We narrowed to 293 results for: promoter
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TypeBlog PostPublishedAug. 9, 2016, 2:30 p.m....regulatory regions in the genome, including enhancers, promoters, insulators, and more. I then identified valid...
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Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical abbreviations: Gr= Gracile tract, ... -
Plasmids 101: Control Plasmids
TypeBlog PostPublishedApril 9, 2015, 3:29 p.m....on the Addgene Blog Origin of Replication The Promoter Region – Let's Go! Browse All of Our Plasmids ... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....amino acid protein 12 Stability At N-terminus, promotes folding and structural integrity; cleavable. Not... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog PostPublishedAug. 5, 2014, 6:51 p.m....others understand ours. Improving access to data promotes dissemination, especially for young researchers... -
Using AAV for Neuronal Tracing
TypeBlog PostPublishedAug. 9, 2018, 1:04 p.m....toxicity. AAV vectors can be equipped with strong promoters for high and neuron-specific transgene expression...AAV2-retro vectors with a variety of transgene/promoter combinations. Check out our retrograde AAV blog... -
Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research
TypeBlog PostPublishedJuly 29, 2025, 1:15 p.m.... difference between them lies in the choice of promoter for ωRNA (reRNA) expression (see Figure 1). Both... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....binding and contributing to DNA unwinding. This promotes initiation at the plasmid ori, after which replication... -
An Introduction to Adenovirus
TypeBlog PostPublishedOct. 3, 2023, 1:15 p.m....from a replicating adenovirus using native viral promoters. Mol Ther, 7(4), 526–534. https:/doi.org/10.1016... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....beneficial for many systems. How the EDGE Program promotes scientific sharing The EDGE Program is designed... -
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View
TypeBlog PostPublishedOct. 19, 2017, 1:54 p.m....localization sequence) and expressing it from a CAGGs promoter. However FLPe is still less efficient than Cre... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....encoded on the envelope plasmid which contains a promoter, the envelope gene, and a polyA tail. ... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....iteron-containing plasmids, RepA can also bind to its own promoter and inhibit its own transcription. As copy number... -
Deep Mutational Scanning with One Pot Saturation Mutagenesis
TypeBlog PostPublishedFeb. 22, 2017, 3:30 p.m....interest. A low primer-to-template ratio is used to promote annealing of one primer to each template. The PCR... -
Changing Labor Laws Bring Increased Postdoc Wages
TypeBlog PostPublishedOct. 25, 2016, 2:30 p.m....help them navigate the scientific enterprise and promote their voices in driving changes in science. Follow... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....non-interview setting. There are many resources on self-promotion (how hard it is for some people, especially women... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m.... in mapping of regulatory elements (to mutate promoters/enhancers in reporter constructs), in functional... -
Tips for CRISPR Gene Editing in Mice
TypeBlog PostPublishedJune 28, 2016, 10:59 a.m....and 3′ EGFP fragments under the ubiquitous CAG promoter. The approximately 500 bp genomic fragment containing... -
Getting the Most from Your Lentiviral Transduction
TypeBlog PostPublishedApril 7, 2016, 2:30 p.m....enough to prevent confluency but high enough to promote cell growth. Overgrown cultures stimulate quiescence... -
Important Considerations in Optogenetics Behavioral Experiments
TypeBlog PostPublishedOct. 1, 2015, 2:30 p.m....reinforces behavior (activation of the circuit promotes subsequent, repeated activation), this is an approximation...