We narrowed to 701 results for: plasmid

In the final post of our Quick Guide to Working with Drosophila, learn how to make Drosophila transgenic lines.

July 28, 2017, 1:30 p.m.

Addgene spoke to Kiran Musunuru about his new genome editing TALEN kit, the research the kit now makes possible, and how it compares to his CRISPR/Cas9 system.

Dec. 10, 2013, 3:13 p.m.

Learn how Addgene is supporting rare disease research through the Rare Genomics Institute and the BeHeard Award 2017.

July 20, 2017, 2:30 p.m.

Addgene recently attended the ASM Microbe 2016 conference in Boston. Check out what we learned!

July 7, 2016, 2:30 p.m.

Yeast genome modification plasmids have been developed for tagging yeast genes with fluorescent proteins, epitope tags, and more. Various systems are described to help you choose which to use for your research.

Nov. 19, 2013, 2:37 p.m.

Designing a CRISPR experiment in bacteira? Check out these tools for genome engineering, CRISPRa, CRISPRi, base editing, and microbiome engineering.

Sept. 28, 2020, noon

Dr. Le Cong, first author on a seminal CRISPR article from Dr. Feng Zhang's lab, answers FAQs about the genome editing technology.

March 13, 2014, 4:08 p.m.

Use these protocols and tips to make your own competent cells and transform your DNA. It's easy and affordable.

Jan. 28, 2014, 3:10 p.m.

Mobile-CRISPRi is a set of modular CRISPRi tools that can be used in diverse bacteria from various environments. It has the advantage of stable genomic integration and is easily transferred via conjugation.

April 4, 2019, 12:53 p.m.

Learn the basics of CRISPR ribonucleoprotein (RNP) preparation and delivery for your next gene editing experiment.

Sept. 6, 2018, 12:02 p.m.

Addgene scientists perform standard quality control for AAVs. But we are also going one step further with next-generation sequencing (NGS) of the viral genome.

Sept. 12, 2017, 1:44 p.m.

Science educator, rapper, and communicator Tom McFadden gives us SciComm tips and tell us all about science outreach company, Science with Tom.

Feb. 9, 2017, 3:30 p.m.

Curious about what an ITR is and why it's so important for an AAV? Learn all about inverted terminal repeats in this Viral Vectors 101 post.

Oct. 19, 2023, 1:15 p.m.

With it's small crRNAs and RNase activity, Cpf1 can easily be used for multiplex genome editing. Learn more about new multiplex Cpf1 tools here.

May 9, 2017, 2:12 p.m.

Model organisms have genetic and developmental characteristics that make them easy to study. Examples include E. coli, rat, frog, axolotl, and Arabidopsis.

Sept. 29, 2020, 1:15 p.m.

INTEGRATE is a bacterial genome engineering system that uses CRISPR to direct transposition to targeted locations in the genome.

Dec. 7, 2020, 2:53 p.m.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

July 31, 2020, 12:30 p.m.

Master the streak plate technique using a mixture of E. coli strains expressing a different chromoprotein.

March 2, 2021, 2:15 p.m.

Learn how to use the dTAG system to inducibly degrade your protein of interest.

June 21, 2018, 2:06 p.m.

Learn how you can use split-BioID to study protein-protein interactions and build a better understand of your protein's interactome.

May 1, 2018, 1:57 p.m.