We narrowed to 706 results for: plasmid

Ever wondered how Addgene makes our viral preps? We explain it all here!

Oct. 24, 2023, 1:15 p.m.

Pooled libraries are a powerful tool, but they can difficult to prepare! Learn the tips and tricks for taking your pooled libraries from plasmids to screening.

July 13, 2023, 1:15 p.m.

Use blue light to control gene expression from PBAD promoters. It's more spatially and temporally precise compared to AraC based expression vectors.

June 17, 2021, 1:17 p.m.

Using synthetic biology principles has simplified and improved plasmids for optogenetic control of gene expression in E. coli.

Sept. 8, 2015, 2:30 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

History, composition, and tips for lentiviral vectors all in one convenient blog post.

March 21, 2017, 2:30 p.m.

Learn about the latest CRISPR plasmids deposited at Addgene such as an E. coli genome-wide CRISPR inhibition library, SECURE base editors, and Nme2Cas9.

May 28, 2019, 12:49 p.m.

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

Feb. 25, 2025, 2:15 p.m.

Overview of virus-specific safety controls as well as general safety procedures for viral handling.

June 22, 2023, 1:15 p.m.

In this blog post, learn how Addgene tracks samples and how you could implement a similar system in your lab.

Oct. 6, 2015, 2:30 p.m.

Learn how Addgene has worked to increase accessibility to our materials and educational resources.

Nov. 21, 2024, 10:47 p.m.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

June 18, 2020, 1:15 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Learn about what's coming for Addgene in 2026

Feb. 5, 2026, 4:15 p.m.

Overview of the Sleeping Beauty transposase system and how they are efficient tools for genome engineering.pl

June 30, 2015, 2 p.m.

Lambda Red mediated recombineering can be used to generate point mutations in plasmids and bacterial genomes. Learn how to use Lambda Red in this post.

Dec. 15, 2016, 3:57 p.m.

Epigenetic modifications are an additional layer of control over gene expression. Targeted epigenetic modification is possible using CRISPR tools from Addgene!

June 24, 2020, 5:45 p.m.

Learn how to incorporate phosphoserine into your protein of interest to study the effects of phosphorylation.

June 23, 2016, 2:30 p.m.

Acoustic reporter genes (ARGs) are activated by ultrasound instead of light. Updated bacterial and mammalians ARGs can provide contrast and improved utility.

Jan. 24, 2023, 2:15 p.m.

Learn about evaluating DNA quality with Spectophotometry using a Nanodrop

July 1, 2025, 1:15 p.m.