We narrowed to 356 results for: %s

1. R Bodies: Membrane-Rupturing Microscopic Tools

   Type

   Blog Post

   Published

   April 14, 2016, 2:30 p.m.

   ...This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive...

2. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...Xie K, Minkenberg B, Yang Y. Proc Natl Acad Sci U S A. 2015 Mar 17;112(11):3570-5. doi: 10.1073/pnas.1420294112...

3. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...input. Usually that function is in the shape of an S; there is a narrow threshold like a switch that turns...

4. Antibodies 101: Fab Fragments

   Type

   Blog Post

   Published

   Sept. 5, 2024, 1:15 p.m.

   ....1177/34.6.3084626  Zhang Q, Miyamoto A, Watanabe S, Arimori T, Sakai M, Tomisaki M, Kiuchi T, Takagi ...

5. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ... to do once you’ve chosen a gRNA and modification(s) Congratulations! You’ve picked your perfect guide...

6. Summer SciComm Series: Cognitive Load

   Type

   Blog Post

   Published

   June 16, 2022, 1:15 p.m.

   ...audience is expending to pay attention to the mode(s) of communication you choose.  Audience knowledge ...

7. What's New in CRISPR - November 2021

   Type

   Blog Post

   Published

   Nov. 18, 2021, 2:15 p.m.

   ...By Brook Pyhtila One drawback to the commonly used S. pyogenes CRISPR/Cas9 system is that it is too large...

8. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

   Type

   Blog Post

   Published

   Aug. 24, 2021, 1:15 p.m.

   ...References Boguszewska, K., Szewczuk, M., Urbaniak, S. et al. Review: immunoassays in DNA damage and instability...

9. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

   Type

   Blog Post

   Published

   Jan. 31, 2019, 1:32 p.m.

   ...microenvironments have started to gather attention. By the 1960’s, scientists have begun to understand two main areas...

10. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type

    Blog Post

    Published

    Nov. 3, 2020, 2:15 p.m.

    ...Together these two experiments determined that ChRger2’s improved light sensitivity and higher-photocurrent...

11. Viral Vectors 101: Inverted Terminal Repeats

    Type

    Blog Post

    Published

    Oct. 19, 2023, 1:15 p.m.

    ... guide AAV Viral Preps References  Chen, Y., Hu, S., Lee, W., Walsh, N., Iozza, K., Huang, N., Preston...

12. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...lentiviral backbone from the Zhang lab expressing S. pyogenes Cas9 and a gRNA cassette. It can be used...

13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...active or “on.” Dronpa’s color thus indicates MEK1’s current state (Green when off, dark when on). Figure...

14. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type

    Blog Post

    Published

    Jan. 11, 2017, 3:30 p.m.

    ...Many thanks to our guest blogger Gary McDowell! Gary S. McDowell is currently the Executive Director of Future...

15. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...Pubmed Central PMCID: PMC4000813. 2. McDowell, Gary S., et al. "Shaping the Future of Research: a perspective...

16. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ...that ultimately builds up to an overall conclusion(s). In some articles, the data are presented to build...

17. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ... the de novo NGS result to the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps ...

18. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type

    Blog Post

    Published

    Dec. 14, 2021, 2:15 p.m.

    ...https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi H, Horii T et al. (2016) Targeted DNA demethylation...

19. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...//doi.org/10.1089/hum.2019.277 Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD...

20. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type

    Blog Post

    Published

    Sept. 26, 2019, 12:50 p.m.

    ...tropism of Nanoblades, different viral envelope protein(s) are expressed to pseudotype Nanoblades. The team ...