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Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Addgene has a new brand identity! This post describes the design decisions that went into our new logo, style, introductory video, and our mascot, Blugene.

April 28, 2026, 1:15 p.m.

Curious about what it's like to be a summer intern at Addgenie? Addgenie Intern Toj explains it all!

Aug. 8, 2024, 7:22 p.m.

Transducions use viral vectors to insert nucleic acids into cells. Learn all about them in our Viral Vectors 101!

April 30, 2024, 1:15 p.m.

Find tips for hosting a great virtual lab meeting with ideas for online collaboration and space for brainstorming and discussion.

Feb. 9, 2021, 2:15 p.m.

The COVID-19 pandemic makes open science more important than ever. Read about how Addgene is weathering COVID-19 with support from the scientific community.

Sept. 17, 2020, 1:15 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Learn about new plasmids at Addgene including plasmids for CRISPR, sparse cell labeling, reporting calcineurin activity, and an alternative to DNA staining dyes.

June 2, 2020, 1:15 p.m.

Take behind-the-scenes look at the isolation of a thermostable Cas9, IgnaviCas9, from Yellowstone and learn about the advantages of using a thermostable Cas9 protein.

Nov. 12, 2019, 2 p.m.

The HA frankenbody combines optimized elements of an antibody probe with a fluorescent protein and can be used to easily visualize single molecules and newly formed proteins. 

Oct. 10, 2019, 12:41 p.m.

Resources to help improve precision genome editing through homology directed repair in experiments with CRISPR nucleases.

Feb. 14, 2018, 2:36 p.m.

TOPO cloning is a restriction enzyme and ligase free cloning method. Learn how TOPO works in this post.

Oct. 27, 2016, 2:30 p.m.

Advice on starting a lab from three PIs who have successfully built their own academic labs.

April 29, 2014, 1:43 p.m.

Thank you to all the researchers, labs, and scientists who have deposited with Addgene! Check out all the cool events we have planned to celebrate this week.

Dec. 12, 2022, 2:15 p.m.

This post, written by the creator of the CRISPRecise kit, explains how to use it to minimize off-target effects in CRISPR genome editing.

March 31, 2026, 1:15 p.m.

Learning to gate in a flow cytometry experiment can be difficult. Read our Intro to Gating post to help you get started!

April 4, 2024, 1:15 p.m.

Review of off-target effects in CRISPR experiments. Topics covered include: measuring, preventing, and controlling for off-target events.

Feb. 8, 2024, 2:15 p.m.

An introduction to conflict resolution, identifying types of conflict and strategies for solving them.

Jan. 4, 2022, 2:15 p.m.