s also particularly useful for intracellular staining where binding...
Showing: 241 - 260 of 529 results